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La ricerca find articoli where soggetti phrase all words 'SCRAPIE PRION' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 179 riferimenti
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    1. Soto, C; Saborio, GP
      Prions: disease propagation and disease therapy by conformational transmission

      TRENDS IN MOLECULAR MEDICINE
    2. Vitagliano, V; D'Errico, G
      A simple kinetic model to describe the progression of prion disease

      PHYSICAL CHEMISTRY CHEMICAL PHYSICS
    3. Negro, A; Ballarin, C; Bertoli, A; Massimino, ML; Sorgato, MC
      The metabolism and imaging in live cells of the bovine prion protein in its native form or carrying single amino acid substitutions

      MOLECULAR AND CELLULAR NEUROSCIENCE
    4. Clarke, AR; Jackson, GS; Collinge, J
      The molecular biology of prion propagation

      PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
    5. Levy, Y; Hanan, E; Solomon, B; Becker, OM
      Helix-coil transition of PrP106-126: Molecular dynamic study

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    6. Iovino, M; Falconi, M; Petruzzelli, R; Desideri, A
      Role of the helix capping in the stability of the mouse prion (180-213) segment: Investigation through molecular dynamics simulations.

      JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
    7. Brown, DR
      Copper and prion disease

      BRAIN RESEARCH BULLETIN
    8. Jackson, GS
      Spontaneous conformational change within the prion protein - implications for disease pathogenesis?

      BIOESSAYS
    9. Yedidia, Y; Horonchik, L; Tzaban, S; Yanai, A; Taraboulos, A
      Proteasomes and ubiquitin are involved in the turnover of the wild-type prion protein

      EMBO JOURNAL
    10. Norkin, LC
      Caveolae in the uptake and targeting of infectious agents and secreted toxins

      ADVANCED DRUG DELIVERY REVIEWS
    11. Collinge, J
      Prion diseases of humans and animals: Their causes and molecular basis

      ANNUAL REVIEW OF NEUROSCIENCE
    12. Baldwin, MA
      Mass spectrometric analysis of prion proteins

      ADVANCES IN PROTEIN CHEMISTRY, VOL 57
    13. Lee, DC; Stenland, CJ; Miller, JLC; Cai, K; Ford, EK; Gilligan, KJ; Hartwell, RC; Terry, JC; Rubenstein, R; Fournel, M; Petteway, SR
      A direct relationship between the partitioning of the pathogenic prion protein and transmissible spongiform encephalopathy infectivity during the purification of plasma proteins

      TRANSFUSION
    14. Dell, A; Morris, HR
      Glycoprotein structure determination mass spectrometry

      SCIENCE
    15. Shaked, Y; Rosenmann, H; Hijazi, N; Halimi, M; Gabizon, R
      Copper binding to the PrP isoforms: a putative marker of their conformation and function

      JOURNAL OF VIROLOGY
    16. Xiong, LW; Raymond, LD; Hayes, SF; Raymond, GJ; Caughey, B
      Conformational change, aggregation and fibril formation induced by detergent treatments of cellular prion protein

      JOURNAL OF NEUROCHEMISTRY
    17. Cooley, WA; Clark, JK; Ryder, SJ; Davis, LA; Farrelly, SSJ; Stack, MJ
      Evaluation of a rapid western immunoblotting procedure for the diagnosis of bovine spongiform encephalopathy (BSE) in the UK

      JOURNAL OF COMPARATIVE PATHOLOGY
    18. Speransky, VV; Taylor, KL; Edskes, HK; Wickner, RB; Steven, AC
      Prion filament networks in [URE3] cells of Saccharomyces cerevisiae

      JOURNAL OF CELL BIOLOGY
    19. Kozin, SA; Bertho, G; Mazur, AK; Rabesona, H; Girault, JP; Haertle, T; Takahashi, M; Debey, P; Hoa, GHB
      Sheep prion protein synthetic peptide spanning helix 1 and beta-strand 2 (residues 142-166) shows beta-hairpin structure in solution

