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La ricerca find articoli where soggetti phrase all words 'firefly luciferase' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 404 riferimenti
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    1. Hasan, MT; Schonig, K; Berger, S; Graewe, W; Bujard, H
      Long-term, noninvasive imaging of regulated gene expression in living mice

      GENESIS
    2. Seto, Y; Iba, T; Abe, K
      Development of ultra-high sensitivity bioluminescent enzyme immunoassay for prostate-specific antigen (PSA) using firefly luciferase

      LUMINESCENCE
    3. Balaguer, P; Boussioux, AM; Demirpence, E; Nicolas, JC
      Reporter cell lines are useful tools for monitoring biological activity ofnuclear receptor ligands

      LUMINESCENCE
    4. Eu, JY; Andrade, J
      Properties of firefly luciferase immobilized through a biotin carboxyl carrier protein domain

      LUMINESCENCE
    5. Elvang, AM; Westerberg, K; Jernberg, C; Jansson, JK
      Use of green fluorescent protein and luciferase biomarkers to monitor survival and activity of Arthrobacter chlorophenolicus A6 cells during degradation of 4-chlorophenol in soil

      ENVIRONMENTAL MICROBIOLOGY
    6. Patankar, S; Munasinghe, A; Shoaibi, A; Cummings, LM; Wirth, DF
      Serial analysis of gene expression in Plasmodium falciparum reveals the global expression profile of erythrocytic stages and the presence of anti-sense transcripts in the malarial parasite

      MOLECULAR BIOLOGY OF THE CELL
    7. Cherednikova, EY; Chikishev, AY; Dementieva, EI; Kossobokova, OV; Ugarova, NN
      Quenching of tryptophan fluorescence of firefly luciferase by substrates

      JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
    8. Weber, T; Marahiel, MA
      Exploring the domain structure of modular nonribosomal peptide synthetases

      STRUCTURE
    9. Barros, MP; Bechara, EJH
      Daily variations of antioxidant enzyme and luciferase activities in the luminescent click-beetle Pyrearinus termitilluminans: cooperation against oxygen toxicity

      INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
    10. Ehlting, J; Shin, JJK; Douglas, CJ
      Identification of 4-coumarate : coenzyme A ligase (4CL) substrate recognition domains

      PLANT JOURNAL
    11. Sankarasubramanian, S; Kaushik, BD
      Development of genetic markers in cyanobacteria and stability of genetically marked strains in soil

      WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
    12. Annicotte, JS; Schoonjans, K; Haby, C; Auwerx, J
      An E-box in pGL3 reporter vectors precludes their use for the study of sterol regulatory element-binding proteins

      BIOTECHNIQUES
    13. Zakhartchenko, VA; Anderson, WF; Rozenberg, Y
      Quantitation of retroviral-mediated transfer using luciferase in living and lysed cells

      BIOTECHNIQUES
    14. Karamohamed, S; Guidotti, G
      Bioluminometric method for real-time detection of ATPase activity

      BIOTECHNIQUES
    15. Henkel, R; Finkenzeller, C; Monsees, T; Franken, DR; Schill, WB; Miska, W
      Development of a new, highly sensitive zona pellucida binding assay using a bioluminescence-enhanced detection system

      ANDROLOGIA
    16. Gutendorf, B; Westendorf, J
      Comparison of an array of in vitro assays for the assessment of the estrogenic potential of natural and synthetic estrogens, phytoestrogens and xenoestrogens

      TOXICOLOGY
    17. Rastelli, L; Robinson, K; Xu, YB; Majumder, S
      Reconstitution of enhancer function in paternal pronuclei of one-cell mouse embryos

      MOLECULAR AND CELLULAR BIOLOGY
    18. van Roermund, CWT; Drissen, R; van den Berg, M; Ijlst, L; Hettema, EH; Tabak, HF; Waterham, HR; Wanders, RJA
      Identification of a peroxisomal ATP carrier required for medium-chain fatty acid beta-oxidation and normal peroxisome proliferation in Saccharomyces cerevisiae

