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    1. Kim, M; Geum, D; Khang, I; Park, YM; Kang, BM; Lee, KA; Kim, K
      Expression pattern of HSP25 in mouse preimplantation embryo: Heat shock responses during oocyte maturation

      MOLECULAR REPRODUCTION AND DEVELOPMENT
    2. Tanabe, K; Kozawa, O; Niwa, M; Yamomoto, T; Matsuno, H; Ito, H; Kato, K; Dohi, S; Uematsu, T
      Contrasting effects of midazolam on induction of heat shock protein 27 by vasopressin and heat in aortic smooth muscle cells

      JOURNAL OF CELLULAR BIOCHEMISTRY
    3. Kumaraguru, U; Gierynska, M; Norman, S; Bruce, BD; Rouse, BT
      Immunization with chaperone-peptide complex induces low-avidity cytotoxic T lymphocytes providing transient protection against herpes simplex virus infection

      JOURNAL OF VIROLOGY
    4. Hu, JM; Toft, D; Anselmo, D; Wang, XT
      In vitro reconstitution of functional hepadnavirus reverse transcriptase with cellular chaperone proteins

      JOURNAL OF VIROLOGY
    5. Komalavilas, P; Mehta, S; Wingard, CJ; Dransfield, DT; Bhalla, J; Woodrum, JE; Molinaro, JR; Brophy, CM
      PI3-kinase/Akt modulates vascular smooth muscle tone via cAMP signaling pathways

      JOURNAL OF APPLIED PHYSIOLOGY
    6. Fannon, SA; Vidaver, RM; Marts, SA
      Genome and hormones: Gender differences in physiology - Historical perspectives: An abridged history of sex steroid hormone receptor action

      JOURNAL OF APPLIED PHYSIOLOGY
    7. Lincoln, TM; Dey, N; Sellak, H
      Signal transduction in smooth muscle - Invited review: cGMP-dependent protein kinase signaling mechanisms in smooth muscle: from the regulation of tone to gene expression

      JOURNAL OF APPLIED PHYSIOLOGY
    8. Rembold, CM; O'Connor, M; Clarkson, M; Wardle, RL; Murphy, RA
      Signal transduction in smooth muscle - Selected contribution: HSP20 phosphorylation in nitroglycerin- and forskolin-induced sustained reductions in swine carotid media tone

      JOURNAL OF APPLIED PHYSIOLOGY
    9. Cho, JS; Chang, MS; Rho, HM
      Transcriptional activation of the human Cu/Zn superoxide dismutase gene by2,3,7,8-tetrachlorodibenzo-p-dioxin through the xenobiotic-responsive element

      MOLECULAR GENETICS AND GENOMICS
    10. Champion, KM; Nishihara, JC; Joly, JC; Arnott, D
      Similarity of the Escherichia coli proteome upon completion of different biopharmaceutical fermentation processes

      PROTEOMICS
    11. Yan, JX; Harry, RA; Wait, R; Welson, SY; Emery, PW; Preedy, VR; Dunn, MJ
      Separation and identification of rat skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry

      PROTEOMICS
    12. Naumann, A; Hempel, JM; Schorn, K
      Humoral immune response to inner ear proteins in patients with sensorineural hearing loss.

      LARYNGO-RHINO-OTOLOGIE
    13. Biagi, P; Castro, R; Campanella, G; Parisi, G; Gobbini, AR; Vedovini, G
      Takayasu's arteritis and Crohn's disease: an unusual association. Report on two cases

      DIGESTIVE AND LIVER DISEASE
    14. Rahman, MU; Poe, DS; Choi, HK
      Etanercept therapy for immune-mediated cochleovestibular disorders: Preliminary results in a pilot study

      OTOLOGY & NEUROTOLOGY
    15. Cooke, A; Phillips, JM; Parish, NM
      Tolerogenic strategies to halt or prevent type I diabetes

      NATURE IMMUNOLOGY
    16. Das, PK; van den Wijngaard, RMJGJ; Wankowicz-Kalinska, A; Le Poole, IC
      A symbiotic concept of autoimmunity and tumour immunity: lessons from vitiligo

      TRENDS IN IMMUNOLOGY
    17. Wick, G; Perschinka, H; Millonig, G
      Atherosclerosis as an autoimmune disease: an update

      TRENDS IN IMMUNOLOGY
    18. Montecucco, C; Rappuoli, R
      Living dangerously: How Helicobacter pylori survives in the human stomach

      NATURE REVIEWS MOLECULAR CELL BIOLOGY
    19. Rhind, SM; Rae, MT; Brooks, AN
      Effects of nutrition and environmental factors on the fetal programming ofthe reproductive axis

