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La ricerca find articoli where soggetti phrase all words 'apoptosis' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 35013 riferimenti
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    1. Barash, I; Reichenstein, M
      Real-time imaging of beta-lactoglobulin-targeted luciferase activity in the mammary glands of transgenic mice

      MOLECULAR REPRODUCTION AND DEVELOPMENT
    2. Kamjoo, M; Brison, DR; Kimber, SJ
      Apoptosis in the preimplantation mouse embryo: Effect of strain differenceand in vitro culture

      MOLECULAR REPRODUCTION AND DEVELOPMENT
    3. Steinbach, JP; Supra, P; Huang, HJS; Cavenee, WK; Weller, M
      CD95-mediated apoptosis of human glioma cells: Modulation by epidermal growth factor receptor activity

      BRAIN PATHOLOGY
    4. Telbisz, A; Kovacs, AL; Somosy, Z
      Influence of X-ray on the autophagic-lysosomal system in rat pancreatic acini

      MICRON
    5. Miller, ML; Andringa, A; Dixon, K; Carty, MP
      Insights into UV-induced apoptosis: ultrastructure, trichrome stain and spectral imaging

      MICRON
    6. Meyer-Rochow, VB; Kashiwagi, T; Eguchi, E
      Selective photoreceptor damage in four species of insects induced by experimental exposures to UV-irradiation

      MICRON
    7. Bortul, R; Aluigi, M; Tazzari, PL; Tabellini, G; Baldini, G; Bareggi, R; Narducci, P; Martelli, AM
      Phosphoinositide-specific phospholipase C beta 1 expression is not linked to nerve growth factor-induced differentiation, cell survival or cell cyclecontrol in PC12 rat pheocromocytoma cells

      JOURNAL OF CELLULAR BIOCHEMISTRY
    8. Liu, Y; Wang, XH; Lo, AKF; Wong, YC; Cheung, ALM; Tsao, SW
      Latent membrane protein-1 of Epstein-Barr virus inhibits cell growth and induces sensitivity to cisplatin in nasopharyngeal carcinoma cells

      JOURNAL OF MEDICAL VIROLOGY
    9. Yamato, M; Konno, C; Utsumi, M; Kikuchi, A; Okano, T
      Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture

      BIOMATERIALS
    10. Ciapetti, G; Granchi, D; Savarino, L; Cenni, E; Magrini, E; Baldini, N; Giunti, A
      In vitro testing of the potential for orthopedic bone cements to cause apoptosis of osteoblast-like cells

      BIOMATERIALS
    11. Koob, TJ; Hernandez, DJ
      Material properties of polymerized NDGA-collagen composite fibers: development of biologically based tendon constructs

      BIOMATERIALS
    12. Signorelli, P; Hannun, YA
      Analysis and quantitation of ceramide

      G PROTEIN PATHWAYS: PT C, EFFECTOR MECHANISMS
    13. Habtemariam, S
      Hamamelitannin from Hamamelis virginiana inhibits the tumour necrosis factor-alpha (TNF)-induced endothelial cell death in vitro

      TOXICON
    14. Arrode, G; Boccaccio, C; Abastado, JP; Davrinche, C
      Cross-presentation of human cytomegalovirus pp65 (UL83) to CD8(+) T cells is regulated by virus-induced, soluble-mediator-dependent maturation of dendritic cells

      JOURNAL OF VIROLOGY
    15. Zhang, BY; Spandau, DF; Roman, A
      E5 protein of human papillomavirus type 16 protects human foreskin keratinocytes from UVB-irradiation-induced apoptosis

      JOURNAL OF VIROLOGY
    16. Higuchi, M; Kieff, E; Izumi, KM
      The Epstein-Barr virus latent membrane protein 1 putative Janus kinase 3 (JAK3) binding domain does not mediate JAK3 association or activation in B-lymphoma or lymphoblastoid cell lines

      JOURNAL OF VIROLOGY
    17. Kim, JY; Choi, JA; Kim, TH; Yoo, YD; Kim, JI; Lee, YJ; Yoo, SY; Cho, CK; Lee, YS; Lee, SJ
      Involvement of p38 mitogen-activated protein kinase in the cell growth inhibition by sodium arsenite