      MOLECULAR AND CELLULAR BIOLOGY
    19. Waud, JP; Fajardo, AB; Sudhaharan, T; Trimby, AR; Jeffery, J; Jones, A; Campbell, AK
      Measurement of proteases using chemiluminescence-resonance-energy-transferchimaeras between green fluorescent protein and aequorin

      BIOCHEMICAL JOURNAL
    20. Sisido, M; Hohsaka, T
      Introduction of specialty functions by the position-specific incorporationof nonnatural amino acids into proteins through four-base codon/anticodon pairs

      APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
    21. Rasanen, LA; Elvang, AM; Jansson, J; Lindstrom, K
      Effect of heat stress on cell activity and cell morphology of the tropicalrhizobium, Sinorhizobium arboris

      FEMS MICROBIOLOGY ECOLOGY
    22. Cowan, HE; Falkinham, JO
      A luciferase-based method for assessing chlorine-susceptibility of Mycobacterium avium

      JOURNAL OF MICROBIOLOGICAL METHODS
    23. Dieckmann, R; Pavela-Vrancic, M; von Dohren, H
      Synthesis of (di)adenosine polyphosphates by non-ribosomal peptide synthetases (NRPS)

      BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
    24. Gupta, JC; Pandey, G; Mukherjee, KJ
      Two-stage cultivation of recombinant Saccharomyces cerevisiae to enhance plasmid stability under non-selective conditions: experimental study and modeling

      ENZYME AND MICROBIAL TECHNOLOGY
    25. Petanen, T; Virta, M; Karp, M; Romantschuk, M
      Construction and use of broad host range mercury and arsenite sensor plasmids in the soil bacterium Pseudomonas fluorescens OS8

      MICROBIAL ECOLOGY
    26. Manfredi, G; Spinazzola, A; Checcarelli, N; Naini, A
      Assay of mitochondrial ATP synthesis in animal cells

      METHODS IN CELL BIOLOGY, VOL 65
    27. Porcelli, AM; Pinton, P; Ainscow, EK; Chiesa, A; Rugolo, M; Rutter, GA; Rizzuto, R
      Targeting of reporter molecules to mitochondria to measure calcium, ATP, and pH

      METHODS IN CELL BIOLOGY, VOL 65
    28. Luque-Ortega, JR; Rivero-Lezcano, OM; Croft, SL; Rivas, L
      In vivo monitoring of intracellular ATP levels in Leishmania donovani promastigotes as a rapid method to screen drugs targeting bioenergetic metabolism

      ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
    29. Tenhami, M; Hakkila, K; Karp, M
      Measurement of effects of antibiotics in bioluminescent Staphylococcus aureus RN4220

      ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
    30. Carrick, K; Barney, M; Navarro, A; Ryder, D
      The comparison of four bioluminometers and their swab kits for instant hygiene monitoring and detection of microorganisms in the brewery

      JOURNAL OF THE INSTITUTE OF BREWING
    31. Nass, N; Scheel, D
      Enhanced luciferin entry causes rapid wound-induced light emission in plants expressing high levels of luciferase

      PLANTA
    32. Zhou, W; Edelman, GM; Mauro, VP
      Transcript leader regions of two Saccharomyces cerevisiae mRNAs contain internal ribosome entry sites that function in living cells

      PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    33. Kolb, VA
      Cotranslational protein folding

      MOLECULAR BIOLOGY
    34. Garlapati, S; Chou, J; Wang, CC
      Specific secondary structures in the capsid-coding region of giardiavirus transcript are required for its translation in Giardia lamblia

      JOURNAL OF MOLECULAR BIOLOGY
    35. van Leeuwen, W; Ruttink, T; Borst-Vrenssen, AWM; van der Plas, LHW; van der Krol, AR
      Characterization of position-induced spatial and temporal regulation of transgene promoter activity in plants

