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La ricerca find articoli where soggetti phrase all words 'PERFUSION CULTURE' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 93 riferimenti
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    1. Kremer, M; Lang, E; Berger, A
      Organotypical engineering of differentiated composite-skin equivalents of human keratinocytes in a collagen-GAG matrix (INTEGRA Artificial Skin) in aperfusion culture system

      LANGENBECKS ARCHIVES OF SURGERY
    2. Johnson, DH; Johnson, M
      How does nonpenetrating glaucoma surgery work? Aqueous outflow resistance and glaucoma surgery

      JOURNAL OF GLAUCOMA
    3. Minuth, WW; Strehl, R; Schumacher, K; De Vries, U
      Long term culture of epithelia in a continuous fluid gradient for biomaterial testing and tissue engineering

      JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
    4. Schumacher, K; Klotz-Vangerow, S; Tauc, M; Minuth, WW
      Embryonic renal collecting duct cell differentiation is influenced in a concentration-dependent manner by the electrolyte environment

      AMERICAN JOURNAL OF NEPHROLOGY
    5. Liu, J; Jauregui, HO; Faris, RA; Santangini, HA; Trenkler, DM; Silva, PG; Garcia, KM
      Growth and metabolic activity of immortalized porcine hepatocytes in extracorporeal hollow-fiber liver assist devices

      ARTIFICIAL ORGANS
    6. Schultz, O; Sittinger, M; Haeupl, T; Burmester, GR
      Emerging strategies of bone and joint repair

      ARTHRITIS RESEARCH
    7. Rudert, M; Hirschmann, F; Schulze, M; Wirth, CJ
      Bioartificial cartilage

      CELLS TISSUES ORGANS
    8. Yamaji, H; Tagai, SI; Sakai, K; Izumoto, E; Fukuda, H
      Production of recombinant protein by baculovirus-infected insect cells in immobilized culture using porous biomass support particles

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    9. Morimoto, T; Tashiro, F; Nagashima, H; Nishizawa, K; Nagata, F; Yokogawa, Y; Suzuki, T
      Production of poly-beta-hydroxybutyric acid by microorganisms accumulated from river water using a two-stage perfusion culture system

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    10. Pang, IH; McCartney, MD; Steely, HT; Clark, AF
      Human ocular perfusion organ culture: A versatile ex vivo model for glaucoma research

      JOURNAL OF GLAUCOMA
    11. Merten, OW
      Constructive improvement of the ultrasonic separation device ADI 1015

      CYTOTECHNOLOGY
    12. Heidemann, R; Zhang, C; Qi, HS; Larrick Rule, J; Rozales, C; Park, S; Chuppa, S; Ray, M; Michaels, J; Konstantinov, K; Naveh, D
      The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammalian cells

      CYTOTECHNOLOGY
    13. Minuth, WW; Schumacher, K; Strehl, R; Kloth, S
      Physiological and cell biological aspects of perfusion culture technique employed to generate differentiated tissues for long term biomaterial testing and tissue engineering

      JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
    14. Kloth, S; Kobuch, K; Domokos, J; Wanke, C; Monzer, J
      Polar application of test substances in organotypic environment and under continuous medium flow: A new tissue-based test concept for a broad range of applications in pharmacotoxicology

      TOXICOLOGY IN VITRO
    15. Maitz, M; Trampler, F; Groschl, M; Machado, AD; Machado, MLD
      Use of an ultrasound cell retention system for the size fractionation of somatic embryos of woody species

      PLANT CELL REPORTS
    16. Wen, ZY; Teng, XW; Chen, F
      A novel perfusion system for animal cell cultures by two step sequential sedimentation

      JOURNAL OF BIOTECHNOLOGY
    17. Temenoff, JS; Mikos, AG
      Review: tissue engineering for regeneration of articular cartilage

      BIOMATERIALS
    18. Niederauer, GG; Slivka, MA; Leatherbury, NC; Korvick, DL; Harroff, HH; Ehler, WC; Dunn, CJ; Kieswetter, K
      Evaluation of multiphase implants for repair of focal osteochondral defects in goats

