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La ricerca find articoli where soggetti phrase all words 'SENSITIVE DETECTION' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 283 riferimenti
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    1. Dickert, FL; Reif, M; Sikorski, R
      Shaping tetraazaparacyclophanes for selective solvent vapour detection: Structural variations in coating design

      JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
    2. Forget, D; Boturyn, D; Defrancq, E; Lhomme, J; Dumy, P
      Highly efficient synthesis of peptide-oligonucleotide conjugates: Chemoselective oxime and thiazolidine formation

      CHEMISTRY-A EUROPEAN JOURNAL
    3. Aerts, J; Wynendaele, W; Paridaens, R; Christiaens, MR; van den Bogaert, W; van Oosterom, AT; Vandekerckhove, F
      A real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to detect breast carcinoma cells in peripheral blood

      ANNALS OF ONCOLOGY
    4. Ylikoski, A; Karp, M; Lilja, H; Lovgren, T
      Dual-label detection of amplified products in quantitative RT-PCR assay using lanthanide-labeled probes

      BIOTECHNIQUES
    5. Holland, WG; Claes, F; My, LN; Thanh, NG; Tam, PT; Verloo, D; Buscher, P; Goddeeris, B; Vercruysse, J
      A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes

      VETERINARY PARASITOLOGY
    6. Bengaly, Z; Kasbari, M; Desquesnes, M; Sidibe, I
      Validation of a polymerase chain reaction assay for monitoring the therapeutic efficacy of diminazene aceturate in trypanosome-infected sheep

      VETERINARY PARASITOLOGY
    7. Hattinger, CM; Rumpler, S; Kovar, H; Ambros, PF
      Fine-mapping of cytogenetically undetectable EWS/ERG fusions on DNA fibersof Ewing tumors

      CYTOGENETICS AND CELL GENETICS
    8. Leone, F; Perissinotto, E; Viale, A; Cavalloni, G; Taraglio, S; Capaldi, A; Piacibello, W; Torchio, B; Aglietta, M
      Detection of breast cancer cell contamination in leukapheresis product by real-time quantitative polymerase chain reaction

      BONE MARROW TRANSPLANTATION
    9. Mole, RJ; Maskell, TWO
      Phage as a diagnostic - the use of phage in TB diagnosis

      JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
    10. Stewart, S; Nassuth, A
      RT-PCR based detection of Rupestris stem pitting associated virus within field-grown grapevines throughout the year

      PLANT DISEASE
    11. Magistrado, PA; Garcia, MM; Raymundo, AK
      Isolation and polymerase chain reaction-based detection of Campylobacter jejuni and Campylobacter coli from poultry in the Philippines

      INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
    12. Ooka, M; Tamaki, Y; Sakita, I; Fujiwara, Y; Yamamoto, H; Miyake, Y; Sekimoto, M; Ohue, M; Sugita, Y; Miyoshi, Y; Ikeda, N; Noguchi, S; Monden, M
      Bone marrow micrometastases detected by RT-PCR for mammaglobin can be an alternative prognostic factor of breast cancer

      BREAST CANCER RESEARCH AND TREATMENT
    13. Xiao, LH; Singh, A; Limor, J; Graczyk, TK; Gradus, S; Lal, A
      Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater

      APPLIED AND ENVIRONMENTAL MICROBIOLOGY
    14. Crissman, HA; Steinkamp, JA
      Flow cytometric fluorescence lifetime measurements

      METHODS IN CELL BIOLOGY, VOL 63
    15. Pogacnik, L; Franko, M
      Optimisation of FIA system for detection of organophosphorus and carbamatepesticides based on cholinesterase inhibition

      TALANTA
    16. Raimond, JM; Brune, M; Haroche, S
      Colloquium: Manipulating quantum entanglement with atoms and photons in a cavity

      REVIEWS OF MODERN PHYSICS
    17. Najera, C; Sanchez, F; Mateu, E; Prieto, F; Beneyto, M
      Early diagnosis of retinoblastoma: usefulness of searching for RB1 gene mutations