      JOURNAL OF BIOLOGICAL CHEMISTRY
    20. Shaked, GM; Shaked, Y; Kariv-Inbal, Z; Halimi, M; Avraham, I; Gabizon, R
      A protease-resistant prion protein isoform is present in urine of animals and humans affected with prion diseases

      JOURNAL OF BIOLOGICAL CHEMISTRY
    21. Kramer, ML; Kratzin, HD; Schmidt, B; Romer, A; Windl, O; Liemann, S; Hornemann, S; Kretzschmar, H
      Prion protein binds copper within the physiological concentration range

      JOURNAL OF BIOLOGICAL CHEMISTRY
    22. Piccardo, P; Liepnieks, JJ; William, A; Dlouhy, SR; Farlow, MR; Young, K; Nochlin, D; Bird, TD; Nixon, RR; Ball, MJ; DeCarli, C; Bugiani, O; Tagliavini, F; Benson, MD; Ghetti, B
      Prion proteins with different conformations accumulate in Geustmann-Straussler-Scheinker disease caused by A117V and F198S mutations

      AMERICAN JOURNAL OF PATHOLOGY
    23. Parchi, P; Capellari, S; Gambetti, P
      Intracerebral distribution of the abnormal isoform of the prion protein insporadic Creutzfeldt-Jakob disease and fatal insomnia

      MICROSCOPY RESEARCH AND TECHNIQUE
    24. Wang, DW; Yang, HY; Rao, ZH; Tian, B
      High-level expression and secondary structure analysis of the bovine mature prion protein

      CHINESE SCIENCE BULLETIN
    25. Jackson, GS; Clarke, AR
      Mammalian prion proteins

      CURRENT OPINION IN STRUCTURAL BIOLOGY
    26. Parchment, OG; Essex, JW
      Molecular dynamics of mouse and syrian hamster PrP: Implications for activity

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    27. Politopoulou, G; Seebach, JD; Schmugge, M; Schwarz, HP; Aguzzi, A
      Age-related expression of the cellular prion protein in human peripheral blood leukocytes

      HAEMATOLOGICA
    28. Chen, SG; Zou, W; Parchi, P; Gambetti, P
      PrPSc typing by N-terminal sequencing and mass spectrometry

      ARCHIVES OF VIROLOGY
    29. Vorberg, I; Pfaff, E; Groschup, MH
      The use of monoclonal antibody epitopes for tagging PrP in conversion experiments

      ARCHIVES OF VIROLOGY
    30. Nakaoke, R; Sakaguchi, S; Atarashi, R; Nishida, N; Arima, K; Shigematsu, K; Katamine, S
      Early appearance but lagged accumulation of detergent-insoluble prion protein in the brains of mice inoculated with a mouse-adapted Creutzfeldt-Jakobdisease agent

      CELLULAR AND MOLECULAR NEUROBIOLOGY
    31. Brown, DR; Hafiz, F; Glasssmith, LL; Wong, BS; Jones, IM; Clive, C; Haswell, SJ
      Consequences of manganese replacement of copper for prion protein functionand proteinase resistance

      EMBO JOURNAL
    32. Lee, DC; Stenland, CJ; Hartwell, RC; Ford, EK; Cai, K; Miller, JLC; Gilligan, KJ; Rubenstein, R; Fournel, M; Petteway, SR
      Monitoring plasma processing steps with a sensitive Western blot assay forthe detection of the prion protein

      JOURNAL OF VIROLOGICAL METHODS
    33. Bieschke, J; Giese, A; Schulz-Schaeffer, W; Zerr, I; Poser, S; Eigen, M; Kretzschmar, H
      Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets

      PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    34. Chapron, Y; Peyrin, JM; Crouzy, S; Jaegly, A; Dormont, D
      Theoretical analysis of the implication of PrP in neuronal death during transmissible subacute spongiform encephalopathies: Hypothesis of a PrP oligomeric channel

      JOURNAL OF THEORETICAL BIOLOGY
    35. Inouye, H; Bond, J; Baldwin, MA; Ball, HL; Prusiner, SB; Kirschner, DA
      Structural changes in a hydrophobic domain of the prion protein induced byhydration and by Ala -> Val and Pro -> Leu substitutions

