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La ricerca find articoli where soggetti phrase all words 'TISSUE-CULTURE' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 3143 riferimenti
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    1. Hagio, T
      Adventitious shoot regeneration from immature embryos of sorghum

      PLANT CELL TISSUE AND ORGAN CULTURE
    2. Witte, CP; Tiller, SA; Taylor, MA; Davies, HV
      Addition of nickel to Murashige and Skoog medium in plant tissue culture activates urease and may reduce metabolic stress

      PLANT CELL TISSUE AND ORGAN CULTURE
    3. Wyrc, RM; Downes, S
      The role of protein adsorption on chondrocyte adhesion to a heterocyclic methacrylate polymer system

      BIOMATERIALS
    4. Ishikawa, K; Miyamoto, Y; Yuasa, T; Ito, A; Nagayama, M; Suzuki, K
      Fabrication of Zn containing apatite cement and its initial evaluation using human osteoblastic cells

      BIOMATERIALS
    5. Grottkau, BE; Noordin, S; Shortkroff, S; Schaffer, JL; Thornhill, TS; Spector, M
      Effect of mechanical perturbation on the release of PGE(2) by macrophages in vitro

      JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
    6. Xu, FD; Zhang, Z; Frazier, DT
      Microinjection of acetazolamide into the fastigial nucleus augments respiratory output in the rat

      JOURNAL OF APPLIED PHYSIOLOGY
    7. Liu, THA; Stephens, LC; Hannapel, DJ
      Transgenic Solanum brevidens

      BIOTECHNOLOGY IN AGRICULTURE AND FORESTRY 48
    8. Ikeda, K; Nakayashiki, H; Takagi, M; Tosa, Y; Mayama, S
      Heat shock, copper sulfate and oxidative stress activate the retrotransposon MAGGY resident in the plant pathogenic fungus Magnaporthe grisea

      MOLECULAR GENETICS AND GENOMICS
    9. Yamazaki, M; Tsugawa, H; Miyao, A; Yano, M; Wu, J; Yamamoto, S; Matsumoto, T; Sasaki, T; Hirochika, H
      The rice retrotransposon Tos17 prefers low-copy-number sequences as integration targets

      MOLECULAR GENETICS AND GENOMICS
    10. Courtial, B; Feuerbach, F; Eberhard, S; Rohmer, L; Chiapello, H; Camilleri, C; Lucas, H
      Tnt1 transposition events are induced by in vitro transformation of Arabidopsis thaliana, and transposed copies integrate into genes

      MOLECULAR GENETICS AND GENOMICS
    11. Galane, PM; Le Roux, M
      Molecular epidemiology of Escherichia coli isolated from young South African children with diarrhoeal diseases

      JOURNAL OF HEALTH POPULATION AND NUTRITION
    12. Azuma, Y; Shinohara, M; Wang, PL; Ohura, K
      Quinolones alter defense reactions mediated by macrophages

      INTERNATIONAL IMMUNOPHARMACOLOGY
    13. Woller, M; Tessmer, S; Neff, D; Nguema, AA; Van Roo, B; Waechter-Brulla, D
      Leptin stimulates gonadotropin releasing hormone release from cultured intact hemihypothalami and enzymatically dispersed neurons

      EXPERIMENTAL BIOLOGY AND MEDICINE
    14. Schick, B; Kronsbein, H; Kahle, G; Prescher, A; Draf, W
      Papillary tumor of the temporal bone

      SKULL BASE-AN INTERDISCIPLINARY APPROACH
    15. Kim, D; Somji, S; Garrett, SH; Sens, MA; Shukla, D; Sens, DA
      Expression of hsp 27, hsp 60, hsc 70, and hsp 70 by immortalized human proximal tubule cells (HK-2) following exposure to heat shock, sodium arsenite, or cadmium chloride

      JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
    16. Kanzaki, N; Onuma, K; Treboux, G; Tsutsumi, S; Ito, A
      Effect of impurity on two-dimensional nucleation kinetics: Case studies ofmagnesium and zinc on hydroxyapatite (0001) face

      JOURNAL OF PHYSICAL CHEMISTRY B
    17. Coffino, P
      Regulation of cellular polyamines by antizyme

      NATURE REVIEWS MOLECULAR CELL BIOLOGY
    18. Silani, V; Braga, M; Botturi, A; Cardin, V; Bez, A; Pizzuti, A; Scarlato, G
      Human developing motor neurons as a tool to study ALS