      MEDICINA CLINICA
    18. Panagopoulos, I; Mertens, F; Domanski, HA; Isaksson, M; Brosjo, O; Gustafson, P; Mandahl, N
      No EWS/FLI1 fusion transcripts in giant-cell tumors of bone

      INTERNATIONAL JOURNAL OF CANCER
    19. Bellantone, R; Lombardi, CP; Bossola, M; Ferrante, A; Princi, P; Boscherini, M; Maussier, L; Salvatori, M; Rufini, V; Reale, F; Romano, L; Tallini, G; Zelano, G; Pontecorvi, A
      Validity of thyroglobulin mRNA assay in peripheral blood of postoperative thyroid carcinoma patients in predicting tumor recurrences varies accordingto the histologic type - Results of a prospective study

      CANCER
    20. Kasahara, T; Hara, N; Bilim, V; Tomita, Y; Saito, K; Obara, K; Takahashi, K
      Sequential detection of alphafetoprotein-bearing cells in blood stem cell fraction of germ cell tumour patients

      BRITISH JOURNAL OF CANCER
    21. Ogawa, T; Mizutani, M; Inoue, T
      Dependence of the laser two photon ionization process in solution on the laser pulse width

      ANALYTICAL CHEMISTRY
    22. Esch, MB; Baeumner, AJ; Durst, RA
      Detection of Cryptosporidium parvum using oligonucleotide-tagged liposomesin a competitive assay format

      ANALYTICAL CHEMISTRY
    23. Simonian, AL; Grimsley, JK; Flounders, AW; Schoeniger, JS; Cheng, TC; DeFrank, JJ; Wild, JR
      Enzyme-based biosensor for the direct detection of fluorine-containing organophosphates

      ANALYTICA CHIMICA ACTA
    24. Litani-Barzilai, I; Fisher, M; Gridin, VV; Schechter, I
      Fast filter-sampling and analysis of PAH aerosols by laser multiphoton ionization

      ANALYTICA CHIMICA ACTA
    25. Bruun, L; Koch, C; Jakobsen, MH; Pedersen, B; Christiansen, M; Aamand, J
      Characterization of monoclonal antibodies raised against different structures belonging to the s-triazine group of herbicides

      ANALYTICA CHIMICA ACTA
    26. Lee, YM; Johnson, PW; Call, JL; Arrowood, MJ; Furness, BW; Pichette, SC; Grady, KK; Reeh, P; Mitchell, L; Bergmire-Sweat, D; MacKenzie, WR; Tsang, VCW
      Development and application of a quantitative, specific assay for Cryptosporidium parvum oocyst detection in high-turbidity environmental water samples

      AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
    27. Ermert, L; Hocke, AC; Duncker, HR; Seeger, W; Ermert, M
      Comparison of different detection methods in quantitative microdensitometry

      AMERICAN JOURNAL OF PATHOLOGY
    28. Ferlito, A; Partridge, M; Brennan, J; Hamakawa, H
      Lymph node micrometastases in head and neck cancer: A review

      ACTA OTO-LARYNGOLOGICA
    29. Sato, M; Kaieda, T; Ohmukai, K; Kawazumi, H; Harata, A; Ogawa, T
      Acid-base equilibrium constants at the water surface and distribution coefficients between the surface and the bulk as studied by the laser two-photon ionization technique

      JOURNAL OF PHYSICAL CHEMISTRY B
    30. Gorbatchuk, VV; Tsifarkin, AG; Antipin, IS; Solomonov, BN; Konovalov, AI; Seidel, J; Baitalov, F
      Thermodynamic comparison of molecular recognition of vaporous guests by solid calixarene and diol hosts

      JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
    31. Manganaris, S; Christensen, M; Zerkle, D; Hermiz, K
      A data mining analysis of RTID alarms