      JOURNAL OF EXPERIMENTAL BOTANY
    36. Sorensen, CE; Novak, I
      Visualization of ATP release in pancreatic acini in response to cholinergic stimulus - Use of fluorescent probes and confocal microscopy

      JOURNAL OF BIOLOGICAL CHEMISTRY
    37. Ramachandran, A; Jain, A; Arora, P; Bashyam, MD; Chatterjee, U; Ghosh, S; Parnaik, VK; Hasnain, SE
      Novel Sp family-like transcription factors are present in adult insect cells and are involved in transcription from the polyhedrin gene initiator promoter

      JOURNAL OF BIOLOGICAL CHEMISTRY
    38. Zako, T; Harada, K; Mannen, T; Yamaguchi, S; Kitayama, A; Ueda, H; Nagamune, T
      Monitoring of the refolding process for immobilized firefly luciferase with a biosensor based on surface plasmon resonance

      JOURNAL OF BIOCHEMISTRY
    39. Lagido, C; Pettitt, J; Porter, AJR; Paton, GI; Glover, LA
      Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors

      FEBS LETTERS
    40. Terlecky, SR; Legakis, JE; Hueni, SE; Subramani, S
      Quantitative analysis of peroxisomal protein import in vitro

      EXPERIMENTAL CELL RESEARCH
    41. Fahmi, NE; Golovine, S; Wang, BX; Hecht, SM
      Studies toward the site specific incorporation of sugars into proteins: Synthesis of glycosylated aminoacyl-tRNAs

      CARBOHYDRATE RESEARCH
    42. Eckenhoff, RG; Pidikiti, R; Reddy, KS
      Anesthetic stabilization of protein intermediates: Myoglobin and halothane

      BIOCHEMISTRY
    43. Wang, WQ; Xu, Q; Shan, YF; Xu, GJ
      Probing local conformational changes during equilibrium unfolding of firefly luciferase: Fluorescence and circular dichroism studies of single tryptophan mutants

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    44. Viviani, V; Uchida, A; Suenaga, N; Ryufuku, M; Ohmiya, Y
      Thr226 is a key residue for bioluminescence spectra determination in beetle luciferases

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    45. Zhang, Y; Stabernack, CR; Dutton, R; Sonner, J; Trudell, JR; Mihic, SJ; Yamakura, T; Harris, RA; Gong, D; Eger, EI
      Luciferase as a model for the site of inhaled anesthetic action

      ANESTHESIA AND ANALGESIA
    46. Ozawa, T; Kaihara, A; Sato, M; Tachihara, K; Umezawa, Y
      Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing

      ANALYTICAL CHEMISTRY
    47. Tanahashi, Y; Ohmiya, Y; Honma, S; Katsuno, Y; Ohta, H; Nakamura, H; Honma, K
      Continuous measurement of targeted promoter activity by a secreted bioluminescence reporter, Vargula hilgendorfii luciferase

      ANALYTICAL BIOCHEMISTRY
    48. Seto, Y; Ohkuma, H; Takayasu, S; Iba, T; Umeda, A; Abe, K
      Development of highly sensitive bioluminescent enzyme immunoassay with ultra-wide measurable range for thyroid-stimulating hormone using firefly luciferase

      ANALYTICA CHIMICA ACTA
    49. Contag, CH; Jenkins, D; Contag, FR; Negrin, RS
      Use of reporter genes for optical measurements of neoplastic disease in vivo

      NEOPLASIA
    50. Ohkuma, H; Abe, K; Kosaka, Y; Maeda, M
      Detection of luciferase having two kinds of luminescent colour based on optical filter procedure: application to an enzyme immunoassay

      LUMINESCENCE
    51. Charng, YC; Pfitzner, AJP; Pfitzner, UM; Charng-Chang, KF; Chen, CM; Tu, J; Kuo, TT
      Construction of an inducible transposon, INAc, to develop a gene tagging system in higher plants

      MOLECULAR BREEDING
    52. Lin, HS; van der Toorn, C; Raemakers, KJJM; Visser, RGF; De Jeu, MJ; Jacobsen, E
      Genetic transformation of Alstroemeria using particle bombardment

