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La ricerca find articoli where soggetti phrase all words 'enzymatic synthesis' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 961 riferimenti
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    1. Wegman, MA; Janssen, MHA; van Rantwijk, F; Sheldon, RA
      Towards biocatalytic synthesis of beta-lactam antibiotics

      ADVANCED SYNTHESIS & CATALYSIS
    2. Fernandez-Lafuente, R; Hernandez-Justiz, O; Mateo, C; Terreni, M; Fernandez-Lorente, G; Moreno, MA; Alonso, J; Garcia-Lopez, JL; Guisan, JM
      Biotransformations catalyzed by multimeric enzymes: Stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions

      BIOMACROMOLECULES
    3. Wen, S; Guo, Z
      Unprotected oligosaccharides as phase tags: Solution-phase synthesis of glycopeptides with solid-phase workups

      ORGANIC LETTERS
    4. Clapes, P; Espelt, L; Navarro, MA; Solans, C
      Highly concentrated water-in-oil emulsions as novel reaction media for protease-catalysed kinetically controlled peptide synthesis

      JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
    5. Roberts, SM
      Preparative biotransformations

      JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1
    6. Butler, T; Schumacher, T; Namdjou, DJ; Gallego, RG; Clausen, H; Elling, L
      Chemoenzymatic synthesis of biotinylated nucleotide sugars as substrates for glycosyltransferases

      CHEMBIOCHEM
    7. Kontogianni, A; Skouridou, V; Sereti, V; Stamatis, H; Kolisis, FN
      Regioselective acylation of flavonoids catalyzed by lipase in low toxicitymedia

      EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
    8. Nashiru, O; Zechel, DL; Stoll, D; Mohammadzadeh, T; Warren, RAJ; Withers, SG
      beta-Mannosynthase: Synthesis of beta-mannosides with a mutant beta-mannosidase

      ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
    9. Milgotina, EI; Shcheglov, AS; Lapa, GB; Chestukhina, GG; Voyushina, TL
      Enzymatic synthesis of chromogenic substrates for Glu,Asp-specific proteinases

      JOURNAL OF PEPTIDE RESEARCH
    10. Sakiyama, T; Yoshimi, T; Tanaka, A; Ozaki, S; Nakanishi, K
      Analysis of monoglyceride synthetic reaction in a solvent-free two-phase system catalyzed by a monoacylglycerol lipase from Pseudomonas sp LP7315

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    11. Patel, RN
      Enzymatic synthesis of chiral intermediates for Omapatrilat, an antihypertensive drug

      BIOMOLECULAR ENGINEERING
    12. Getun, IV; Filippova, IY; Lysogorskaya, EN; Oksenoit, ES
      SDS-subtilisin catalyzed synthesis of tetra-peptides containing multifunctional amino acid residues in ethanol

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    13. Yoon, JH; Ryu, GH; Finch, P; Rhee, JS
      Enzymatic synthesis of 3-O-methyl-4-O-beta-D-galactopyranosyl-D-glucose (3-O-methyl-lactose); a potential agent for the assessment of intestinal lactase activity

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    14. Illanes, A; Fajardo, A
      Kinetically controlled synthesis of ampicillin with immobilized penicillinacylase in the presence of organic cosolvents

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    15. Fernandez-Lafuente, R; Hernandez-Justiz, O; Mateo, C; Terreni, M; Alonso, J; Garcia-Lopez, JL; Moreno, MA; Guisan, JM
      Stabilization of a tetrameric enzyme (alpha-amino acid ester hydrolase from Acetobacter turbidans) enables a very improved performance of ampicillin synthesis

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    16. Cao, L; van Langen, LM; van Rantwijk, F; Sheldon, RA
      Cross-linked aggregates of penicillin acylase: robust catalysts for the synthesis of beta-lactam antibiotics