      REPRODUCTION
    20. Mascarenhas, J; Weber, MHW; Graumann, PL
      Specific polar localization of ribosomes in Bacillus subtilis depends on active transcription

      EMBO REPORTS
    21. Agafonov, DE; Kolb, VA; Spirin, AS
      Ribosome-associated protein that inhibits translation at the aminoacyl-tRNA binding stage

      EMBO REPORTS
    22. Hohfeld, J; Cyr, DM; Patterson, C
      From the cradle to the grave: molecular chaperones that may choose betweenfolding and degradation

      EMBO REPORTS
    23. Wilson, RW; Steingrube, VA; Bottger, EC; Springer, B; Brown-Elliott, BA; Vincent, V; Jost, KC; Zhang, YS; Garcia, MJ; Chiu, SH; Onyi, GO; Rossmoore, H; Nash, DR; Wallace, RJ
      Mycobacterium immunogenum sp nov., a novel species related to Mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy

      INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
    24. Yu, XJ; Zhang, XF; McBride, JW; Zhang, YL; Walker, DH
      Phylogenetic relationships of Anaplasma marginale and 'Ehrlichia platys' to other Ehrlichia species determined by GroEL amino acid sequences

      INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
    25. Schett, G; Tohidast-Akrad, M; Steiner, G; Smolen, J
      The stressed synovium

      ARTHRITIS RESEARCH
    26. Ravagnan, L; Gurbuxani, S; Susin, SA; Maisse, C; Daugas, E; Zamzami, N; Mak, T; Jaattela, M; Penninger, JM; Garrido, C; Kroemer, G
      Heat-shock protein 70 antagonizes apoptosis-inducing factor

      NATURE CELL BIOLOGY
    27. Randow, F; Seed, B
      Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability

      NATURE CELL BIOLOGY
    28. Meacham, GC; Patterson, C; Zhang, WY; Younger, JM; Cyr, DM
      The Hsc70 co-chaperone CHIP targets immature CFTR for proteasomal degradation

      NATURE CELL BIOLOGY
    29. Krimmer, T; Geissler, A; Pfanner, N; Rassow, J
      Sorting of preproteins into mitochondria

      CHEMBIOCHEM
    30. Klein, G; Laskowska, E; Taylor, A; Lipinska, B
      IbpA/B small heat-shock protein of marine bacterium Vibrio harveyi binds to proteins aggregated in a cell during heat shock

      MARINE BIOTECHNOLOGY
    31. Rosenhagen, MC; Young, JC; Wochnik, GM; Herr, AS; Schmidt, U; Hartl, FU; Holsboer, F; Rein, T
      Synergistic inhibition of the glucocorticoid receptor by radicicol and benzoquinone ansamycins

      BIOLOGICAL CHEMISTRY
    32. Martin, W; Hoffmeister, M; Rotte, C; Henze, K
      An overview of endosymbiotic models for the origins of eukaryotes, their ATP-producing organelles (mitochondria and hydrogenosomes), and their heterotrophic lifestyle

      BIOLOGICAL CHEMISTRY
    33. Kumar, S; Nussinov, R
      How do thermophilic proteins deal with heat?

      CELLULAR AND MOLECULAR LIFE SCIENCES
    34. Berwin, B; Nicchitta, CV
      To find the road traveled to tumor immunity: The trafficking itineraries of molecular chaperones in antigen-presenting cells

      TRAFFIC
    35. Kabir, MM; Shimizu, K
      Proteome analysis of a temperature-inducible recombinant Escherichia coli for poly-beta-hydroxybutyrate production

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    36. Usui, K; Yoshida, T; Maruyama, T; Yohda, M
      Small heat shock protein of a hyperthermophilic archaeum, Thermococcus sp strain KS-1, exists as a spherical 24 mer and its expression is highly induced under heat-stress conditions

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    37. Hyodo, M; Okamoto, K; Shibata, K; Suematsu, N; Isohashi, F
      Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system

      JOURNAL OF CHROMATOGRAPHY B
    38. Chernoff, YO
      Mutation processes at the protein level: is Lamarck back?

      MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
    39. Sakai, K; Oyama, Y; Okada, Y; Akaike, N; Nakata, M; Chikahisa, L
      Tri-n-butyltin delays the cell death induced by hydrogen peroxide in rat thymocytes

      ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
    40. Xing, Z
      The hunt for new tuberculosis vaccines: Anti-TB immunity and rational design of vaccines

      CURRENT PHARMACEUTICAL DESIGN
    41. Kaur, J; Rao, M; Chakravarti, N; Mathur, M; Shukla, NK; Sanwal, BD; Ralhan, R
      Co-expression of colligin and collagen in oral submucous fibrosis: plausible role in pathogenesis

      ORAL ONCOLOGY
    42. Bohring, C; Krause, E; Habermann, B; Krause, W
      Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease

      MOLECULAR HUMAN REPRODUCTION
    43. Pratt, WB; Krishna, P; Olsen, LJ
      Hsp90-binding immunophilins in plants: the protein movers

      TRENDS IN PLANT SCIENCE
    44. Trieb, K; Kotz, R
      Proteins expressed in osteosarcoma and serum levels as prognostic factors

      INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
    45. Uehara, T; Matsuzawa, H; Nishimura, A
      HscA is involved in the dynamics of FtsZ-ring formation in Escherichia coli K12

      GENES TO CELLS
    46. Gutgsell, NS; Del Campo, M; Raychaudhuri, S; Ofengand, J
      A second function for pseudouridine syntheses: A point mutant of RluD unable to form pseudouridines 1911, 1915, and 1917 in Escherichia coli 23S ribosomal RNA restores normal growth to an RluD-minus strain

      RNA-A PUBLICATION OF THE RNA SOCIETY
    47. Zimmer, C; Von Gabain, A; Henics, T
      Analysis of sequence-specific binding of RNA to Hsp70 and its various homologs indicates the involvement of N- and C-terminal interactions

      RNA-A PUBLICATION OF THE RNA SOCIETY
    48. Baginsky, S; Shteiman-Kotler, A; Liveanu, V; Yehudai-Resheff, S; Bellaoui, M; Settlage, RE; Shabanowitz, J; Hunt, DF; Schuster, G; Gruissem, W
      Chloroplast PNPase exists as a homo-multimer enzyme complex that is distinct from the Escherichia coli degradosome

      RNA-A PUBLICATION OF THE RNA SOCIETY
    49. Bouhouche-Chatelier, H; Chadli, A; Catelli, MG
      The N-terminal adenosine triphosphate binding domain of Hsp90 is necessaryand sufficient for interaction with estrogen receptor

      CELL STRESS & CHAPERONES
    50. Luft, JC; Benjamin, IJ; Mestril, R; Dix, DJ
      Heat shock factor 1-mediated thermotolerance prevents cell death and results in G(2)/M cell cycle arrest

      CELL STRESS & CHAPERONES
    51. Multhoff, G; Pfister, K; Gehrmann, M; Hantschel, M; Gross, C; Hafner, M; Hiddemann, W
      A 14-mer Hsp70 peptide stimulates natural killer (NK) cell activity

      CELL STRESS & CHAPERONES
    52. Kokke, BPA; Boelens, WC; de Jong, WW
      The lack of chaperonelike activity of Caenorhabditis elegans Hsp12.2 cannot be restored by domain swapping with human alpha B-crystallin

      CELL STRESS & CHAPERONES
    53. Walsh, RC; Koukoulas, I; Garnham, A; Moseley, PL; Hargreaves, M; Febbraio, MA
      Exercise increases serum Hsp72 in humans

      CELL STRESS & CHAPERONES
    54. Wu, TC; Ma, JX; Chen, S; Sun, YH; Xiao, CF; Gao, YJ; Wang, RB; Poudrier, J; Dargis, M; Currie, RW; Tanguay, RM
      Association of plasma antibodies against the inducible Hsp70 with hypertension and harsh working conditions

      CELL STRESS & CHAPERONES
    55. Lin, BL; Wang, JS; Liu, HC; Chen, RW; Meyer, Y; Barakat, A; Delseny, M
      Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana

      CELL STRESS & CHAPERONES
    56. Scharf, KD; Siddique, M; Vierling, E
      The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing alpha-crystallin domains (Acd proteins)

      CELL STRESS & CHAPERONES
    57. Krishna, P; Gloor, G
      The Hsp90 family of proteins in Arabidopsis thaliana

      CELL STRESS & CHAPERONES
    58. Lepore, DA; Knight, KR; Anderson, RL; Morrison, WA
      Role of priming stresses and Hsp70 in protection from ischemia reperfusioninjury in cardiac and skeletal muscle

      CELL STRESS & CHAPERONES
    59. Clark, PR; Menoret, A
      The inducible Hsp70 as a marker of tumor immunogenicity

      CELL STRESS & CHAPERONES
    60. Kato, K; Ito, H; Iwamoto, I; Iida, K; Inaguma, Y
      Protein kinase inhibitors can suppress stress-induced dissociation of Hsp27