      AMYOTROPHIC LATERAL SCLEROSIS AND OTHER MOTOR NEURON DISORDERS
    19. Daniell, SJ; Takahashi, N; Wilson, R; Friedberg, D; Rosenshine, I; Booy, FP; Shaw, RK; Knutton, S; Frankel, G; Aizawa, S
      The filamentous type III secretion translocon of enteropathogenic Escherichia coli

      CELLULAR MICROBIOLOGY
    20. Palonen, P; Linden, L
      Winter hardiness of micropropagated and conventionally propagated strawberry plants

      JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
    21. Ipekci, Z; Altinkut, A; Kazan, K; Bajrovic, K; Gozukirmizi, N
      High frequency plant regeneration from nodal explants of Paulownia elongata

      PLANT BIOLOGY
    22. Katoh, M; Nakada, K; Miyazaki, JI
      Liver regeneration an chicken chorioallantoic membrane

      CELLS TISSUES ORGANS
    23. Kramer, B; Penny, C
      Regulation of embryonic chick insulin cells: Effect of retinoic acid and insulin-like growth factor 1

      CELLS TISSUES ORGANS
    24. Kokubo, T; Ambe-Ono, Y; Nakamura, M; Ishida, H; Yamakawa, T; Kodama, T
      Promotive effect of auxins on UDP-glucose: Flavonol glucosyltransferase activity in Vitis sp cell cultures

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    25. Hirochika, H
      Contribution of the Tos17 retrotransposon to rice functional genomics

      CURRENT OPINION IN PLANT BIOLOGY
    26. Kumar, A; Hirochika, H
      Applications of retrotransposons as genetic tools in plant biology

      TRENDS IN PLANT SCIENCE
    27. Okamoto, H; Hirochika, H
      Silencing of transposable elements in plants

      TRENDS IN PLANT SCIENCE
    28. Carvey, PM; McGuire, SO; Ling, ZD
      Neuroprotective effects of D3 dopamine receptor agonists

      PARKINSONISM & RELATED DISORDERS
    29. Ruhl, A; Trotter, J; Stremmel, W
      Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat

      NEUROGASTROENTEROLOGY AND MOTILITY
    30. Mzouri, K; Amssa, M; Bouiamrine, EH
      Somatic embryogenesis from immature embryos of wheat cultivars (Triticum aestivum L.): genotype effect

      ACTA BOTANICA GALLICA
    31. Mene, P
      Mesangial cell cultures

      JOURNAL OF NEPHROLOGY
    32. Ma, ZJ; Misawa, H; Yamaguchi, M
      Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-beta 1 production with bone growth of newborn rats

      INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
    33. Kodama, M; Kodama, T
      A new trend of breast cancer research in the genome era (Review)

      INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
    34. Ma, ZJ; Yamaguchi, M
      Stimulatory effect of zinc and growth factor on bone protein component in newborn rats: Enhancement with zinc and insulin-like growth factor-I

      INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
    35. Al-Abassi, AA; Farghaly, MM; Ahmed, HL; Mobasher, LLA; Al-Manee, MS
      Infection after laparoscopic cholecystectomy: Effect of infected bile and infected gallbladder wall

      EUROPEAN JOURNAL OF SURGERY
    36. Yee, S; Stevens, B; Coleman, S; Seabrook, JEA; Li, XQ
      High-efficiency regneration in vitro from potato petioles with intact leaflets

      AMERICAN JOURNAL OF POTATO RESEARCH
    37. Watabe, K; Ida, H; Uehara, K; Oyanagi, K; Sakamoto, T; Tanaka, J; Garver, WS; Miyawaki, S; Ohno, K; Eto, Y
      Establishment and characterization of immortalized Schwann cells from murine model of Niemann-Pick disease type C (spm/spm)

      JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM
    38. Lange, N; Vaucher, L; Marti, A; Etter, AL; Gerber, P; van den Bergh, H; Jichlinski, P; Kucera, P
      Routine experimental system for defining conditions used in photodynamic therapy and fluorescence photodetection of (non-) neoplastic epithelia

      JOURNAL OF BIOMEDICAL OPTICS
    39. Ehmann, UK; Calderwood, SK; Stevenson, MA
      Gap-junctional communication between feeder cells and recipient normal epithelial cells correlates with growth stimulation

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
    40. Selznick, SH; Thatcher, ML; Brown, KS; Haussler, CA
      Development and application of computer software for cell culture laboratory management

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
    41. Kwee, S; Raskmark, P; Velizarov, S
      Changes in cellular proteins due to environmental non-ionizing radiation. I. Heat-shock proteins