      JOURNAL OF MOLECULAR BIOLOGY
    36. Meyer, RK; Lustig, A; Oesch, B; Fatzer, R; Zurbriggen, A; Vandevelde, M
      A monomer-dimer equilibrium of a cellular prion protein (PrPC) not observed with recombinant PrP

      JOURNAL OF BIOLOGICAL CHEMISTRY
    37. Qin, KF; Yang, DS; Yang, Y; Chishti, MA; Meng, LJ; Kretzschmar, HA; Yip, CM; Fraser, PE; Westaway, D
      Copper(II)-induced conformational changes and protease resistance in recombinant and cellular PrP - Effect of protein age and deamidation

      JOURNAL OF BIOLOGICAL CHEMISTRY
    38. Brown, DR; Iordanova, NK; Wong, BS; Venien-Bryan, C; Hafiz, F; Glasssmith, LL; Sy, MS; Gambetti, P; Jones, IM; Clive, C; Haswell, SJ
      Functional and structural differences between the prion protein from two alleles prnp(a) and prnp(b) of mouse

      EUROPEAN JOURNAL OF BIOCHEMISTRY
    39. Durig, J; Giese, A; Schulz-Schaeffer, W; Rosenthal, C; Schmucker, U; Bieschke, J; Duhrsen, U; Kretzschmar, HA
      Differential constitutive and activation-dependent expression of prion protein in human peripheral blood leucocytes

      BRITISH JOURNAL OF HAEMATOLOGY
    40. Lu, K; Wang, W; Xie, ZL; Wong, BS; Li, RL; Petersen, RB; Sy, MS; Chen, SG
      Expression and structural characterization of the recombinant human doppelprotein

      BIOCHEMISTRY
    41. Baskakov, IV; Aagaard, C; Mehlhorn, I; Wille, H; Groth, D; Baldwin, MA; Prusiner, SB; Cohen, FE
      Self-assembly of recombinant prion protein of 106 residues

      BIOCHEMISTRY
    42. Ma, JY; Lindquist, S
      De novo generation of a PrPSc-like conformation in living cells

      NATURE CELL BIOLOGY
    43. Page-McCaw, PS; Amonlirdviman, K; Sharp, PA
      PUF60: A novel U2AF65-related splicing activity

      RNA-A PUBLICATION OF THE RNA SOCIETY
    44. Takahashi, H; Takahashi, RH; Hasegawa, H; Horiuchi, M; Shinagawa, M; Yokoyama, T; Kimura, K; Haritani, M; Kurata, T; Nagashima, K
      Characterization of antibodies raised against bovine-PrP-peptides

      JOURNAL OF NEUROVIROLOGY
    45. Wickner, RB; Taylor, KL; Edskes, HK; Maddelein, ML; Moriyama, H; Roberts, BT
      Prions in Saccharomyces and Podospora spp.: Protein-based inheritance

      MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
    46. Ratovitski, T; Corson, LB; Strain, J; Wong, P; Cleveland, DW; Culotta, VC; Borchelt, DR
      Variation in the biochemical/biophysical properties of mutant superoxide dismutase 1 enzymes and the rate of disease progression in familial amyotrophic lateral sclerosis kindreds

      HUMAN MOLECULAR GENETICS
    47. Masel, J; Jansen, VAA
      The kinetics of proteinase K digestion of linear prion polymers

      PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
    48. Weihl, CC; Roos, RP
      Creutzfeldt-Jakob disease, new variant Creutzfeldt-Jakob disease, and bovine spongiform encephalopathy

      NEUROLOGIC CLINICS
    49. Parchi, P; Giese, A; Capellari, S; Brown, P; Schulz-Schaeffer, W; Windl, O; Zerr, I; Budka, H; Kopp, N; Piccardo, P; Poser, S; Rojiani, A; Streichemberger, N; Julien, J; Vital, C; Ghetti, B; Gambetti, P; Kretzschmar, H
      Classification of sporadic Creutzfeldt-Jakob disease based on molecular and phenotypic analysis of 300 subjects