      BIOMATERIALS
    19. Cohen, I; Robinson, D; Cohen, N; Nevo, Z
      Storing live embryonic and adult human cartilage grafts for transplantation using a joint simulating device

      BIOMATERIALS
    20. Chen, CY; Halkos, ME; Surowiec, SM; Conklin, BS; Lin, PH; Lumsden, AB
      Effects of homocysteine on smooth muscle cell proliferation in both cell culture and artery perfusion culture models

      JOURNAL OF SURGICAL RESEARCH
    21. Surowiec, SM; Conklin, BS; Li, JS; Lin, PH; Weiss, VJ; Lumsden, AB; Chen, CY
      A new perfusion culture system used to study human vein

      JOURNAL OF SURGICAL RESEARCH
    22. Perka, C; Sittinger, M; Schultz, O; Spitzer, RS; Schlenzka, D; Burmester, GR
      Tissue engineered cartilage repair using cryopreserved and noncryopreserved chondrocytes

      CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
    23. Chico, E; Jager, V
      Perfusion culture of baculovirus-infected BTI-Tn-5B1-4 insect cells: A method to restore cell-specific beta-trace glycoprotein productivity at high cell density

      BIOTECHNOLOGY AND BIOENGINEERING
    24. Nakashimada, Y; Nakae, K; Nishio, N
      Inhibitory effect of acetic acid on growth of hyperthermophilic archaeon Pyrococcus furiosus

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    25. Schumacher, K; Strehl, R; Kloth, C; Tauc, M; Minuth, WW
      The influence of culture media on embryonic renal collecting duct cell differentiation

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
    26. Minuth, WW; Steiner, P; Strehl, R; Schumacher, K; de Vries, U; Kloth, S
      Modulation of cell differentiation in perfusion culture

      EXPERIMENTAL NEPHROLOGY
    27. Furukawa, K; Ohsuye, K
      Enhancement of productivity of recombinant alpha-amidating enzyme by low temperature culture

      CYTOTECHNOLOGY
    28. Leelavatcharamas, V; Emery, AN; Al-Rubeai, M
      Use of cell cycle analysis to characterise growth and interferon-gamma production in perfusion culture of CHO cells

      CYTOTECHNOLOGY
    29. Reischl, J; Prelle, K; Schol, H; Neumuller, C; Einspanier, R; Sinowatz, F; Wolf, E
      Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro

      CELL AND TISSUE RESEARCH
    30. Saadeh, PB; Brent, B; Mehrara, BJ; Steinbrech, DS; Ting, V; Gittes, GK; Longaker, MT
      Chondrocyte extraction, proliferation, and characterization for construct development

      ANNALS OF PLASTIC SURGERY
    31. Kim, JH; Park, JH; Kang, WK; Yoon, SK
      Perfusion culture using microcarrier for the production of Varicella-Zoster virus in human embryonic lung cells

      BIOTECHNOLOGY LETTERS
    32. Rudert, M; Hirschmann, F; Wirth, CJ
      Growth of chondrocytes on different biomaterials

      ORTHOPADE
    33. Takamatsu, H; Kawajiri, H; Takahashi, Y; Ali, AM; Yoshimoto, T
      Continuous antibody production by phytohemagglutinin-L-aggregated hybridoma cells

      JOURNAL OF IMMUNOLOGICAL METHODS
    34. Arnoczky, SP
      Building a meniscus - Biologic considerations

      CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
    35. NAUMANN A; ROTTER N; BUJIA J; AIGNER J
      TISSUE ENGINEERING OF AUTOLOGOUS CARTILAGE TRANSPLANTS FOR RHINOLOGY

      American journal of rhinology
    36. HEATH CA
      BIOCHEMICAL-ENGINEERING FROM MACROSCALE TO MICROSCALE PERSPECTIVES - EDITORIAL OVERVIEW

      Current opinion in biotechnology
    37. OHASHI R; MOCHIZUKI E; KAMOSHITA Y; SUZUKI T
      HIGH-LEVEL EXPRESSION OF THE METHANOL-INDUCIBLE BETA-GALACTOSIDASE GENE BY PERFUSION CULTURE OF RECOMBINANT PICHIA-PASTORIS USING A SHAKEN CERAMIC MEMBRANE FLASK