      ELECTRO- AND MAGNETOBIOLOGY
    42. Van Dolah, FM; Ramsdell, JS
      Review and assessment of in vitro detection methods for algal toxins

      JOURNAL OF AOAC INTERNATIONAL
    43. Snow, DM; Mullins, N; Hynds, DL
      Nervous system-derived chondroitin sulfate proteoglycans regulate growth cone morphology and inhibit neurite outgrowth: A light, epifluorescence, andelectron microscopy study

      MICROSCOPY RESEARCH AND TECHNIQUE
    44. Broadway, DC; Chang, LP
      Trabeculectomy, risk factors for failure and the preoperative state of theconjunctiva

      JOURNAL OF GLAUCOMA
    45. Breto, MP; Ruiz, C; Pina, JA; Asins, MJ
      The diversification of Citrus clementina hort. ex tan., a vegetatively propagated crop species

      MOLECULAR PHYLOGENETICS AND EVOLUTION
    46. Maqbool, SB; Devi, P; Sticklen, MB
      Biotechnology: Genetic improvement of sorghum (Sorghum bicolor (L.) Moench)

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    47. Tyankova, ND; Zagorska, NA
      Genetic control of in vitro response in wheat (Triticum aestivum L.)

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    48. Chakravarty, T; Norcini, JG; Aldrich, JH; Kalmbacher, RS
      Plant regeneration of creeping bluestem (Schizachyrium scoparium (Michx.) Nash var. Stoloniferum (Nash) J. Wipff) via somatic embryogenesis

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    49. Afza, R; Xie, JH; Shen, M; Zapata-Arias, FJ; Fundi, HK; Lee, KS; Bobadilla-Mucino, E; Kodym, A
      Detection of androclonal variation in anther-cultured rice lines using RAPDS

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    50. Chengalrayan, K; Gallo-Meagher, M
      Effect of various growth regulators on shoot regeneration of sugarcane

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    51. Al-Khayri, JM
      Optimization of biotin and thiamine requirements for somatic embryogenesisof date palm (Phoenix dactylifera L.)

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    52. Misra, P; Datta, SK
      Direct differentiation of shoot buds in leaf segments of white marigold (Tagetes erecta L.)

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    53. Ward, KA; Jordan, MC
      Callus formation and plant regeneration from immature and mature embryos of rye (Secale cereale L.)

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    54. Rosas, MM; De La Rosa, MAM; Goldammer, KM; Avila, VMC
      Micropropagation of Turbinicarpus laui glass et Foster, an endemic and endangered species

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    55. Armstrong, G; Johnson, K
      Micropropagation of Ceratopetalum gummiferum, an important Australian cut flower crop

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    56. Aguado-Santacruz, GA; Cabrera-Ponce, JL; Olalde-Portugal, V; Sanchez-Gonzalez, MR; Marquez-Guzman, J; Herrera-Estrella, L
      Tissue culture and plant regeneration of blue grama grass, Bouteloua gracilis (HBK) Lag. ex Steud.

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    57. Oropeza, M; Marcano, AK; De Garcia, E
      Proteins related with embryogenic potential in callus and cell suspensionsof sugarcane (Saccharum sp.)

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    58. Varshney, A; Lakshmikumaran, M; Srivastava, PS; Dhawan, V
      Establishment of genetic fidelity of in vitro-raised Lilium bulblets through RAPD markers

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    59. Kodym, A; Hollenthoner, S; Zapata-Arias, FJ
      Cost reduction in the micropropagation of banana by using tubular skylights as source for natural lighting

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    60. Debnath, SC; McRae, KB
      An efficient in vitro shoot propagation of cranberry (Vaccinium macrocarpon Ait.) by axillary bud proliferation

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    61. Chaturvedi, R; Bhatnagar, SP
      High-frequency shoot regeneration from cotyledon explants of watermelon cv. Sugar Baby

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    62. Compton, ME; Koch, JM
      Influence of plant preservative mixture (PPM)(TM) on adventitious organogenesis in melon, petunia, and tobacco

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    63. Torres, AC; Ze, NM; Cantliffe, DJ
      Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    64. Meurer, CA; Dinkins, RD; Redmond, CT; McAllister, KP; Tucker, DT; Walker, DR; Parrott, WA; Trick, HN; Essig, JS; Frantz, HM; Finer, JJ; Collins, GB
      Embryogenic response of multiple soybean [Glycine max (L.) Merr.] cultivars across three locations

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
    65. Liu, F; Cui, XQ; Horner, HT; Weiner, H; Schnable, PS
      Mitochondrial aldehyde dehydrogenase activity is required for male fertility in maize

      PLANT CELL
    66. Shevchuk, TV; Zakharchenko, NS; Dyachenko, OV; Buryanov, YI
      The effect of the gene encoding EcoRII DNA methyltransferase on the phenotype of transgenic tumor cell lines of Nicotiana tabacum and the methylationlevel of CpNpG sequences in their genome

      RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
    67. Kwon, YS; Eun, MY; Sohn, JK
      Marker-assisted selection for identification of plant regeneration abilityof seed-derived calli in rice (Oryza sativa L.)