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    17. Chamouleau, F; Coulon, D; Girardin, M; Ghoul, M
      Influence of water activity and water content on sugar esters lipase-catalyzed synthesis in organic media

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    18. Nishimura, S
      Combinatorial syntheses of sugar derivatives

      CURRENT OPINION IN CHEMICAL BIOLOGY
    19. Riva, S
      Biocatalytic modification of natural products

      CURRENT OPINION IN CHEMICAL BIOLOGY
    20. Ahn, JE; Kim, C; Shin, CS
      Enzymic synthesis of aspartame precursors from eutectic substrate mixtures

      PROCESS BIOCHEMISTRY
    21. Krishna, SH; Sattur, AP; Karanth, NG
      Lipae-catalyzed synthesis of isoamyl isobutyrate - optimization using a central composite rotatable design

      PROCESS BIOCHEMISTRY
    22. Bellahouel, S; Rolland, V; Roumestant, ML; Viallefont, P; Martinez, J
      Chemoenzymatic synthesis of surfactants from carbohydrates, amino acids, and fatty acids

      PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
    23. Leclerc, GJ; Barredo, JC
      Folylpoly-gamma-glutamate synthetase gene mRNA splice variants and proteinexpression in primary human leukemia cells, cell lines, and normal human tissues

      CLINICAL CANCER RESEARCH
    24. Mayer, C; Jakeman, DL; Mah, M; Karjala, G; Gal, L; Warren, RAJ; Withers, SG
      Directed evolution of new glycosynthases from Agrobacterium beta-glucosidase: a general screen to detect enzymes for oligosaccharide synthesis

      CHEMISTRY & BIOLOGY
    25. Sanders, DAR; Staines, AG; McMahon, SA; McNeil, MR; Whitfield, C; Naismith, JH
      UDP-galactopyranose mutase has a novel structure and mechanism

      NATURE STRUCTURAL BIOLOGY
    26. Kolobanova, SV; Filippova, IY; Lysogorskaya, EN; Bacheva, AV; Oksenoit, ES; Stepanov, VM
      The enzymatic segment condensation of peptides on a solid phase in organicmedium

      RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
    27. Heinsman, NWJT; Teixeira, A; Van der Weide, PLJ; Franssen, MCR; Van der Padt, A; De Groot, A; Van 'T Riet, K
      Esterification of 4-methyloctanoic acid with polyethylene glycol at different a(w)

      BIOCATALYSIS AND BIOTRANSFORMATION
    28. Martin, MT; Alcalde, M; Plou, FJ; Dijkhuizen, L; Ballesteros, A
      Synthesis of malto-oligosaccharides via the acceptor reaction catalyzed bycyclodextrin glycosyltransferases

      BIOCATALYSIS AND BIOTRANSFORMATION
    29. Koller, G; Aris, MJ; Ujang, Z; Vaidya, AM
      Dependence of the synthetic activity of nine immobilized lipases on water activity

      BIOCATALYSIS AND BIOTRANSFORMATION
    30. Loos, K; Jonas, G; Stadler, R
      Carbohydrate modified polysiloxanes, 3 - Solution properties of carbohydrate-polysiloxane conjugates in toluene

      MACROMOLECULAR CHEMISTRY AND PHYSICS
    31. Kim, IH; Yoon, CS; Lee, KW
      Transesterification of conjugated linoleic acid and tricaprylin by lipasesin organic solvents

      FOOD RESEARCH INTERNATIONAL
    32. Albermann, C; Piepersberg, W
      Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine

      GLYCOBIOLOGY
    33. Jager, S; Brumbauer, A; Feher, E; Reczey, K; Kiss, L
      Production and characterization of beta-glucosidases from different Aspergillus strains

      WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
    34. Palcic, MM; Seto, NOL; Hindsgaul, O
      Natural and recombinant A and B gene encoded glycosyltransferases

      TRANSFUSION MEDICINE
    35. Fessner, WD; Helaine, V
      Biocatalytic synthesis of hydroxylated natural products using aldolases and related enzymes