      MOLECULAR BREEDING
    53. Meredith, GD; Sims, CE; Soughayer, JS; Allbritton, NL
      Measurement of kinase activation in single mammalian cells

      NATURE BIOTECHNOLOGY
    54. Stack, JH; Whitney, M; Rodems, SM; Pollok, BA
      A ubiquitin-based tagging system for controlled modulation of protein stability

      NATURE BIOTECHNOLOGY
    55. Dugi, KA; Amar, MJA; Haudenschild, CC; Shamburek, RD; Bensadoun, A; Hoyt, RF; Fruchart-Najib, J; Madj, Z; Brewer, HB; Santamarina-Fojo, S
      In vivo evidence for both lipolytic and nonlipolytic function of hepatic lipase in the metabolism of HDL

      ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
    56. Hehl, AB; Marti, M; Kohler, P
      Stage-specific expression and targeting of cyst wall protein-green fluorescent protein chimeras in Giardia

      MOLECULAR BIOLOGY OF THE CELL
    57. Saito, H; Nakamura, H; Kato, S; Inoue, S; Inage, M; Ito, M; Tomoike, H
      Percutaneous in vivo gene transfer to the peripheral lungs using plasmid-liposome complexes

      AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
    58. Meissner, R; Chague, V; Zhu, QH; Emmanuel, E; Elkind, Y; Levy, AA
      A high throughput system for transposon tagging and promoter trapping in tomato

      PLANT JOURNAL
    59. Worley, CK; Zenser, N; Ramos, J; Rouse, D; Leyser, O; Theologis, A; Callis, J
      Degradation of Aux/IAA proteins is essential for normal auxin signalling

      PLANT JOURNAL
    60. Dan, M; Wang, AL; Wang, CC
      Inhibition of pyruvate-ferredoxin oxidoreductase gene expression in Giardia lamblia by a virus-mediated hammerhead ribozyme

      MOLECULAR MICROBIOLOGY
    61. Gurvitz, A; Langer, S; Piskacek, M; Hamilton, B; Ruis, H; Hartig, A
      Predicting the function and subcellular location of Caenorhabditis elegansproteins similar to Saccharomyces cerevisiae beta-oxidation enzymes

      YEAST
    62. Marron-Brignone, L; Morelis, RM; Chauvet, JP; Coulet, PR
      Insertion of luciferase in Langmuir-Blodgett films through enzyme-glycolipidic vesicles

      LANGMUIR
    63. Territo, PR; Mootha, VK; French, SA; Balaban, RS
      Ca2+ activation of heart mitochondrial oxidative phosphorylation: role of the F-0/F-1-ATPase

      AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
    64. Babincova, M; Sourivong, P; Babinec, P
      Gene transfer-mediated intracellular photodynamic therapy

      MEDICAL HYPOTHESES
    65. Lundovskikh, I; Dementieva, E; Ugarova, N
      Recombinant firefly luciferase in Escherichia coli - Properties and immobilization

      APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
    66. Valero, E; Varon, R; Garcia-Carmona, F
      Kinetics of a self-amplifying substrate cycle: ADP-ATP cycling assay

      BIOCHEMICAL JOURNAL
    67. Yu, DC; Wang, AL; Wang, CC
      The putative transcription initiation site in giardiavirus double-strandedRNA genome

      MOLECULAR AND BIOCHEMICAL PARASITOLOGY
    68. Sun, CH; Tai, JH
      Development of a tetracycline controlled gene expression system in the parasitic protozoan Giardia lamblia

      MOLECULAR AND BIOCHEMICAL PARASITOLOGY
    69. Dementieva, EI; Fedorchuk, EA; Brovko, LY; Savitskii, AP; Ugarova, NN
      Fluorescent properties of firefly luciferases and their complexes with luciferin