      COMPUTER NETWORKS-THE INTERNATIONAL JOURNAL OF COMPUTER AND TELECOMMUNICATIONS NETWORKING
    32. Hallier-Soulier, S; Guillot, E
      Detection of cryptosporidia and Cryptosporidium parvum oocysts in environmental water samples by immunomagnetic separation-polymerase chain reaction

      JOURNAL OF APPLIED MICROBIOLOGY
    33. Wang, YL; Einhorn, P; Triche, TJ; Seeger, RC; Reynolds, CP
      Expression of protein gene product 9.5 and tyrosine hydroxylase in childhood small round cell tumors

      CLINICAL CANCER RESEARCH
    34. Gridin, VV; Inoue, T; Ogawa, T; Schechter, I
      On-line screening of airborne PAH contamination by simultaneous multiphoton ionization and laser induced fluorescence

      INSTRUMENTATION SCIENCE & TECHNOLOGY
    35. Thiesse, P; Hany, MA; Combaret, V; Renchere-Vince, D; Bouffet, E; Bergeron, C
      Assessment of percutaneous fine needle aspiration cytology as a technique to provide diagnostic and prognostic information in neuroblastoma

      EUROPEAN JOURNAL OF CANCER
    36. Almeida, PF; Almeida, RCC
      A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes

      FOOD CONTROL
    37. Stefan, RI; van Staden, JF; Aboul-Enein, HY
      Immunosensors in clinical analysis

      FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
    38. Kohler, S; Belkin, S; Schmid, RD
      Reporter gene bioassays in environmental analysis

      FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
    39. Poussier, S; Luisetti, J
      Specific detection of biovars of Ralstonia solanacearum in plant tissues by Nested-PCR-RFLP

      EUROPEAN JOURNAL OF PLANT PATHOLOGY
    40. Fietzek, C; Bodenhofer, K; Hees, M; Haisch, P; Hanack, M; Gopel, W
      Soluble phthalocyanines as suitable coatings for highly sensitive gas phase VOC-detection

      SENSORS AND ACTUATORS B-CHEMICAL
    41. Ogawa, T; Tenkyuu, Y; Nakashima, K
      Two-photon ionization efficiency of pyrene and several aromatic compounds at 240, 266 and 299 nm and its analytical applications

      ANALYTICAL SCIENCES
    42. Sabbatini, R; Federico, M; Morselli, M; Depenni, R; Cagossi, K; Luppi, M; Torelli, G; Silingardi, V
      Detection of circulating tumor cells by reverse transcriptase polymerase chain reaction of maspin in patients with breast cancer undergoing conventional-dose chemotherapy

      JOURNAL OF CLINICAL ONCOLOGY
    43. O'Connor, L; Joy, J; Kane, M; Smith, T; Maher, M
      Rapid polymerase chain reaction/DNA probe membrane-based assay for the detection of Listeria and Listeria monocytogenes in food

      JOURNAL OF FOOD PROTECTION
    44. Wahl, U
      Advances in electron emission channeling measurements in semiconductors

      HYPERFINE INTERACTIONS
    45. Wahl, U
      Advances in electron emission channeling measurements in semiconductors

      HYPERFINE INTERACTIONS
    46. Koo, K; Jaykus, LA
      Selective amplification of bacterial RNA: use of a DNA primer containing mismatched bases near its 3 ' terminus to reduce false-positive signals

      LETTERS IN APPLIED MICROBIOLOGY
    47. Bessho, A; Tabata, M; Kiura, K; Takata, I; Nagata, T; Fujimoto, N; Kunisada, K; Ueoka, H; Harada, M
      Detection of occult tumor cells in peripheral blood from patients with small cell lung cancer by reverse transcriptase-polymerase chain reaction

      ANTICANCER RESEARCH
    48. Jenkins, MC; Trout, J; Abrahamsen, MS; Lancto, CA; Higgins, J; Fayer, R
      Estimating viability of Cryptosporidium parvum oocysts using reverse transcriptase-polymerase chain reaction (RT-PCR) directed at mRNA encoding amyloglucosidase