      Journal of fermentation and bioengineering
    38. KAMOSHITA Y; OHASHI R; SUZUKI T
      IMPROVEMENT OF FILTRATION PERFORMANCE OF STIRRED CERAMIC MEMBRANE REACTOR AND ITS APPLICATION TO RAPID FERMENTATION OF LACTIC-ACID BY DENSECELL-CULTURE OF LACTOCOCCUS-LACTIS

      Journal of fermentation and bioengineering
    39. DUTTON RL; SCHARER JM; MOOYOUNG M
      DESCRIPTIVE PARAMETER EVALUATION IN MAMMALIAN-CELL CULTURE

      Cytotechnology
    40. MINUTH WW; SITTINGER M; KLOTH S
      TISSUE ENGINEERING - GENERATION OF DIFFERENTIATED ARTIFICIAL TISSUES FOR BIOMEDICAL APPLICATIONS

      Cell and tissue research
    41. BIERAU H; PERANI A; ALRUBEAI M; EMERY AN
      A COMPARISON OF INTENSIVE CELL-CULTURE BIOREACTORS OPERATING WITH HYBRIDOMAS MODIFIED FOR INHIBITED APOPTOTIC RESPONSE

      Journal of biotechnology
    42. KOLLER MR; PALSSON MA; MANCHEL I; MAHER RJ; PALSSON BO
      TISSUE-CULTURE SURFACE CHARACTERISTICS INFLUENCE THE EXPANSION OF HUMAN BONE-MARROW CELLS

      Biomaterials
    43. SUGIURA T; KAKUZAKI M
      DYNAMICS OF RECOMBINANT PROTEIN-PRODUCTION BY MAMMALIAN-CELLS IN IMMOBILIZED PERFUSION CULTURE

      Enzyme and microbial technology
    44. ROTTER N; AIGNER J; NAUMANN A; PLANCK H; HAMMER C; BURMESTER G; SITTINGER M
      CARTILAGE RECONSTRUCTION IN HEAD AND NECK-SURGERY - COMPARISON OF RESORBABLE POLYMER SCAFFOLDS FOR TISSUE ENGINEERING OF HUMAN SEPTAL CARTILAGE

      Journal of biomedical materials research
    45. AIGNER J; TEGELER J; HUTZLER P; CAMPOCCIA D; PAVESIO A; HAMMER C; KASTENBAUER E; NAUMANN A
      CARTILAGE TISSUE ENGINEERING WITH NOVEL NONWOVEN STRUCTURED BIOMATERIAL BASED ON HYALURONIC-ACID BENZYL ESTER

      Journal of biomedical materials research
    46. GOLDMAN MH; JAMES DC; RENDALL M; ISON AP; HOARE M; BULLL AT
      MONITORING RECOMBINANT HUMAN INTERFERON-GAMMA N-GLYCOSYLATION DURING PERFUSED FLUIDIZED-BED AND STIRRED-TANK BATCH CULTURE OF CHO CELLS

      Biotechnology and bioengineering
    47. CAIN SJ; CHAU PC
      TRANSITION-PROBABILITY CELL-CYCLE MODEL WITH PRODUCT FORMATION

      Biotechnology and bioengineering
    48. AIGNER J; HUTZLER P; BUJIA J; KASTENBAUER E
      DISTRIBUTION AND VIABILITY OF CULTURED HUMAN CHONDROCYTES IN A 3-DIMENSIONAL MATRIX AS ASSESSED BY CONFOCAL LASER SCAN MICROSCOPY

      In vitro cellular & developmental biology. Animal
    49. MINUTH WW; STEINER P; STREHL R; KLOTH S; TAUC M
      ELECTROLYTE ENVIRONMENT MODULATES DIFFERENTIATION IN EMBRYONIC RENAL COLLECTING DUCT EPITHELIA

      Experimental nephrology
    50. AIGNER J; WILMES E; NAUMANN A; BUJIA J
      CONFOCAL LASER-SCANNING MICROSCOPY ENABLE S REALISTIC RECONSTRUCTION OF CELL SYSTEMS AS ILLUSTRATED WITH 3-DIMENSIONAL CULTURED CHONDROCYTES