      JOURNAL OF CELLULAR PHYSIOLOGY
    18. Wentz-Hunter, K; Ueda, J; Shimizu, N; Yue, BYJT
      Myocilin is associated with mitochondria in human trabecular meshwork cells

      JOURNAL OF CELLULAR PHYSIOLOGY
    19. Lin, H; Liu, XY; Subramanian, B; Nakeff, A; Valeriote, F; Chen, BD
      Mitotic arrest induced by XK469, a novel antitumor agent, is correlated with the inhibition of cyclin B1 ubiquitination

      INTERNATIONAL JOURNAL OF CANCER
    20. Suri, S; Monkkonen, J; Taskinen, M; Pesonen, J; Blank, MA; Phipps, RJ; Rogers, MJ
      Nitrogen-containing bisphosphonates induce apoptosis of Caco-2 cells in vitro by inhibiting the mevalonate pathway: A model of bisphosphonate-inducedgastrointestinal toxicity

      BONE
    21. Ito, M; Amizuka, N; Nakajima, T; Ozawa, H
      Bisphosphonate acts on osteoclasts independent of ruffled borders in osteosclerotic (oc/oc) mice

      BONE
    22. Benford, HL; McGowan, NWA; Helfrich, MH; Nuttall, ME; Rogers, MJ
      Visualization of bisphosphonate-induced caspase-3 activity in apoptotic osteoclasts in vitro

      BONE
    23. Pereira, RMR; Delany, AM; Canalis, E
      Cortisol inhibits the differentiation and apoptosis of osteoblasts in culture

      BONE
    24. Mansfield, K; Teixeira, CC; Adams, CS; Shapiro, IM
      Phosphate ions mediate chondrocyte apoptosis through a plasma membrane transporter mechanism

      BONE
    25. Kok, LP
      Boon's disease: Hemorrhagic cystitis in conjunction with massive exfoliation of degenerated urothelial cells (apoptosis?) during intercontinental flights in an otherwise healthy person

      DIAGNOSTIC CYTOPATHOLOGY
    26. Hayashi, T; Faustman, DL
      Genome and hormones: Gender differences in physiology selected contribution: Association of gender-related LMP2 inactivation with autoimmune pathogenesis

      JOURNAL OF APPLIED PHYSIOLOGY
    27. Tesfaigzi, Y; Rudolph, K; Fischer, MJ; Conn, CA
      Bcl-2 mediates sex-specific differences in recovery of mice from LPS-induced signs of sickness independent of IL-6

      JOURNAL OF APPLIED PHYSIOLOGY
    28. Levin, ER
      Genome and hormones: Gender differences in physiology - Invited review: Cell localization, physiology, and nongenomic actions of estrogen receptors

      JOURNAL OF APPLIED PHYSIOLOGY
    29. Beauchamp, MH; Martinez-Bermudez, AK; Gobeil, F; Marrache, AM; Hou, X; Speranza, G; Abran, D; Quiniou, C; Lachapelle, P; Roberts, J; Almazan, G; Varma, DR; Chemtob, S
      Role of thromboxane in retinal microvascular degeneration in oxygen-induced retinopathy

      JOURNAL OF APPLIED PHYSIOLOGY
    30. Bredesen, DE
      Neurodegenerative disease and cancer: Two sides of a coin?

      HOSPITAL PRACTICE
    31. Doucet-Chabeaud, G; Godon, C; Brutesco, C; de Murcia, G; Kazmaier, M
      Ionising radiation induces the expression of PARP-1 and PARP-2 genes in Arabidopsis

      MOLECULAR GENETICS AND GENOMICS
    32. Gail, M; Gross, U; Bohne, W
      Transcriptional profile of Toxoplasma gondii-infected human fibroblasts asrevealed by gene-array hybridization

      MOLECULAR GENETICS AND GENOMICS
    33. Rudack, C
      The influence of cytokines and chemokines on inflammations of the upper airways