      MOLECULES AND CELLS
    68. Kwon, YS; Kim, KM; Eun, MY; Sohn, JK
      Quantitative trait loci mapping associated with plant regeneration abilityfrom seed derived calli in rice (Oryza sativa L.)

      MOLECULES AND CELLS
    69. Feito, I; Gonzalez, A; Centeno, ML; Fernandez, B; Rodriguez, A
      Transport and distribution of benzyladenine in Actinidia deliciosa explants cultured in liquid and solid media

      PLANT PHYSIOLOGY AND BIOCHEMISTRY
    70. Abenavoli, MR; Sorgona, A; Muscolo, A
      Morphological changes in tissue culture of Petunia hybrida in response to coumarin allelochemical

      ALLELOPATHY JOURNAL
    71. Nagata, T
      Three-dimensional high voltage electron microscopy of thick biological specimens

      MICRON
    72. Hamid, M; McCluskey, JT; McClenaghan, NH; Flatt, PR
      Comparative functional study of clonal insulin-secreting cells cultured infive commercially available tissue culture media

      CELL TRANSPLANTATION
    73. Takeda, S; Sugimoto, K; Kakutani, T; Hirochika, H
      Linear DNA intermediates of the Tto1 retrotransposon in Gag particles accumulated in stressed tobacco and Arabidopsis thaliana

      PLANT JOURNAL
    74. Melayah, D; Bonnivard, E; Chalhoub, B; Audeon, C; Grandbastien, MA
      The mobility of the tobacco Tnt1 retrotransposon correlates with its transcriptional activation by fungal factors

      PLANT JOURNAL
    75. Machaalani, R; Lazzaro, V; Duggin, GG
      The characterisation and uptake of paraquat in cultured baboon kidney proximal tubule cells (bPTC)

      HUMAN & EXPERIMENTAL TOXICOLOGY
    76. Henning, T; Brischwein, M; Baumann, W; Ehret, R; Freund, I; Kammerer, R; Lehmann, M; Schwinde, A; Wolf, B
      Approach to a multiparametric sensor-chip-based tumor chemosensitivity assay

      ANTI-CANCER DRUGS
    77. Chung, ES; Joe, EH; Ryu, JK; Kim, J; Lee, YB; Cho, KG; Oh, YJ; Maeng, SH; Baik, HH; Kim, SU; Jin, BK
      GT1b ganglioside induces death of dopaminergic neurons in rat mesencephalic cultures

      NEUROREPORT
    78. Bilak, MM; Kuncl, RW
      Delayed application of IGF-1 and GDNF can rescue already injured postnatalmotor neurons

      NEUROREPORT
    79. Lewis, JS; Thomas, TJ; Klinge, CM; Gallo, MA; Thomas, T
      Regulation of cell cycle and cyclins by 16 alpha-hydroxyestrone in MCF-7 breast cancer cells

      JOURNAL OF MOLECULAR ENDOCRINOLOGY
    80. Sibley, K; Cuthbert-Heavens, D; Knowles, MA
      Loss of heterozygosity at 4p16.3 and mutation of FGFR3 in transitional cell carcinoma

      ONCOGENE
    81. Goldberg, MD; Johnson, M; Hinton, JCD; Williams, PH
      Role of the nucleoid-associated protein Fis in the regulation of virulenceproperties of enteropathogenic Escherichia coli

      MOLECULAR MICROBIOLOGY
    82. Molinari, G; Rohde, M; Talay, SR; Chhatwal, GS; Beckert, S; Podbielski, A
      The role played by the group A streptococcal negative regulator Nra on bacterial interactions with epithelial cells

      MOLECULAR MICROBIOLOGY
    83. Hartung, T; Gstraunthaler, G; Coecke, S; Lewis, D; Blanck, O; Balls, M
      Good Cell Culture Practice (GCCP) - an initiative for standardisation and quality assurance of in vitro studies. The establishment of an ECVAM Task Force on GCCP