      Laryngo-, Rhino-, Otologie
    51. MINUTH WW; AIGNER J; KLOTH S; STEINER P; TAUC M; JENNINGS ML
      CULTURE OF EMBRYONIC RENAL COLLECTING DUCT EPITHELIA IN A GRADIENT CONTAINER

      Pediatric nephrology
    52. YAMAJI H; FUKUDA H
      CONTINUOUS IGG PRODUCTION BY MYELOMA CELLS IMMOBILIZED WITHIN POROUS SUPPORT PARTICLES

      Journal of fermentation and bioengineering
    53. MERTEN OW; WU R; COUVE E; CRAINIC R
      EVALUATION OF THE SERUM-FREE MEDIUM MDSS2 FOR THE PRODUCTION OF POLIOVIRUS ON VERO CELLS IN BIOREACTORS

      Cytotechnology
    54. FONG WF; ZHANG YX; YUNG PP
      OPTIMIZATION OF MONOCLONAL-ANTIBODY PRODUCTION - COMBINED EFFECTS OF POTASSIUM ACETATE AND PERFUSION IN A STIRRED-TANK BIOREACTOR

      Cytotechnology
    55. SITTINGER M; SCHULTZ O; KEYSZER G; MINUTH WW; BURMESTER GR
      ARTIFICIAL TISSUES IN PERFUSION CULTURE

      International journal of artificial organs
    56. WIRTZ MK; BRADLEY JMB; XU H; DOMREIS J; NOBIS CA; TRUESDALE AT; SAMPLES JR; VANBUSKIRK EM; ACOTT TS
      PROTEOGLYCAN EXPRESSION BY HUMAN TRABECULAR MESHWORKS

      Current eye research
    57. COAKLEY WT
      ULTRASONIC SEPARATIONS IN ANALYTICAL BIOTECHNOLOGY

      Trends in biotechnology
    58. STREHL R; KLOTH S; AIGNER J; STEINER P; MINUTH WW
      P(CD)AMP1, A NEW ANTIGEN AT THE INTERFACE OF THE EMBRYONIC COLLECTINGDUCT EPITHELIUM AND THE NEPHROGENIC MESENCHYME

      Kidney international
    59. CHUPPA S; TSAI YS; YOON S; SHACKLEFORD S; ROZALES C; BHAT R; TSAY G; MATANGUIHAN C; KONSTANTINOV K; NAVEH D
      FERMENTER TEMPERATURE AS A POOL FOR CONTROL OF HIGH-DENSITY PERFUSIONCULTURES OF MAMMALIAN-CELLS

      Biotechnology and bioengineering
    60. DOCOSLIS A; KALOGERAKIS N; BEHIE LA; KALER KVIS
      A NOVEL DIELECTROPHORESIS-BASED DEVICE FOR THE SELECTIVE RETENTION OFVIABLE CELLS IN CELL-CULTURE MEDIA

      Biotechnology and bioengineering
    61. NAKANO K; KATAOKA H; MATSUMURA M
      HIGH-DENSITY CULTURE OF PROPIONIBACTERIUM-FREUDENREICHII COUPLED WITHPROPIONIC-ACID REMOVAL SYSTEM WITH ACTIVATED-CHARCOAL

      Journal of fermentation and bioengineering
    62. JAGER V
      PERFUSION BIOREACTORS FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN INSECT CELLS

      Cytotechnology
    63. TAKAHASHI K; UEDA H; SUZUKI E
      TEMPERATURE CHARACTERISTICS OF MAMMALIAN- CELL PERFUSION CULTURE

      Kagaku kogaku ronbunshu
    64. ROGERS CE; BRADLEY MS; PALSSON BO; KOLLER MR
      FLOW CYTOMETRIC ANALYSIS OF HUMAN BONE-MARROW PERFUSION CULTURES - ERYTHROID DEVELOPMENT AND RELATIONSHIP WITH BURST-FORMING UNITS-ERYTHROID

      Experimental hematology
    65. PORTNER R; SCHAFER T
      MODELING HYBRIDOMA CELL-GROWTH AND METABOLISM A COMPARISON OF SELECTED MODELS AND DATA