      JOURNAL OF MICROBIOLOGICAL METHODS
    49. Nassuth, A; Pollari, E; Helmeczy, K; Stewart, S; Kofalvi, SA
      Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts

      JOURNAL OF VIROLOGICAL METHODS
    50. Bidawid, S; Farber, JM; Sattar, SA
      Rapid concentration and detection of hepatitis A virus from lettuce and strawberries

      JOURNAL OF VIROLOGICAL METHODS
    51. von Wintzingerode, F; Landt, O; Ehrlich, A; Gobel, UB
      Peptide nucleic acid-mediated PCR clamping as a useful supplement in the determination of microbial diversity

      APPLIED AND ENVIRONMENTAL MICROBIOLOGY
    52. Xiao, LH; Alderisio, K; Limor, J; Royer, M; Lal, AA
      Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool

      APPLIED AND ENVIRONMENTAL MICROBIOLOGY
    53. Olesen, CEM; Yan, YX; Liu, B; Martin, D; D'Eon, B; Judware, R; Martin, C; Voyta, JC; Bronstein, I
      Novel methods for chemiluminescent detection of reporter enzymes

      APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PT A
    54. Olesen, CEM; Martin, CS; Mosier, J; Liu, B; Voyta, JC; Bronstein, I
      Chemiluminescent reporter gene assays with 1,2-dioxetane enzyme substrates

      BIOLUMINESCENCE AND CHEMILUMINESCENCE, PT C
    55. Wan, K; Chovelon, JM; Jaffrezic-Renault, N
      Enzyme-octadecylamine Langmuir-Blodgett membranes for ENFET biosensors

      TALANTA
    56. Berges, R; Rott, M; Seemuller, E
      Range of phytoplasma concentrations in various plant hosts as determined by competitive polymerase chain reaction

      PHYTOPATHOLOGY
    57. Turner, JJ; Milliken, S
      A case of keratin-positive acute myeloid leukemia: A possible role for cytokeratin 19 as a specific epithelial marker

      PATHOLOGY
    58. Ko, Y; Grunewald, E; Totzke, G; Klinz, M; Fronhoffs, S; Gouni-Berthold, I; Sachinidis, A; Vetter, H
      High percentage of false-positive results of cytokeratin 19 RT-PCR in blood: A model for the analysis of illegitimate gene expression

      ONCOLOGY
    59. Chappell, SA; Johnson, SM; Shaw, JA; Walker, RA
      Expression of oestrogen receptor alpha variants in non-malignant breast and early invasive breast carcinomas

      JOURNAL OF PATHOLOGY
    60. Bolotin, IL; Houssiau, L; Rabalais, JW
      Real-space surface crystallography: Experimental stereographic projectionsfrom ion scattering

      JOURNAL OF CHEMICAL PHYSICS
    61. Blewett, J; Blewett, IJ; Woodward, PK
      Phase and amplitude responses associated with the measurement of shear-wave velocity in sand by bender elements

      CANADIAN GEOTECHNICAL JOURNAL
    62. Bojunga, J; Roddiger, S; Stanisch, M; Kusterer, K; Kurek, R; Renneberg, H; Adams, S; Lindhorst, E; Usadel, KH; Schumm-Draeger, PM
      Molecular detection of thyroglobulin mRNA transcripts in peripheral blood of patients with thyroid disease by RT-PCR

      BRITISH JOURNAL OF CANCER
    63. Lee, YA; Wang, CC
      The design of specific primers for the detection of Ralstonia solanacearumin soil samples by polymerase chain reaction

      BOTANICAL BULLETIN OF ACADEMIA SINICA
    64. Made, D; Stark, R
      Detection of thermophilic campylobacter in food by polymerase chain reaction

      Archiv für Lebensmittelhygiene
    65. Myal, Y; Blanchard, A; Watson, P; Corrin, M; Shiu, R; Iwasiow, B
      Detection of genetic point mutations by peptide nucleic acid-mediated polymerase chain reaction clamping using paraffin-embedded specimens