      CELL STRESS & CHAPERONES
    61. Martin, CC; Tang, P; Barnardo, G; Krone, PH
      Expression of the chaperonin 10 gene during zebrafish development

      CELL STRESS & CHAPERONES
    62. Kumar, P; Ward, BK; Minchin, RF; Ratajczak, T
      Regulation of the Hsp90-binding immunophilin, cyclophilin 40, is mediated by multiple sites for CA-binding protein (GABP)

      CELL STRESS & CHAPERONES
    63. Heinlein, CA; Chang, CS
      Role of chaperones in nuclear translocation and transactivation of steroidreceptors

      ENDOCRINE
    64. Lacorn, M; Piechotta, G; Simat, TJ; Kammann, U; Wosniok, W; Lang, T; Muller, WEG; Schroder, HC; Jenke, HS; Steinhart, H
      Annual cycles of apoptosis, DNA strand breaks, heat shock proteins, and metallothionen isoforms in dab (Limanda limanda): influences of natural factors and consequences for biological effect monitoring

      BIOMARKERS
    65. Gallucci, S
      Novel activators of dendritic cells as fundamental tools in immunotherapy

      EXPERT OPINION ON THERAPEUTIC PATENTS
    66. de Smet, MD; Chan, CC
      Regulation of ocular inflammation - what experimental and human studies have taught us

      PROGRESS IN RETINAL AND EYE RESEARCH
    67. Ryan, AF; Keithley, EM; Harris, JP
      Autoimmune inner ear disorders

      CURRENT OPINION IN NEUROLOGY
    68. Piper, P; Calderon, CO; Hatzixanthis, K; Mollapour, M
      Weak acid adaptation: the stress response that confers yeasts with resistance to organic acid food preservatives

      MICROBIOLOGY-SGM
    69. Hennequin, C; Porcheray, F; Waligora-Dupriet, AJ; Collignon, A; Bare, MC; Bourlioux, P; Karjalainen, T
      GroEL (Hsp60) of Clostridium difficile is involved in cell adherence

      MICROBIOLOGY-UK
    70. Espinosa, E; Belmant, C; Sicard, H; Poupot, R; Bonneville, M; Fournie, JJ
      Y2K+1 state-of-the-art on non-peptide phosphoantigens, a novel category ofimmunostimulatory molecules

      MICROBES AND INFECTION
    71. Zhang, MX; Hisaeda, H; Kano, S; Matsumoto, Y; Hao, YP; Looaresuwan, S; Aikawa, M; Himeno, K
      Antibodies specific for heat shock proteins in human and murine malaria

      MICROBES AND INFECTION
    72. Takeno, S; Noguchi, T; Takahashi, Y; Kikuchi, R; Uchida, Y; Yokoyama, S
      Immunohistochemical and clinicopathologic analysis of response to neoadjuvant therapy for esophageal squamous cell carcinoma

      DISEASES OF THE ESOPHAGUS
    73. Gebel, S; Muller, T
      The activity of NF-kappa B in Swiss 3T3 cells exposed to aqueous extracts of cigarette smoke is dependent on thioredoxin

      TOXICOLOGICAL SCIENCES
    74. Watanabe, A; Uchida, I; Nakata, K; Fujimoto, Y; Oikawa, S
      Molecular cloning of bovine (Bos taurus) cDNA encoding a 94-kDa glucose-regulated protein and developmental changes in its mRNA and protein content in the mammary gland

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
    75. Sathiyaa, R; Campbell, T; Vijayan, MM
      Cortisol modulates HSP90 mRNA expression in primary cultures of trout hepatocytes

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
    76. Clegg, JS
      Cryptobiosis - a peculiar state of biological organization

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
    77. Fields, PA
      Review: Protein function at thermal extremes: balancing stability and flexibility

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
    78. Dinh, HKB; Zhao, BT; Schuschereba, ST; Merrill, G; Bowman, PD
      Gene expression profiling of the response to thermal injury in human cells

      PHYSIOLOGICAL GENOMICS
    79. Liu, YF; Steinacker, JM
      Changes in skeletal muscle heat shock proteins: Pathological significance

      FRONTIERS IN BIOSCIENCE
    80. Milner, CM; Campbell, RD
      Genetic organization of the human MHC class III region

      FRONTIERS IN BIOSCIENCE
    81. Reitzer, L; Schneider, BL
      Metabolic context and possible physiological themes of sigma(54)-dependentgenes in Escherichia coli

      MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
    82. Miyagi, T; Hori, O; Egawa, M; Kato, H; Kitagawa, Y; Konaka, H; Ozawa, K; Koshida, K; Uchibayashi, T; Ogawa, S; Namiki, M
      Antitumor effect of reduction of 150-kDa oxygen-regulated protein expression in human prostate cancer cells