      BIOSCIENCE REPORTS
    70. Olesen, CEM; Martin, CS; Mosier, J; Liu, B; Voyta, JC; Bronstein, I
      Chemiluminescent reporter gene assays with 1,2-dioxetane enzyme substrates

      BIOLUMINESCENCE AND CHEMILUMINESCENCE, PT C
    71. Gelmini, S; Pinzani, P; Pazzagli, M
      Luciferase gene as reporter: Comparison with the CAT gene and use in transfection and microinjection of mammalian cells

      BIOLUMINESCENCE AND CHEMILUMINESCENCE, PT C
    72. Bessarab, IN; Liu, HW; Ip, CF; Tai, JH
      The complete cDNA sequence of a type II Trichomonas vaginalis virus

      VIROLOGY
    73. Viviani, VR; Ohmiya, Y
      Bioluminescence color determinants of Phrixothrix railroad-worm luciferases: Chimeric luciferases, site-directed mutagenesis of Arg 215 and guanidineeffect

      PHOTOCHEMISTRY AND PHOTOBIOLOGY
    74. Ulgiati, D; Subrata, LS; Abraham, LJ
      The role of Sp family members, basic Krupple-like factor, and E box factors in the basal and IFN-gamma regulated expression of the human complement C4 promoter

      JOURNAL OF IMMUNOLOGY
    75. Karpichev, IV; Small, GM
      Evidence for a novel pathway for the targeting of a Saccharomyces cerevisiae peroxisomal protein belonging to the isomerase/hydratase family

      JOURNAL OF CELL SCIENCE
    76. Tu, G; Kirchmaier, AL; Liggitt, D; Liu, Y; Liu, SQ; Yu, WH; Heath, TD; Thor, A; Debs, RJ
      Non-replicating Epstein-Barr virus-based plasmids extend gene expression and can improve gene therapy in vivo

      JOURNAL OF BIOLOGICAL CHEMISTRY
    77. Jardim, A; Liu, W; Zheleznova, E; Ullman, B
      Peroxisomal targeting signal-1 receptor protein PEX5 from Leishmania donovani - Molecular, biochemical, and immunocytochemical characterization

      JOURNAL OF BIOLOGICAL CHEMISTRY
    78. Zako, T; Deguchi, H; Kitayama, A; Ueda, H; Nagamune, T
      Refolding of firefly luciferase immobilized on agarose beads

      JOURNAL OF BIOCHEMISTRY
    79. Tyedmers, J; Kruse, M; Lerner, M; Demand, J; Hohfeld, J; Solsbacher, J; Volkmer, JR; Zimmermann, R
      Assembly of heterodimeric luciferase after de novo synthesis of subunits in rabbit reticulocyte lysate involves Hsc70 and Hsp40 at a post-translational stage

      EUROPEAN JOURNAL OF BIOCHEMISTRY
    80. Azoulay-Zohar, H; Aflalo, C
      Binding of rat brain hexokinase to recombinant yeast mitochondria - Identification of necessary physico-chemical determinants

      EUROPEAN JOURNAL OF BIOCHEMISTRY
    81. Lodder, M; Crasto, CF; Laikhter, AL; An, HY; Arslan, T; Karginov, VA; Short, GF; Hecht, SM
      Synthesis of aspartic acid derivatives useful for the preparation of misacylated transfer RNAs

      CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE
    82. Cigic, B; Dahl, SW; Pain, RH
      The residual pro-part of cathepsin C fulfills the criteria required for anintramolecular chaperone in folding and stabilizing the human proenzyme

      BIOCHEMISTRY
    83. Short, GF; Laikhter, AL; Lodder, M; Shayo, Y; Arslan, T; Hecht, SM
      Probing the S1/S1 ' substrate binding pocket geometry of HIV-1 protease with modified aspartic acid analogues

      BIOCHEMISTRY
    84. Koo, HM; Kim, YS
      Identification of active-site residues in Bradyrhizobium japonicum malonyl-coenzyme A synthetase

      ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
    85. Smith, AD; Trempe, JP
      Luminometric quantitation of Photinus pyralis firefly luciferase and Escherichia coli beta-galactosidase in blood-contaminated organ lysates