      LARYNGO-RHINO-OTOLOGIE
    34. Floreani, A; Guido, M; Bortolami, M; Della Zentil, G; Venturi, C; Pennelli, N; Naccarato, R
      Relationship between apoptosis, tumour necrosis factor, and cell proliferation in chronic cholestasis

      DIGESTIVE AND LIVER DISEASE
    35. Marucci, L; Ugili, L; Macarri, G; Feliciangeli, G; Bendia, E; Jezequel, AM; Orlandi, F; Benedetti, A
      Primary biliary cirrhosis: modalities of injury and death in biliary epithelium

      DIGESTIVE AND LIVER DISEASE
    36. Ballardini, G; Guidi, M; Susca, M; Ghetti, S; Grassi, A; Lari, F; Fusconi, M; Zauli, D; Bianchi, FB
      Bile duct cell apoptosis is a rare event in primary biliary cirrhosis

      DIGESTIVE AND LIVER DISEASE
    37. Trobonjaca, Z; Radosevic-Stasic, B; Crncevic, Z; Rukavina, D
      Modulatory effects of octreotide on anti-CD3 and dexamethasone-induced apoptosis of murine thymocytes

      INTERNATIONAL IMMUNOPHARMACOLOGY
    38. D'Agostino, P; Ferlazzo, V; Milano, S; La Rosa, M; Di Bella, G; Caruso, R; Barbera, C; Grimaudo, S; Tolomeo, M; Feo, S; Cillari, E
      Anti-inflammatory effects of chemically modified tetracyclines by the inhibition of nitric oxide and interleukin-12 synthesis in J774 cell line

      INTERNATIONAL IMMUNOPHARMACOLOGY
    39. Athanassakis, I; Mouratidou, M; Sakka, P; Evangeliou, A; Spilioti, M; Vassiliadis, S
      L-carnitine modifies the humoral immune response in mice after in vitro orin vivo treatment

      INTERNATIONAL IMMUNOPHARMACOLOGY
    40. Granville, DJ; Jiang, HJ; McManus, BM; Hunt, DWC
      Fas ligand and TRAIL augment the effect of photodynamic therapy on the induction of apoptosis in JURKAT cells

      INTERNATIONAL IMMUNOPHARMACOLOGY
    41. Kroncke, KD; Fehsel, K; Suschek, C; Kolb-Bachofen, V
      Inducible nitric oxide synthase-derived nitric oxide in gene regulation, cell death and cell survival

      INTERNATIONAL IMMUNOPHARMACOLOGY
    42. Kim, PKM; Zamora, R; Petrosko, P; Billiar, TR
      The regulatory role of nitric oxide in apoptosis

      INTERNATIONAL IMMUNOPHARMACOLOGY
    43. Blanco, L; Puente, J; Carrasco, C; Miranda, D; Wolf, ME; Mosnaim, AD
      Effect of salmonella-infected human monocytes on natural killer cell cytotoxicity. In vitro studies

      INTERNATIONAL IMMUNOPHARMACOLOGY
    44. Watson, CS; Gametchu, B
      Membrane estrogen and glucocorticoid receptors - implications for hormonalcontrol of immune function and autoimmunity

      INTERNATIONAL IMMUNOPHARMACOLOGY
    45. Harrison, MT; McCoy, KL
      Immunosuppression by arsenic: a comparison of cathepsin L inhibition and apoptosis

      INTERNATIONAL IMMUNOPHARMACOLOGY
    46. Samardzic, T; Stosic-Grujicic, S; Raicevic, N; Trajkovic, V
      Tumor cell-specific inhibition of inducible nitric oxide synthase activation by tiazofurin

      INTERNATIONAL IMMUNOPHARMACOLOGY
    47. Dulos, GJ; Bagchus, WM
      Androgens indirectly accelerate thymocyte apoptosis

      INTERNATIONAL IMMUNOPHARMACOLOGY
    48. Oyaizu, H; Adachi, Y; Yasumizu, R; Ono, M; Ikebukuro, K; Fukuhara, S; Ikehara, S
      Protection of T cells from radiation-induced apoptosis by Cepharanthin (R)