      Journal of biotechnology
    66. TAGUCHI K; MATSUSHITA M; TAKAHASHI M; UCHINO J
      DEVELOPMENT OF A BIOARTIFICIAL LIVER WITH SANDWICHED-CULTURED HEPATOCYTES BETWEEN 2 COLLAGEN GEL LAYERS

      Artificial organs
    67. PARSHLEY DE; BRADLEY JMB; FISK A; HADAEGH A; SAMPLES JR; VANBUSKIRK EM; ACOTT TS
      LASER TRABECULOPLASTY INDUCES STROMELYSIN EXPRESSION BY TRABECULAR JUXTACANALICULAR CELLS

      Investigative ophthalmology & visual science
    68. SITTINGER M; BUJIA J; ROTTER N; REITZEL D; MINUTH WW; BURMESTER GR
      TISSUE ENGINEERING AND AUTOLOGOUS TRANSPLANT FORMATION - PRACTICAL APPROACHES WITH RESORBABLE BIOMATERIALS AND NEW CELL-CULTURE TECHNIQUES

      Biomaterials
    69. KONSTANTINOV KB
      MONITORING AND CONTROL OF THE PHYSIOLOGICAL-STATE OF CELL-CULTURES

      Biotechnology and bioengineering
    70. MATSUMURA M; NAYVE FRP
      EFFECTS OF AMMONIUM ION REMOVAL ON GROWTH AND MAB PRODUCTION OF HYBRIDOMA CELLS

      Cytotechnology
    71. PALSSON BO; OH DJ; KOLLER MR
      REPLATING OF BIOREACTOR EXPANDED HUMAN BONE-MARROW RESULTS IN EXTENDED GROWTH OF PRIMITIVE AND MATURE CELLS

      Cytotechnology
    72. KLOTH S; EBENBECK C; KUBITZA M; SCHMIDBAUER A; ROCKL W; MINUTH WW
      STIMULATION OF RENAL MICROVASCULAR DEVELOPMENT UNDER ORGANOTYPIC CULTURE CONDITIONS

      The FASEB journal
    73. KOLLER MR; BRADLEY MS; PALSSON BO
      GROWTH-FACTOR CONSUMPTION AND PRODUCTION IN PERFUSION CULTURES OF HUMAN BONE-MARROW CORRELATE WITH SPECIFIC CELL PRODUCTION

      Experimental hematology
    74. TAKESHITA K; ISHIBASHI H; SUZUKI M; YAMAMOTO T; AKAIKE T; KODAMA M
      HIGH CELL-DENSITY CULTURE SYSTEM OF HEPATOCYTES ENTRAPPED IN A 3-DIMENSIONAL HOLLOW-FIBER MODULE WITH COLLAGEN GEL

      Artificial organs
    75. CLARK AF; WILSON K; DEKATER AW; ALLINGHAM RR; MCCARTNEY MD
      DEXAMETHASONE-INDUCED OCULAR HYPERTENSION IN PERFUSION-CULTURED HUMANEYES

      Investigative ophthalmology & visual science
    76. KOHARA Y; UEDA H; SUZUKI E
      ENHANCED SETTLING OF MAMMALIAN-CELLS IN TANKS WITH INCLINED PLATES SIMULATION BY FLUID MECHANICAL MODEL AND EXPERIMENT

      Journal of Chemical Engineering of Japan
    77. OMASA T; KOBAYASHI M; NISHIKAWA T; SHIOYA S; SUGA K; UEMURA S; KITANI Y; IMAMURA Y
      ENHANCEMENT OF ANTIBODY-PRODUCTION BY GROWTH-FACTOR ADDITION IN PERFUSION AND HOLLOW-FIBER CULTURE SYSTEMS

      Biotechnology and bioengineering
    78. PELLETIER F; FONTEIX C; DASILVA AL; MARC A; ENGASSER JM
      SOFTWARE SENSORS FOR THE MONITORING OF PERFUSION CULTURES - EVALUATION OF THE HYBRIDOMA DENSITY AND THE MEDIUM COMPOSITION FROM GLUCOSE-CONCENTRATION MEASUREMENTS