      CURRENT OPINION IN BIOTECHNOLOGY
    36. Ulijn, RV; Janssen, AEM; Moore, BD; Halling, PJ
      Predicting when precipitation-driven synthesis is feasible: Application tobiocatalysis

      CHEMISTRY-A EUROPEAN JOURNAL
    37. Sekizaki, H; Itoh, K; Toyota, E; Tanizawa, K
      Chum salmon trypsin-catalyzed preferential formation of peptides containing D-amino acid

      AMINO ACIDS
    38. Leonida, MD
      Redox enzymes used in chiral syntheses coupled to coenzyme regeneration

      CURRENT MEDICINAL CHEMISTRY
    39. Nakagawa, T; Ito, M
      Distribution of neutral/alkaline ceramidase in marine invertebrates revealed by fluorescence-labeled ceramide

      FISHERIES SCIENCE
    40. Oikawa, T; Tsukagawa, Y; Chino, M; Soda, K
      Increased transglycosylation activity of Rhodotorula glutinis endo-beta-glucanase in media containing organic solvent

      BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
    41. Luic, M; Koellner, G; Shugar, D; Saenger, W; Bzowska, A
      Calf spleen purine nucleoside phosphorylase: structure of its ternary complex with an N(7)-acycloguanosine inhibitor and a phosphate anion

      ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
    42. Hansson, T; Adlercreutz, P
      Optimization of galactooligosaccharide production from lactose using beta-glycosidases from hyperthermophiles

      FOOD BIOTECHNOLOGY
    43. Zhou, CA; Tian, GL; Shen, HY; Ye, YH
      Effect of salt hydrates on enzymatic oligopeptide synthesis in organic solvents

      ACTA CHIMICA SINICA
    44. Kunos, G; Batkai, S
      Novel physiologic functions of endocannabinoids as revealed through the use of mutant mice

      NEUROCHEMICAL RESEARCH
    45. Augustyniak, W; Kanski, R; Kanska, M
      Synthesis of carbon-14 labeled [1-C-14]-, and [2-C-14]-L-tyrosine

      JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
    46. Milecki, J; Foldesi, A; Fischer, A; Adamiak, RW; Chattopadhyaya, J
      Synthesis of multiply labelled ribonucleosides for sequence-specific labelling of oligo-RNA

      JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
    47. Hausler, H; Stutz, AE
      D-xylose (D-glucose) isomerase and related enzymes in carbohydrate synthesis

      GLYOSCIENCE: EPIMERISATION, ISOMERISATION AND REARRANGEMENT REACTIONS OF CARBOHYDRATES
    48. Haneda, K; Inazu, T; Mizuno, M; Iguchi, R; Tanabe, H; Fujimori, K; Yamamoto, K; Kumagai, H; Tsumori, K; Munekata, E
      Chemo-enzymatic synthesis of a bioactive peptide containing a glutamine-linked oligosaccharide and its characterization

      BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
    49. Dion, M; Nisole, A; Spangenberg, P; Andre, C; Glottin-Fleury, A; Mattes, R; Tellier, C; Rabiller, C
      Modulation of the regioselectivity of a Bacillus alpha-galactosidase by directed evolution

      GLYCOCONJUGATE JOURNAL
    50. Ahmed, F; Stein, A; Lye, GJ
      In-situ product removal to enhance the yield of biocatalytic reactions with competing equilibria: alpha-glucosidase catalysed synthesis of disaccharides

      JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
    51. Fan, KK; Ouyang, PK; Wu, XJ; Lu, ZH
      Prediction of aqueous phase pH for enzymatic synthesis of peptides in aqueous-organic biphasic systems

      JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
    52. Fan, KK; Ouyang, P; Wu, XJ; Lu, ZH
      Enhancement of the activity of papain in mixed reverse micellar systems inthe presence of Tween80

      JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
    53. Kiran, KR; Divakar, S
      Lipase catalyzed synthesis of organic acid esters of lactic acid in non-aqueous media

      JOURNAL OF BIOTECHNOLOGY
    54. Porter, AC; Felder, CC
      The endocannabinoid nervous system: unique opportunities for therapeutic intervention

      PHARMACOLOGY & THERAPEUTICS
    55. Hansson, T; Andersson, M; Wehtje, E; Adlercreutz, P
      Influence of water activity on the competition between beta-glycosidase catalysed transglycosylation and hydrolysis in aqueous hexanol

      ENZYME AND MICROBIAL TECHNOLOGY
    56. Torres, C; Otero, C
      Part III. Direct enzymatic esterification of lactic acid with fatty acids

      ENZYME AND MICROBIAL TECHNOLOGY
    57. Lee, CK; Lee, ZS; Yang, PF
      Effect of cell membrane of Agrobacterium radiobacter on enhancing D-amino acids production from racemic hydantoins

      ENZYME AND MICROBIAL TECHNOLOGY
    58. Kim, C; Shin, CS
      Solvent-free enzymatic synthesis of alitame precursor using eutectic substrate mixtures

      ENZYME AND MICROBIAL TECHNOLOGY
    59. Fernandez-Perez, M; Otero, C
      Enzymatic synthesis of amide surfactants from ethanolamine

      ENZYME AND MICROBIAL TECHNOLOGY
    60. Pan, IH; Yao, HJ; Li, YK
      Effective extraction and purification of beta-xylosidase from Trichoderma koningii fermentation culture by aqueous two-phase partitioning

      ENZYME AND MICROBIAL TECHNOLOGY
    61. Patricelli, MP; Cravatt, BF
      Proteins regulating the biosynthesis and inactivation of neuromodulatory fatty acid amides

      VITAMINS AND HORMONES - ADVANCES IN RESEARCH AND APPLICATIONS, VOL 62
    62. Dotsch, V
      Protein-DNA interactions

      NUCLEAR MAGNETIC RESONANCE OF BIOLOGICAL MACROMOLECULES, PT B
    63. Kojima, C; Ono, AM; Ono, A; Kainosho, M
      Solid-phase synthesis of selectively labeled DNA: Applications for multidimensional nuclear magnetic resonance spectroscopy

      NUCLEAR MAGNETIC RESONANCE OF BIOLOGICA MACROMOLECULES, PT A
    64. Werner, MH; Gupta, V; Lambert, LJ; Nagata, T
      Uniform C-13/N-15-labeling of DNA by tandem repeat amplification

      NUCLEAR MAGNETIC RESONANCE OF BIOLOGICA MACROMOLECULES, PT A
    65. Live, B; Silks, LAP; Schmidt, J
      C-13 isotopic enrichment for nuclear magnetic resonance studies of carbohydrates and glycoconjugates

      NUCLEAR MAGNETIC RESONANCE OF BIOLOGICA MACROMOLECULES, PT A
    66. Steckhan, E; Arns, T; Heineman, WR; Hilt, G; Hoormann, D; Jorissen, J; Kroner, L; Lewall, B; Putter, H
      Environmental protection and economization of resources by electroorganic and electroenzymatic syntheses

      CHEMOSPHERE
    67. D'Andrea, F; Catelani, G; Mariani, M; Vecchi, B
      Concise synthesis of 1-deoxy-4-O-beta-D-galactopyranosyl-D-nojirimycin avoiding a glycosylation step

      TETRAHEDRON LETTERS
    68. Sarabia, F; Lopez-Herrera, FJ
      beta-Oxy-alpha-diazo-carbonyl compounds. Part 6: Synthesis and reactivity of 3-deoxy-2-diazo-esters derived from monosaccharides. Applications in thesynthesis of 2-C-alkyl-KDO analogues and related compounds