      ANALYTICAL BIOCHEMISTRY
    66. Maggiolini, M; Donze, O; Picard, D
      A non-radioactive method for inexpensive quantitative RT-PCR

      BIOLOGICAL CHEMISTRY
    67. Waage, AS; Vardund, T; Lund, V; Kapperud, G
      Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay

      JOURNAL OF APPLIED MICROBIOLOGY
    68. Toth, IK; Hyman, LJ; Wood, JR
      A one step PCR-based method for the detection of economically important soft rot Erwinia species on micropropagated potato plants

      JOURNAL OF APPLIED MICROBIOLOGY
    69. Olesen, CEM; Martin, CS; Voyta, JC; Edwards, B; Bronstein, I
      Dioxetane substrates for alkaline phosphatase labels

      JOURNAL OF CLINICAL LIGAND ASSAY
    70. Kopreski, MS; Benko, FA; Kwak, LW; Gocke, CD
      Detection of tumor messenger RNA in the serum of patients with malignant melanoma

      CLINICAL CANCER RESEARCH
    71. Mellado, B; Gutierrez, L; Castel, T; Colomer, D; Fontanillas, M; Castro, J; Estape, J
      Prognostic significance of the detection of circulating malignant cells byreverse transcriptase-polymerase chain reaction in long-term clinically disease-free melanoma patients

      CLINICAL CANCER RESEARCH
    72. Montamat, EE; Durand, S; Bocco, JL; D'Oro, GMD; Blanco, A
      Identification of Trypanosoma cruzi zymodemes by kinetoplast DNA probes

      JOURNAL OF EUKARYOTIC MICROBIOLOGY
    73. Nakajima, K; Micheletto, R; Mitsui, K; Isoshima, T; Hara, M; Wada, T; Sasabe, H; Knoll, W
      Hybridization of scanning near-field optical microscope with scanning tunneling microscope

      MOLECULAR CRYSTALS AND LIQUID CRYSTALS SCIENCE AND TECHNOLOGY SECTION A-MOLECULAR CRYSTALS AND LIQUID CRYSTALS
    74. Hayashi, K
      Recent enhancements in SSCP

      GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING
    75. Zhang, L; Liu, Y; Williams, JAR; Bennion, I
      Enhanced FBG strain sensing multiplexing capacity using combination of intensity and wavelength dual-coding technique

      IEEE PHOTONICS TECHNOLOGY LETTERS
    76. Espinosa, M; Atanasov, P; Wilkins, E
      Development of a disposable organophosphate biosensor

      ELECTROANALYSIS
    77. Mandrell, RE; Wachtel, MR
      Novel detection techniques for human pathogens that contaminate poultry

      CURRENT OPINION IN BIOTECHNOLOGY
    78. Ivanov, EV
      Bandpass crystal filter for enhanced phase-sensitive detection

      MEASUREMENT SCIENCE & TECHNOLOGY
    79. Geymayer, P; Bahr, N; Reymond, JL
      A general fluorogenic assay for catalysis using antibody sensors

      CHEMISTRY-A EUROPEAN JOURNAL
    80. Wojczewski, C; Stolze, K; Engels, JW
      Fluorescent oligonucleotides - Versatile tools as probes and primers for DNA and RNA analysis

      SYNLETT
    81. Boudazin, G; Le Roux, AC; Josi, K; Labarre, P; Jouan, B
      Design of division specific primers of Ralstonia solanacearum and application to the identification of European isolates

      EUROPEAN JOURNAL OF PLANT PATHOLOGY
    82. Dickert, FL; Sikorski, R
      Supramolecular strategies in chemical sensing

      MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
    83. Jaffrezic-Renault, N; Senillou, A; Martelet, C; Wan, K; Chovelon, JM
      ISFET microsensors for the detection of pollutants in liquid media

      SENSORS AND ACTUATORS B-CHEMICAL
    84. Cahill, DM
      Detection, identification and disease diagnosis of soilborne pathogens