      Cytotechnology
    79. LOVRECZ G; GRAY P
      USE OF ONLINE GAS-ANALYSIS TO MONITOR RECOMBINANT MAMMALIAN-CELL CULTURES

      Cytotechnology
    80. KAWAHARA H; MITSUDA S; KUMAZAWA E; TAKESHITA Y
      HIGH-DENSITY CULTURE OF FM-3A CELLS USING A BIOREACTOR WITH AN EXTERNAL TANGENTIAL-FLOW FILTRATION DEVICE

      Cytotechnology
    81. MULCHANDANI A; VOLESKY B
      PRODUCTION OF ACETONE-BUTANOL-ETHANOL BY CLOSTRIDIUM-ACETOBUTYLICUM USING A SPIN FILTER PERFUSION BIOREACTOR

      Journal of biotechnology
    82. DEUTSCHMANN SM; JAGER V
      OPTIMIZATION OF THE GROWTH-CONDITIONS OF SF21 INSECT CELLS FOR HIGH-DENSITY PERFUSION CULTURE IN STIRRED-TANK BIOREACTORS

      Enzyme and microbial technology
    83. OH DJ; CHOI SK; CHANG HN
      HIGH-DENSITY CONTINUOUS CULTURES OF HYBRIDOMA CELLS IN A DEPTH FILTERPERFUSION SYSTEM

      Biotechnology and bioengineering
    84. OH DJ; KOLLER MR; PALSSON BO
      FREQUENT HARVESTING FROM PERFUSED BANE MARROW CULTURES RESULTS IN INCREASED OVERALL CELL AND PROGENITOR EXPANSION

      Biotechnology and bioengineering
    85. PARK SH; RYU DDY
      CELL-CYCLE KINETICS AND MONOCLONAL-ANTIBODY PRODUCTIVITY OF HYBRIDOMACELLS DURING PERFUSION CULTURE

      Biotechnology and bioengineering
    86. ZHOU WC; HU WS
      ONLINE CHARACTERIZATION OF A HYBRIDOMA CELL-CULTURE PROCESS

      Biotechnology and bioengineering
    87. TOKASHIKI M; TAKAMATSU H
      PERFUSION CULTURE APPARATUS FOR SUSPENDED MAMMALIAN-CELLS

      Cytotechnology
    88. THOMMES J; GATGENS J; BISELLI M; RUNSTADLER PW; WANDREY C
      THE INFLUENCE OF DISSOLVED-OXYGEN TENSION ON THE METABOLIC-ACTIVITY OF AN IMMOBILIZED HYBRIDOMA POPULATION

      Cytotechnology
    89. HANSEN HA; DAMGAARD B; EMBORG C
      ENHANCED ANTIBODY-PRODUCTION ASSOCIATED WITH ALTERED AMINO-ACID-METABOLISM IN A HYBRIDOMA HIGH-DENSITY PERFUSION CULTURE ESTABLISHED BY GRAVITY SEPARATION

      Cytotechnology
    90. SU WW; LEI F; SU LY
      PERFUSION STRATEGY FOR ROSMARINIC ACID PRODUCTION BY ANCHUSA-OFFICINALIS

      Biotechnology and bioengineering
    91. LIN AA; KIMURA R; MILLER WM
      PRODUCTION OF TPA IN RECOMBINANT CHO CELLS UNDER OXYGEN-LIMITED CONDITIONS

      Biotechnology and bioengineering
    92. HILLER GW; CLARK DS; BLANCH HW
      CELL RETENTION-CHEMOSTAT STUDIES OF HYBRIDOMA CELLS - ANALYSIS OF HYBRIDOMA GROWTH AND METABOLISM IN CONTINUOUS SUSPENSION-CULTURE ON SERUM-FREE MEDIUM

      Biotechnology and bioengineering
    93. JO EC; PARK HJ; KIM DI; MOON HM
      REPEATED FED-BATCH CULTURE OF HYBRIDOMA CELLS IN NUTRIENT-FORTIFIED HIGH-DENSITY MEDIUM

      Biotechnology and bioengineering


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Documento generato il 12/08/20 alle ore 18:12:23