      MOLECULAR UROLOGY
    83. Yu, Q; Kent, C; Tytell, M
      Retinal uptake of intravitreally injected Hsc/Hsp70 and its effect on susceptibility to light damage

      MOLECULAR VISION
    84. Rucker, N; Ferrario, A; Gomer, CJ
      Constitutive overexpression of HSP-70 in thermal resistant tumor cells does not alter sensitivity to porphyrin-, chlorin-, or purpurin-mediated PDT

      JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
    85. Brodsky, FM; Chen, CY; Knuehl, C; Towler, MC; Wakeham, DE
      Biological basket weaving: Formation and function of clathrin-coated vesicles

      ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
    86. Rocnik, E; Saward, L; Pickering, JG
      HSP47 expression by smooth muscle cells is increased during arterial development and lesion formation and is inhibited by fibrillar collagen

      ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
    87. Hall, DM; Sattler, GL; Sattler, CA; Zhang, HJ; Oberley, LW; Pitot, HC; Kregel, KC
      Aging lowers steady-state antioxidant enzyme and stress protein expressionin primary hepatocytes

      JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
    88. Le Naour, F; Misek, DE; Krause, MC; Deneux, L; Giordano, TJ; Scholl, S; Hanash, SM
      Proteomics-based identification of RS/DJ-1 as a novel circulating tumor antigen in breast cancer

      CLINICAL CANCER RESEARCH
    89. Okopien, B; Hyper, M; Kowalski, J; Belowski, D; Madej, A; Zielinski, M; Tokarz, D; Kalina, Z; Herman, ZS
      A new immunological marker of atherosclerotic injury of arterial wall

      RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY
    90. Hansen, MH; Ostenstad, B; Sioud, M
      Antigen-specific IgG antibodies in stage IV long-time survival breast cancer patients

      MOLECULAR MEDICINE
    91. Adrie, C; Bachelet, M; Vayssier-Taussat, M; Russo-Marie, F; Bouchaert, I; Adib-Conquy, M; Cavaillon, JM; Pinsky, MR; Dhainaut, JF; Polla, BS
      Mitochondrial membrane potential and apoptosis peripheral blood monocytes in severe human sepsis

      AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
    92. Dunn, BE; Grutter, MG
      Helicobacter pylori springs another surprise

      NATURE STRUCTURAL BIOLOGY
    93. Ha, NC; Oh, ST; Sung, JY; Cha, KA; Lee, MH; Oh, BH
      Supramolecular assembly and acid resistance of Helicobacter pylori urease

      NATURE STRUCTURAL BIOLOGY
    94. Brehmer, D; Rudiger, S; Gassler, CS; Klostermeier, D; Packschies, L; Reinstein, J; Mayer, MP; Bukau, B
      Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotideexchange

      NATURE STRUCTURAL BIOLOGY
    95. Kim, SJ; Jeong, DG; Chi, SW; Lee, JS; Ryu, SE
      Crystal structure of proteolytic fragments of the redox-sensitive Hsp33 with constitutive chaperone activity

      NATURE STRUCTURAL BIOLOGY
    96. Ishii, E; Yokota, K; Sugiyama, T; Fujinaga, Y; Ayada, K; Hokari, I; Hayashi, S; Hirai, Y; Asaka, M; Oguma, K
      Immunoglobulin G1 antibody response to Helicobacter pylori heat shock protein 60 is closely associated with low-grade gastric mucosa-associated lymphoid tissue lymphoma

      CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
    97. Takeno, S; Noguchi, T; Kikuchi, R; Sato, T; Uchida, Y; Yokoyama, S
      Analysis of the survival period in resectable stage IV gastric cancer

      ANNALS OF SURGICAL ONCOLOGY
    98. Frankel, J; Williams, NE; Nelsen, EM; Keeling, PJ
      An evaluation of Hsp90 as a mediator of cortical patterning in Tetrahymena

      JOURNAL OF EUKARYOTIC MICROBIOLOGY
    99. Kaarniranta, K; Holmberg, CI; Lammi, MJ; Eriksson, JE; Sistonen, L; Helminen, HJ
      Primary chondrocytes resist hydrostatic pressure-induced stress while primary synovial cells and fibroblasts show modified Hsp70 response

      OSTEOARTHRITIS AND CARTILAGE
    100. Deschesnes, RG; Huot, J; Valerie, K; Landry, J
      Involvement of p38 in apoptosis-associated membrane blebbing and nuclear condensation

      MOLECULAR BIOLOGY OF THE CELL


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Documento generato il 29/10/20 alle ore 13:16:49