      ANNALS OF NEUROLOGY
    50. Brown, DR
      Prion protein expression aids cellular uptake and veratridine-induced release of copper

      JOURNAL OF NEUROSCIENCE RESEARCH
    51. Maddelein, ML; Wickner, RB
      Two prion-inducing regions of Ure2p are nonoverlapping

      MOLECULAR AND CELLULAR BIOLOGY
    52. Salmona, M; Malesani, P; De Gioia, L; Gorla, S; Bruschi, M; Molinari, A; Della Vedova, F; Pedrotti, B; Marrari, MA; Awan, T; Bugiani, O; Forloni, G; Tagliavini, F
      Molecular determinants of the physicochemical properties of a critical prion protein region comprising residues 106-126

      BIOCHEMICAL JOURNAL
    53. Fernandez-Bellot, E; Guillemet, E; Baudin-Baillieu, A; Gaumer, S; Komar, AA; Cullin, C
      Characterization of the interaction domains of Ure2p, a prion-like proteinof yeast

      BIOCHEMICAL JOURNAL
    54. Jackson, GS; Hill, SF; Joseph, C; Hosszu, L; Power, A; Waltho, JP; Clarke, AR; Collinge, J
      Multiple folding pathways for heterologously expressed human prion protein

      BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
    55. Martins, VR
      A receptor for infectious and cellular prion protein

      BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
    56. Tan, LJ; Williams, MA; Khan, MK; Champion, HC; Nielsen, NH
      Risk of transmission of bovine spongiform encephalopathy to humans in the United States - Report of the Council on Scientific Affairs

      JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
    57. Korth, C; Streit, P; Oesch, B
      Monoclonal antibodies specific for the native, disease-associated isoform of the prion protein

      AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES
    58. Serpell, LC; Fraser, PE; Sunde, M
      X-ray fiber diffraction of amyloid fibrils

      AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES
    59. Baldwin, MA
      Stable isotope-labeled peptides in study of protein aggregation

      AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES
    60. Belay, ED
      Transmissible spongiform encephalopathies in humans

      ANNUAL REVIEW OF MICROBIOLOGY
    61. Edskes, HK; Gray, VT; Wickner, RB
      The [URE3] prion is an aggregated form of Ure2p that can be cured by overexpression of Ure2p fragments

      PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    62. Meyer, RK; Oesch, B; Fatzer, R; Zurbriggen, A; Vandevelde, N
      Detection of bovine spongiform encephalopathy-specific PrPSc by treatment with heat and guanidine thiocyanate

      JOURNAL OF VIROLOGY
    63. Sogal, A; Tofe, AJ
      Risk assessment of bovine spongiform encephalopathy transmission through bone graft material derived from bovine bone used for dental applications

      JOURNAL OF PERIODONTOLOGY
    64. Cohen, FE
      Protein misfolding and prion diseases

      JOURNAL OF MOLECULAR BIOLOGY
    65. Shaked, Y; Rosenmann, H; Talmor, G; Gabizon, R
      A C-terminal-truncated PrP isoform is present in mature sperm

      JOURNAL OF BIOLOGICAL CHEMISTRY
    66. Weissmann, C
      Molecular genetics of transmissible spongiform encephalopathies

      JOURNAL OF BIOLOGICAL CHEMISTRY
    67. McKie, AT; Zammit, PS; Naftalin, RJ
      Comparison of cattle and sheep colonic permeabilities to horseradish peroxidase and hamster scrapie prion protein in vitro

      GUT
    68. Marcotte, EM; Eisenberg, D
      Chicken prion tandem repeats form a stable, protease-resistant domain

      BIOCHEMISTRY
    69. Liu, H; Farr-Jones, S; Ulyanov, NB; Llinas, M; Marqusee, S; Groth, D; Cohen, FE; Prusiner, SB; James, TL
      Solution structure of Syrian hamster prion protein rPrP(90-231)