      INTERNATIONAL IMMUNOPHARMACOLOGY
    49. Hirano, T; Horigome, A; Takatani, M; Oka, K
      Cortisone counteracts apoptosis-inducing effect of cortisol in human peripheral-blood mononuclear cells

      INTERNATIONAL IMMUNOPHARMACOLOGY
    50. Zvetkova, E; Wirleitner, B; Tram, NT; Schennach, H; Fuchs, D
      Aqueous extracts of Crinum latifolium (L.) and Camellia sinensis show immunomodulatory properties in human peripheral blood mononuclear cells

      INTERNATIONAL IMMUNOPHARMACOLOGY
    51. Pompeia, C; Boaventura, MFC; Curi, R
      Antiapoptotic effect of dipyrone on HL-60, Jurkat and Raji cell lines submitted to UV irradiation, arachidonic acid and cycloheximide treatments

      INTERNATIONAL IMMUNOPHARMACOLOGY
    52. Jenkins, JK; Suwannaroj, S; Elbourne, KB; Ndebele, K; McMurray, RW
      17-beta-estradiol alters Jurkat lymphocyte cell cycling and induces apoptosis through suppression of Bcl-2 and cyclin A

      INTERNATIONAL IMMUNOPHARMACOLOGY
    53. Yui, S; Ubukata, K; Hodono, K; Kitahara, M; Mimaki, Y; Kuroda, M; Sashida, Y; Yamazaki, M
      Macrophage-oriented cytotoxic activity of novel triterpene saponins extracted from roots of Securidaca inappendiculata

      INTERNATIONAL IMMUNOPHARMACOLOGY
    54. Fujino, M; Li, XK; Guo, L; Amano, T; Suzuki, S
      Activation of caspases and mitochondria in FTY720-mediated apoptosis in human T cell line Jurkat

      INTERNATIONAL IMMUNOPHARMACOLOGY
    55. Johnson, VJ; Sharma, RP
      Gender-dependent immunosuppression following subacute exposure to fumonisin B-1

      INTERNATIONAL IMMUNOPHARMACOLOGY
    56. Korver, GE; Kam, CM; Powers, JC; Hudig, D
      Dipeptide vinyl sulfones suitable for intracellular inhibition of dipeptidyl peptidase I

      INTERNATIONAL IMMUNOPHARMACOLOGY
    57. Liu, J; Yoshikawa, H; Nakajima, Y; Tasaka, K
      Involvement of mitochondrial permeability transition and caspase-9 activation in dimethyl sulfoxide-induced apoptosis of EL-4 lymphoma cells

      INTERNATIONAL IMMUNOPHARMACOLOGY
    58. Verhasselt, V; Goldman, M
      New concepts on the pathogenesis of autoimmune disease

      ISRAEL MEDICAL ASSOCIATION JOURNAL
    59. Khalili, K; Croul, S; Del Valle, L; Krynska, B; Gordon, J
      Oncogenic potential of human neurotropic virus: Laboratory and clinical observations

      ISRAEL MEDICAL ASSOCIATION JOURNAL
    60. Lu, L; Reinach, PS; Kao, WWY
      Corneal epithelial wound healing

      EXPERIMENTAL BIOLOGY AND MEDICINE
    61. Gomez, G; Lee, HM; He, Q; Englander, EW; Uchida, T; Greeley, GH
      Acute pancreatitis signals activation of apoptosis-associated and survivalgenes in mice

      EXPERIMENTAL BIOLOGY AND MEDICINE
    62. Anderson, KM; Harris, JE
      Selected features of nonendocrine pancreatic cancer

      EXPERIMENTAL BIOLOGY AND MEDICINE
    63. Li, MY; Absher, PM; Liang, P; Russell, JC; Sobel, BE; Fukagawa, NK
      High glucose concentrations induce oxidative damage to mitochondrial DNA in explanted vascular smooth muscle cells