      ANALYTICAL BIOCHEMISTRY
    86. Zhang, XM; Kobatake, E; Kobayashi, K; Yanagida, Y; Aizawa, M
      Genetically fused protein A-luciferase for immunological blotting analyses

      ANALYTICAL BIOCHEMISTRY
    87. Dyer, BW; Ferrer, FA; Klinedinst, DK; Rodriguez, R
      A noncommercial dual luciferase enzyme assay system for reporter gene analysis

      ANALYTICAL BIOCHEMISTRY
    88. Ito, K; Nishimura, K; Murakami, S; Arakawa, H; Maeda, M
      Novel bioluminescent assay of pyruvate phosphate dikinase using firefly luciferase-luciferin reaction and its application to bioluminescent enzyme immunoassay

      ANALYTICA CHIMICA ACTA
    89. Funabashi, H; Imajo, T; Kojima, J; Kobatake, E; Aizawa, M
      Bioluminescent monitoring of intracellular ATP during fermentation

      LUMINESCENCE
    90. Hoffner, S; Jimenez-Misas, C; Lundin, A
      Improved extraction and assay of mycobacterial ATP for rapid drug susceptibility testing

      LUMINESCENCE
    91. Brovko, LY; Cherednikova, EY; Chikishev, AY; Dementieva, EI; Koroteev, NI; Ugarova, NN
      Transient increase of tryptophan fluorescence of enzyme caused by photoexcitation of ligand in luciferase-luciferin complex

      BIOSPECTROSCOPY
    92. Brovko, LY; Dement'eva, EI; Koroteev, NI; Ugarova, NN; Cherednikova, EY; Chikishev, AY
      Photoinduced enhancement of the tryptophan fluorescence of the enzyme in the luciferase-luciferin complex

      QUANTUM ELECTRONICS
    93. Knodler, LA; Svard, SG; Silberman, JD; Davids, BJ; Gillin, FD
      Developmental gene regulation in Giardia lamblia: first evidence for an encystation-specific promoter and differential 5 ' mRNA processing

      MOLECULAR MICROBIOLOGY
    94. Arakawa, H; Kokado, A; Yoshizawa, S; Maeda, M; Tokita, A; Yamashiro, Y
      Bioluminescent PCR-RFLP enzyme-linked immunosorbent assay for analysis of vitamin D receptor gene polymorphism

      ANALYTICAL SCIENCES
    95. Nabi, A; Yaqoob, M; Anwar, M
      Chemiluminescence and bioluminescence monitoring - A flow-injection approach

      LABORATORY ROBOTICS AND AUTOMATION
    96. Moller, G; Luders, J; Markus, M; Husen, B; Van Veldhoven, PP; Adamski, J
      Peroxisome targeting of porcine 17 beta-hydroxysteroid dehydrogenase type IV/D-specific multifunctional protein 2 is mediated by its C-terminal tripeptide AKI

      JOURNAL OF CELLULAR BIOCHEMISTRY
    97. Rudakoff, B; Undisz, K; Mayer, G; Sobek, L; Kaufmann, G; Thiericke, R; Grabley, S; Munder, T
      Dual reporter systems in yeast and mammalian cells for assessing progesterone receptor modulators

      JOURNAL OF CELLULAR BIOCHEMISTRY
    98. Elliott, AR; Campbell, JA; Dugdale, B; Brettell, RIS; Grof, CPL
      Green fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells

      PLANT CELL REPORTS
    99. Matsuki, H; Suzuki, A; Kamaya, H; Ueda, I
      Specific and non-specific binding of long-chain fatty acids to firefly luciferase: cutoff at octanoate

      BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
    100. Fontes, R; Sillero, MAG; Sillero, A
      Acyl-CoA synthetase catalyzes the synthesis of diadenosine hexaphosphate (Ap(6)A)

      BIOCHIMIE


ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/06/20 alle ore 05:13:42