      BIOCHEMISTRY
    70. Kajiwara, H
      Affinity capillary electrophoresis of proteins and peptides

      ANALYTICA CHIMICA ACTA
    71. GALE P; YOUNG C; STANFIELD G; OAKES D
      DEVELOPMENT OF A RISK ASSESSMENT FOR BSE IN THE AQUATIC ENVIRONMENT

      Journal of applied microbiology
    72. INOUYE H; DOMINGUES FS; DAMAS AM; SARAIVA MJ; LUNDGREN E; SANDGREN O; KIRSCHNER DA
      ANALYSIS OF X-RAY-DIFFRACTION PATTERNS FROM AMYLOID OF BIOPSIED VITREOUS-HUMOR AND KIDNEY OF TRANSTHYRETIN (TTR) MET30 FAMILIAL AMYLOIDOTICPOLYNEUROPATHY (FAP) PATIENTS - AXIALLY ARRAYED TTR MONOMERS CONSTITUTE THE PROTOFILAMENT

      Amyloid (Carnforth)
    73. Kirschner, DA; Elliott-Bryant, R; Szumowski, KE; Gonnerman, WA; Kindy, MS; Sipe, JD; Cathcart, ES
      In vitro amyloid fibril formation by synthetic peptides corresponding to the amino terminus of apoSAA isoforms from amyloid-susceptible and amyloid-resistant mice

      JOURNAL OF STRUCTURAL BIOLOGY
    74. JAEGLY A; MOUTHON F; PEYRIN JM; CAMUGLI B; DESLYS JP; DORMONT D
      SEARCH FOR A NUCLEAR-LOCALIZATION SIGNAL IN THE PRION PROTEIN

      Molecular and cellular neurosciences (Print)
    75. BROWN DR; SCHMIDT B; KRETZSCHMAR HA
      A PRION PROTEIN-FRAGMENT PRIMES TYPE-1 ASTROCYTES TO PROLIFERATION SIGNALS FROM MICROGLIA

      Neurobiology of disease
    76. BROWN DR; BESINGER A; HERMS JW; KRETZSCHMAR HA
      MICROGLIAL EXPRESSION OF THE PRION PROTEIN

      NeuroReport
    77. HERRMANN LM; CAUGHEY B
      THE IMPORTANCE OF THE DISULFIDE BOND IN PRION PROTEIN CONVERSION

      NeuroReport
    78. FISHER E; TELLING G; COLLINGE J
      PRIONS AND THE PRION DISORDERS

      Mammalian genome
    79. BROWN DR; PITSCHKE M; RIESNER D; KRETZSCHMAR HA
      CELLULAR EFFECTS OF A NEUROTOXIC PRION PROTEIN PEPTIDE ARE RELATED TOITS BETA-SHEET CONTENT

      Neuroscience research communications
    80. BROWN DR
      PRION PROTEIN-OVEREXPRESSING CELLS SHOW ALTERED RESPONSE TO A NEUROTOXIC PRION PROTEIN PEPTIDE

      Journal of neuroscience research
    81. BROWN DR; SCHMIDT B; KRETZSCHMAR HA
      PRION PROTEIN-FRAGMENT INTERACTS WITH PRP-DEFICIENT CELLS

      Journal of neuroscience research
    82. ADAMS DH
      A DUAL PATHWAY FOR THE TRANSMISSION OF SPONGIFORM ENCEPHALOPATHY DISEASE

      Medical hypotheses
    83. TASHIRO H; DOHURA K; IWAKI T
      DIFFERENTIAL EXPRESSION OF TRANSFORMING-GROWTH-FACTOR-BETA ISOFORMS IN HUMAN PRION DISEASES

      Neuropathology and applied neurobiology
    84. BALDWIN MA; JAMES TL; COHEN FE; PRUSINER SB
      THE 3-DIMENSIONAL STRUCTURE OF PRION PROTEIN - IMPLICATIONS FOR PRIONDISEASE

      Biochemical Society transactions
    85. Katamine, S; Nishida, N; Sugimoto, T; Noda, T; Sakaguchi, S; Shigematsu, G; Kataoka, Y; Hasegawa, S; Moriuchi, R; Miyamoto, T
      Impaired motor coordination in mice lacking prion protein