      AUSTRALASIAN PLANT PATHOLOGY
    85. Gianfrani, L; Fox, RW; Hollberg, L
      Cavity-enhanced absorption spectroscopy of molecular oxygen

      JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
    86. Tseng, CJ; Pao, CC; Lin, JD; Soong, YK; Hong, JH; Hsueh, S
      Detection of human papillomavirus types 16 and 18 mRNA in peripheral bloodof advanced cervical cancer patients and its association with prognosis

      JOURNAL OF CLINICAL ONCOLOGY
    87. Bryan, PD; Emry, ML; El-Shourbagy, TA
      Determination of ABT-089 in human plasma by high performance liquid chromatography using in situ precolumn derivatization with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole

      JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
    88. Jaffrezic-Renault, N; Wan, K; Senillou, A; Chovelon, JM; Martelet, C
      Development of new polymeric membranes for ENFETs for biomedical and environmental applications

      ANALUSIS
    89. Reymond, JL
      Catalytic antibodies for organic synthesis

      BIOCATALYSIS - FROM DISCOVERY TO APPLICATION
    90. Olmos, A; Cambra, M; Esteban, O; Gorris, MT; Terrada, E
      New device and method for capture, reverse transcription and nested PCR ina single closed-tube

      NUCLEIC ACIDS RESEARCH
    91. Solano, P; Michel, JF; LeFrancois, T; de La Rocque, S; Sidibe, I; Zoungrana, A; Cuisance, D
      Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso

      VETERINARY PARASITOLOGY
    92. Vannucchi, AM; Glinz, S; Bosi, A; Fallai, C; Rossi-Ferrini, P
      Detection of contaminating tumor cells by RT-PCR in breast cancer patients

      BONE MARROW TRANSPLANTATION
    93. Steinkamp, JA; Lehnert, NM; Keij, JF; Lehnert, BE
      Enhanced immunofluorescence measurement resolution of surface antigens on highly autofluorescent, glutaraldehyde-fixed cells analyzed by phase-sensitive flow cytometry

      CYTOMETRY
    94. Nakajima, K; Micheletto, R; Mitsui, K; Isoshima, T; Hara, M; Wada, T; Sasabe, H; Knoll, W
      Nanoscopic studies investigated by hybrid SNOM STM

      APPLIED SURFACE SCIENCE
    95. Llop, P; Caruso, P; Cubero, J; Morente, C; Lopez, MM
      A simple extraction procedure for efficient routine detection of pathogenic bacteria in plant material by polymerase chain reaction

      JOURNAL OF MICROBIOLOGICAL METHODS
    96. Lambrechts, AC; Bosma, AJ; Klaver, SG; Top, B; Perebolte, L; van't Veer, LJ; Rodenhuis, S
      Comparison of immunocytochemistry, reverse transcriptase polymerase chain reaction, and nucleic acid sequence-based amplification for the detection of circulating breast cancer cells

      BREAST CANCER RESEARCH AND TREATMENT
    97. Greening, GE; Woodfield, L; Lewis, GD
      RT-PCR and chemiluminescent ELISA for detection of enteroviruses

      JOURNAL OF VIROLOGICAL METHODS
    98. Foray, S; Pailloud, F; Thouvenot, D; Floret, D; Aymard, M; Lina, B
      Evaluation of combining upper respiratory tract swab samples with cerebrospinal fluid examination for the diagnosis of enteroviral meningitis in children

      JOURNAL OF MEDICAL VIROLOGY
    99. Norton, DM; Batt, CA
      Detection of viable Listeria monocytogenes with a 5 ' nuclease PCR assay

      APPLIED AND ENVIRONMENTAL MICROBIOLOGY
    100. Poussier, S; Vandewalle, P; Luisetti, J
      Genetic diversity of African and worldwide strains of Ralstonia solanacearum as determined by PCR-restriction fragment length polymorphism analysis of the hrp gene region

      APPLIED AND ENVIRONMENTAL MICROBIOLOGY


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Documento generato il 26/01/21 alle ore 23:08:52