      EXPERIMENTAL BIOLOGY AND MEDICINE
    64. Gursoy, G; Cardounel, A; Hu, Y; Kalimi, M
      Biological effects of long-term caloric restriction: Adaptation with simultaneous administration of caloric stress plus repeated immobilization stress in rats

      EXPERIMENTAL BIOLOGY AND MEDICINE
    65. Xu, DZ; Wang, L; Olson, JE; Lu, L
      Asymmetrical response of p38 kinase activation to volume changes in primary rat astrocytes

      EXPERIMENTAL BIOLOGY AND MEDICINE
    66. Hakki, A; Pennypacker, K; Eidizadeh, S; Friedman, H; Pross, S
      Nicotine inhibition of apoptosis in murine immune cells

      EXPERIMENTAL BIOLOGY AND MEDICINE
    67. Wang, JF; Ren, XL; DeAngelis, J; Min, JY; Zhang, YF; Hampton, TG; Amende, I; Morgan, JP
      Differential patterns of cocaine-induced organ toxicity in murine heart versus liver

      EXPERIMENTAL BIOLOGY AND MEDICINE
    68. Vesely, MD; Gower, WR; Perez-Lamboy, G; Overton, RM; Graddy, L; Vesely, DL
      Evidence for an atrial natriuretic peptide-like gene in plants

      EXPERIMENTAL BIOLOGY AND MEDICINE
    69. Wang, JF; Olson, ME; Reno, CR; Wright, JB; Hart, DA
      The pig as a model for excisional skin wound healing: Characterization of the molecular and cellular biology, and bacteriology of the healing process

      COMPARATIVE MEDICINE
    70. Mahapatra, PK; Mohanty-Hejmadi, P; Chainy, GBN
      Changes in oxidative stress parameters and acid phosphatase activity in the pre-regressing and regressing tail of Indian jumping frog Polypedates maculatus Anura, Rhacophoridae)

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
    71. Venturino, A; Anguiano, OL; Gauna, L; Cocca, C; Bergoc, RM; de D'Angelo, AMP
      Thiols and polyamines in the potentiation of malathion toxicity in larval stages of the toad Bufo arenarum

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
    72. Anwar, MM; Moustafa, MA
      The effect of melatonin on eye lens of rats exposed to ultraviolet radiation

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
    73. Maugras, M; Infante, MR; Gerardin, C; Selve, C; Vinardell, MP
      Possible effects of counterions on biological activities of anionic surfactants

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
    74. Berntssen, MHG; Aspholm, OO; Hylland, K; Bonga, SEW; Lundebye, AK
      Tissue metallothionein, apoptosis and cell proliferation responses in Atlantic salmon (Salmo salar L.) parr fed elevated dietary cadmium

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
    75. da Silva, NJ; Aird, SD
      Prey specificity, comparative lethality and compositional differences of coral snake venoms

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
    76. Ermann, J; Fathman, CG
      Autoimmune diseases: genes, bugs and failed regulation

      NATURE IMMUNOLOGY
    77. Wu, L; Strasser, A
      "Decisions, decisions ...": beta-catenin-mediated activation of TCF-I and Lef-I influences the fate of developing T cells

      NATURE IMMUNOLOGY
    78. Yang, JH; Lin, Y; Guo, ZJ; Cheng, JK; Huang, JY; Deng, L; Liao, W; Chen, ZJ; Liu, ZG; Su, B
      The essential role of MEKK3 in TNF-induced NF-kappa B activation

      NATURE IMMUNOLOGY
    79. Boothby, M
      Specificity of SN50 for NF-kappa B?

      NATURE IMMUNOLOGY
    80. Ray, A
      Specificity of SN50 for NF-kappa B? Response

      NATURE IMMUNOLOGY
    81. Miyazaki, T; Reed, JC
      A GTP-binding adaptor protein couples TRAIL receptors to apoptosis-inducing proteins

      NATURE IMMUNOLOGY
    82. Xu, XN; Screaton, G
      HIV-1 Nef: negative effector of Fas?