      ALTEX-ALTERNATIVEN ZU TIEREXPERIMENTEN
    84. Cantet, S; Fanjul, M; Bremont, F; Midy, V; Hollande, E
      Cytological characterization of apatitic calcium phosphate structures in bronchial epithelial tissue cultured from a child with cystic fibrosis (Delta F508)

      VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY
    85. Jackson, PF; Slusher, BS
      Design of NAALADase inhibitors: A novel neuroprotective strategy

      CURRENT MEDICINAL CHEMISTRY
    86. Dierig, DA; Ray, DT; Coffelt, TA; Nakayama, FS; Leake, GS; Lorenz, G
      Heritability of height, width, resin, rubber, and latex in guayule (Parthenium argentatum)

      INDUSTRIAL CROPS AND PRODUCTS
    87. Ives, TJ; Marston, EL; Regnery, RL; Butts, JD
      In vitro susceptibilities of Bartonella and Rickettsia spp. to fluoroquinolone antibiotics as determined by immunofluorescent antibody analysis of infected Vero cell monolayers

      INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
    88. Penteado, AS; Aidar, L; de Castro, AFP; Yamada, A; Andrade, JRC; Blanco, J; Blanco, M; Blanco, JE
      eae-negative attaching and effacing Escherichia coli from piglets with diarrhea

      RESEARCH IN MICROBIOLOGY
    89. Morito, K; Hirose, T; Kinjo, J; Hirakawa, T; Okawa, M; Nohara, T; Ogawa, S; Inoue, S; Muramatsu, M; Masamune, Y
      Interaction of phytoestrogens with estrogen receptors alpha and beta

      BIOLOGICAL & PHARMACEUTICAL BULLETIN
    90. Sagare, AP; Kuo, CL; Chueh, FS; Tsay, HS
      De novo regeneration of Scrophularia yoshimurae YAMAZAKI (Scrophulariaceae) and quantitative analysis of harpagoside, an iridoid glucoside, formed inaerial and underground parts of in vitro propagated and wild plants by HPLC

      BIOLOGICAL & PHARMACEUTICAL BULLETIN
    91. Yoshida, H; Teramoto, T; Ikeda, K; Yamori, Y
      Glycitein effect on suppressing the proliferation and stimulating the differentiation of osteoblastic MC3T3-E1 cells

      BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
    92. Furuya, K; Fukui, D; Yamaguchi, M; Nakaoka, Y; Bando, G; Kosuge, M
      Isolation of Encephalitozoon cuniculi using primary tissue culture techniques from a rabbit in a colony showing encephalitozoonosis

      JOURNAL OF VETERINARY MEDICAL SCIENCE
    93. Dai, XD; Qu, PM; Dreyfus, CF
      Neuronal signals regulate neurotrophin expression in oligodendrocytes of the basal forebrain

      GLIA
    94. Stence, N; Waite, M; Dailey, ME
      Dynamics of microglial activation: A confocal time-lapse analysis in hippocampal slices

      GLIA
    95. Sevilla, M; Burris, RH; Gunapala, N; Kennedy, C
      Comparison of benefit to sugarcane plant growth and N-15(2) incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and Nif(-) mutant strains

      MOLECULAR PLANT-MICROBE INTERACTIONS
    96. Snow, DM; Smith, JD; Booze, RM; Welch, MA; Mactutus, CF
      Cocaine decreases cell survival and inhibits neurite extension of rat locus coeruleus neurons

      NEUROTOXICOLOGY AND TERATOLOGY
    97. Etienne, H; Bertrand, B
      Trueness-to-type and agronomic characteristics of Coffea arabica trees micropropagated by the embryogenic cell suspension technique

      TREE PHYSIOLOGY
    98. Encina, CL; Constantin, M; Botella, J
      An easy and reliable method for establishment and maintenance of leaf and root cell cultures of Arabidopsis thaliana

      PLANT MOLECULAR BIOLOGY REPORTER
    99. Wang, ZY; Hopkins, A; Mian, R
      Forage and turf grass biotechnology

      CRITICAL REVIEWS IN PLANT SCIENCES
    100. Giri, A; Dhingra, V; Giri, CC; Singh, A; Ward, OP; Narasu, ML
      Biotransformations using plant cells, organ cultures and enzyme systems: current trends and future prospects

      BIOTECHNOLOGY ADVANCES


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Documento generato il 07/08/20 alle ore 23:53:37