      TETRAHEDRON LETTERS
    69. Komba, S; Ito, Y
      beta-galactosidase-catalyzed intramolecular transglycosylation

      TETRAHEDRON LETTERS
    70. Xu, MH; Lin, ZM; Pu, L
      Construction of an ortho-phenol polymer

      TETRAHEDRON LETTERS
    71. Athawale, V; Manjrekar, N
      Mild chemo-enzymatic synthesis of polymer-supported cinchona alkaloids andtheir application in asymmetric Michael addition

      TETRAHEDRON LETTERS
    72. De Martin, L; Ebert, C; Gardossi, L; Linda, P
      High isolated yields in thermolysin-catalysed synthesis of Z-L-aspartyl-L-phenylalanine methyl ester in toluene at controlled water activity

      TETRAHEDRON LETTERS
    73. Hayes, MP; Hatala, PJ; Sherer, BA; Tong, XH; Zanatta, N; Borer, PN; Kallmerten, J
      Regioselective synthesis of C-13(1)-labeled 2-deoxyribonolactones

      TETRAHEDRON
    74. Potier, P; Bouchu, A; Descotes, G; Queneau, Y
      Lipase-catalysed selective synthesis of sucrose mixed diesters

      SYNTHESIS-STUTTGART
    75. Sears, P; Wong, CH
      Toward automated synthesis of oligosaccharides and glycoproteins

      SCIENCE
    76. Koeller, KM; Wong, CH
      Enzymes for chemical synthesis

      NATURE
    77. Day, TA; Rakhshan, F; Deutsch, DG; Barker, EL
      Role of fatty acid amide hydrolase in the transport of the endogenous cannabinoid anandamide

      MOLECULAR PHARMACOLOGY
    78. Liu, W; Cholli, AL; Kumar, J; Tripathy, S; Samuelson, L
      Mechanistic study of the peroxidase-catalyzed polymerization of sulfonatedphenol

      MACROMOLECULES
    79. Mitchell, SA; Pratt, MR; Hruby, VJ; Polt, R
      Solid-phase synthesis of O-linked glycopeptide analogues of enkephalin

      JOURNAL OF ORGANIC CHEMISTRY
    80. Blixt, O; Brown, J; Schur, MJ; Wakarchuk, W; Paulson, JC
      Efficient preparation of natural and synthetic galactosides with a recombinant beta-1,4-galactosyltransferase-/UDP-4 '-gal epimerase fusion protein

      JOURNAL OF ORGANIC CHEMISTRY
    81. Foldesi, A; Trifonova, A; Dinya, Z; Chattopadhyaya, J
      Total synthesis of 2 ',3 ',4 ',5 ',5 ''-H-2(5)-ribonucleosides: The key building blocks for NMR structure elucidation of large RNA

      JOURNAL OF ORGANIC CHEMISTRY
    82. Jackson, RG; Lim, EK; Li, Y; Kowalczyk, M; Sandberg, G; Hoggett, J; Ashford, DA; Bowles, DJ
      Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase

      JOURNAL OF BIOLOGICAL CHEMISTRY
    83. Ueda, N; Yamanaka, K; Yamamoto, S
      Purification and characterization of an acid amidase selective for N-palmitoylethanolamine, a putative endogenous anti-inflammatory substance

      JOURNAL OF BIOLOGICAL CHEMISTRY
    84. Wakarchuk, WW; Watson, D; St Michael, F; Li, JJ; Wu, YY; Brisson, JR; Young, NM; Gilbert, M
      Dependence of the bi-functional nature of a sialyltransferase from Neisseria meningitidis on a single amino acid substitution

      JOURNAL OF BIOLOGICAL CHEMISTRY
    85. Graeff, R; Munshi, C; Aarhus, R; Johns, M; Lee, HC
      A single residue at the active site of CD38 determines its NAD cyclizing and hydrolyzing activities