      NATURE IMMUNOLOGY
    83. Mitchell, TC; Hildeman, D; Kedl, RM; Teague, TK; Schaefer, BC; White, J; Zhu, YN; Kappler, J; Marrack, P
      Immunological adjuvants promote activated T cell survival via induction ofBcl-3

      NATURE IMMUNOLOGY
    84. Aifantis, I; Gounari, F; Scorrano, L; Borowski, C; von Boehmer, H
      Constitutive pre-TCR signaling promotes differentiation through Ca2+ mobilization and activation of NF-kappa B and NFAT

      NATURE IMMUNOLOGY
    85. Aoki, K; Kurooka, M; Chen, JJ; Petryniak, J; Nabel, EG; Nabel, GJ
      Extracellular matrix interacts with soluble CD95L: Retention and enhancement of cytotoxicity

      NATURE IMMUNOLOGY
    86. Stassi, G; De Maria, R
      Thyrocytes - not innocent bystanders in autoimmune disease - Response

      NATURE IMMUNOLOGY
    87. Kee, BL; Rivera, RR; Murre, C
      Id3 inhibits B lymphocyte progenitor growth and survival in response to TGF-beta

      NATURE IMMUNOLOGY
    88. Makrigiannakis, A; Zoumakis, E; Kalantaridou, S; Coutifaris, C; Margioris, AN; Coukos, G; Rice, KC; Gravanis, A; Chrousos, GP
      Corticotropin-releasing hormone promotes blastocyst implantation and earlymaternal tolerance

      NATURE IMMUNOLOGY
    89. Kishimoto, H; Sprent, J
      A defect in central tolerance in NOD mice

      NATURE IMMUNOLOGY
    90. Rosen, LS; Gordon, D; Kaminski, M; Howell, A; Belch, A; Mackey, J; Apffelstaedt, J; Hussein, M; Coleman, RE; Reitsma, DJ; Seaman, JJ; Chen, BL; Ambros, Y
      Zoledronic acid versus pamidronate in the treatment of skeletal metastasesin patients with breast cancer or osteolytic lesions of multiple myeloma: A phase III, double-blind, comparative trial

      CANCER JOURNAL
    91. Watanabe, G; Kaganoi, J; Imamura, M; Shimada, Y; Itami, A; Uchida, S; Sato, F
      Progression of esophageal carcinoma by loss of EGF-STAT1 pathway

      CANCER JOURNAL
    92. Anderson, KC
      Novel biologically based therapies for myeloma

      CANCER JOURNAL
    93. Bassett, D; Elbon-Copp, C; Otterbein, S; Barraclough-Mitchell, H; DeLorme, M; Yang, H
      Inflammatory cell availability affects ozone-induced lung damage

      JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
    94. Ding, WX; Shen, HM; Ong, CN
      Critical role of reactive oxygen species formation in microcystin-induced cytoskeleton disruption in primary cultured hepatocytes

      JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
    95. Karrow, NA; Bols, NC; Whyte, JJ; Solomon, KR; Dixon, DG; Boermans, HJ
      Effects of creosote exposure on rainbow trout pronephros phagocyte activity and the percentage of lymphoid B cells

      JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
    96. Abu-Qare, AW; Abou-Donia, MB
      Combined exposure to DEET (N,N-diethyl-m-toluamide) and permethrin-inducedrelease of rat brain mitochondrial cytochrome c

      JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
    97. Kamendulis, LM; Kolaja, KL; Stevenson, DE; Walborg, EF; Klaunig, JE
      Comparative effects of dieldrin on hepatic ploidy, cell proliferation, andapoptosis in rodent liver

      JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
    98. Neuhaus, P; Klupp, J; Langrehr, JM
      mTOR inhibitors: An overview

      LIVER TRANSPLANTATION
    99. Kren, BT; Rodrigues, CMP; Setchell, KDR; Steer, CJ
      Modulation of steady-state messenger RNA levels in the regenerating rat liver with bile acid feeding

      LIVER TRANSPLANTATION
    100. Bishop, GA; McCaughan, GW
      Immune activation is required for the induction of liver allograft tolerance: Implications for immunosuppressive therapy

      LIVER TRANSPLANTATION


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Documento generato il 22/02/20 alle ore 21:21:50