      CELLULAR AND MOLECULAR NEUROBIOLOGY
    86. PEREZ M; WANDOSELL F; COLACO C; AVILA J
      SULFATED GLYCOSAMINOGLYCANS PREVENT THE NEUROTOXICITY OF A HUMAN PRION PROTEIN-FRAGMENT

      Biochemical journal
    87. BROWN DR; BESINGER A
      PRION PROTEIN EXPRESSION AND SUPEROXIDE-DISMUTASE ACTIVITY

      Biochemical journal
    88. MAYOR S; SABHARANJAK S; MAXFIELD FR
      CHOLESTEROL-DEPENDENT RETENTION OF GPI-ANCHORED PROTEINS IN ENDOSOMES

      EMBO journal (Print)
    89. WEISSMANN C; FISCHER M; RAEBER A; BUELER H; SAILER A; SHMERLING D; RULICKE T; BRANDNER S; AGUZZI A
      THE USE OF TRANSGENIC MICE IN THE INVESTIGATION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES

      Revue scientifique et technique - Office international des epizooties
    90. BROWN DR; SCHMIDT B; GROSCHUP MH; KRETZSCHMAR HA
      PRION PROTEIN EXPRESSION IN MUSCLE-CELLS AND TOXICITY OF A PRION PROTEIN-FRAGMENT

      European journal of cell biology
    91. COHEN FE; PRUSINER SB
      PATHOLOGICAL CONFORMATIONS OF PRION PROTEINS

      Annual review of biochemistry
    92. ZAVALISHIN IA; ROIKHEL VM; ZHUCHENKO TD; SHITIKOVA IE
      HUMAN PRIONIC DISEASES

      Zurnal nevropatologii i psihiatrii im. S.S. Korsakova
    93. SUNDE M; BLAKE CCF
      FROM THE GLOBULAR TO THE FIBROUS STATE - PROTEIN-STRUCTURE AND STRUCTURAL CONVERSION IN AMYLOID FORMATION

      Quarterly Reviews of Biophysics
    94. GARCIA AD; ALBERT EM; SANMARTI LS
      PRIONS AND TRANSMISSIBLE NEURODEGENERATIV E DISEASES

      Medicina Clinica
    95. DEMAIMAY R; HARPER J; GORDON H; WEAVER D; CHESEBRO B; CAUGHEY B
      STRUCTURAL ASPECTS OF CONGO RED AS AN INHIBITOR OF PROTEASE-RESISTANTPRION PROTEIN-FORMATION

      Journal of neurochemistry
    96. BROWN DR; SCHMIDT B; KRETZSCHMAR HA
      EFFECTS OF COPPER ON SURVIVAL OF PRION PROTEIN KNOCKOUT NEURONS AND GLIA

      Journal of neurochemistry
    97. KRAKAUER DC; ZANOTTO PMD; PAGEL M
      PRIONS PROGRESS - PATTERNS AND RATES OF MOLECULAR EVOLUTION IN RELATION TO SPONGIFORM DISEASE

      Journal of molecular evolution
    98. VANDERVALK P
      PRION DISEASES - WHAT WILL BE NEXT

      Journal of Clinical Pathology
    99. PRIOLA SA; CHESEBRO B
      ABNORMAL PROPERTIES OF PRION PROTEIN WITH INSERTIONAL MUTATIONS IN DIFFERENT CELL-TYPES

      The Journal of biological chemistry
    100. VOLKEL D; BLANKENFELDT W; SCHOMBURG D
      LARGE-SCALE PRODUCTION, PURIFICATION AND REFOLDING OF THE FULL-LENGTHCELLULAR PRION PROTEIN FROM SYRIAN GOLDEN-HAMSTER IN ESCHERICHIA-COLIUSING THE GLUTATHIONE-S-TRANSFERASE FUSION SYSTEM

      European journal of biochemistry


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Documento generato il 10/08/20 alle ore 08:31:31