      JOURNAL OF BIOLOGICAL CHEMISTRY
    86. Zhang, YM; Rao, MS; Heath, RJ; Price, AC; Olson, AJ; Rock, CO; White, SW
      Identification and analysis of the acyl carrier protein (ACP) docking siteon beta-ketoacyl-ACP synthase III

      JOURNAL OF BIOLOGICAL CHEMISTRY
    87. Sugihara, A; Shimada, Y; Sugihara, S; Nagao, T; Watanabe, Y; Tominaga, Y
      A novel alpha-amino-acid esterase from Bacillus mycoides capable of forming peptides of DD- and DL-configurations

      JOURNAL OF BIOCHEMISTRY
    88. Chang, RC; Chou, SJ; Shaw, JF
      Synthesis of fatty acid esters by recombinant Staphylococcus epidermidis lipases in aqueous environment

      JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    89. Espin, JC; Soler-Rivas, C; Cantos, E; Tomas-Barberan, FA; Wichers, HJ
      Synthesis of the antioxidant hydroxytyrosol using tyrosinase as biocatalyst

      JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    90. Steinke, G; Weitkamp, P; Klein, E; Mukherjee, KD
      High-yield preparation of wax esters via lipase-catalyzed esterification using fatty acids and alcohols from crambe and camelina oils

      JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
    91. Ernst, B; Dragic, Z; Marti, S; Muller, C; Wagner, B; Jahnke, W; Magnani, JL; Norman, KE; Oehrlein, R; Peters, T; Kolb, HC
      Design and synthesis of E-selectin antagonists

      CHIMIA
    92. Fukase, K; Yasukochi, T; Kusumoto, S
      Chemoenzymatic synthesis of a trisaccharide-serine conjugate, Gal(beta 1-3)Gal(beta 1-4)Xyl(beta 1-O)-L-Ser, use of galactosyl fluoride as a donor for transglycosylation

      BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
    93. Amann, S; Drager, G; Rupprath, C; Kirschning, A; Elling, L
      (Chemo)enzymatic synthesis of dTDP-activated 2,6-dideoxysugars as buildingblocks of polyketide antibiotics

      CARBOHYDRATE RESEARCH
    94. Scherrmann, MC; Boutboul, A; Estramareix, B; Hoffmann, AS; Lubineau, A
      Binding properties and esterase activity of monoclonal antibodies elicitedagainst sucrose 6-heptylphosphonate

      CARBOHYDRATE RESEARCH
    95. Albermann, C; Piepersberg, W; Wehmeier, UF
      Synthesis of the milk oligosaccharide 2 '-fucosyllactose using recombinantbacterial enzymes

      CARBOHYDRATE RESEARCH
    96. Ma, XY; Stockigt, J
      High yielding one-pot enzyme-catalyzed synthesis of UDP-glucose in gram scales

      CARBOHYDRATE RESEARCH
    97. Endo, T; Koizumi, S; Tabata, K; Kakita, S; Ozaki, A
      Large-scale production of the carbohydrate portion of the sialyl-Tn epitope, alpha-Neup5Ac-(2 -> 6)-D-GalpNAc, through bacterial coupling

      CARBOHYDRATE RESEARCH
    98. Hara, Y; Suyama, K
      Biosynthesis of lactosamine in bovine mammary gland

      CARBOHYDRATE RESEARCH
    99. Degn, P; Zimmermann, W
      Optimization of carbohydrate fatty acid ester synthesis in organic media by a lipase from Candida antarctica

      BIOTECHNOLOGY AND BIOENGINEERING
    100. Youshko, MI; van Langen, LM; de Vroom, E; van Rantwijk, F; Sheldon, RA; Svedas, VK
      Highly efficient synthesis of ampicillin in an "aqueous solution-precipitate" system: Repetitive addition of substrates in a semicontinuous process

      BIOTECHNOLOGY AND BIOENGINEERING


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Documento generato il 23/10/20 alle ore 05:09:46