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La ricerca find articoli where soggetti phrase all words 'LIGAND INTERACTION' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 281 riferimenti
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    1. de Herve, MGD; Delfraissy, JF; Taoufik, Y
      Following direct CD40 activation, human primary microglial cells produce IL-12 p40 but not bioactive IL-12 p70

      CYTOKINE
    2. Sin, JI; Kim, JJ; Zhang, DH; Weiner, DB
      Modulation of cellular responses by plasmid CD40L: CD40L plasmid vectors enhance antigen-specific helper T cell type 1 CD4(+) T cell-mediated protective immunity against herpes simplex virus type 2 in vivo

      HUMAN GENE THERAPY
    3. Demchenko, AP
      Recognition between flexible protein molecules: induced and assisted folding

      JOURNAL OF MOLECULAR RECOGNITION
    4. Li, DQ; Ljungh, A
      Binding of human clusterin by Staphylococcus epidermidis

      FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
    5. Mills, JEJ; de Esch, IJP; Perkins, TDJ; Dean, PM
      SLATE: A method for the superposition of flexible ligands

      JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN
    6. Ueta, H; Maeda, Y; Kawai, Y
      Features of distamycin preferential binding sites on natural DNA predictedusing differential scanning calorimetry

      BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
    7. Kaasgaard, T; Mouritsen, OG; Jorgensen, K
      Receptor mediated binding of avidin to polymer covered liposomes

      JOURNAL OF LIPOSOME RESEARCH
    8. Chen, YZ; Zhi, DG
      Ligand-protein inverse docking and its potential use in the computer search of protein targets of a small molecule

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    9. Rossi, C; Bonechi, C; Martini, S; Ricci, M; Corbini, G; Corti, P; Donati, A
      Ligand-macromolecule complexes: affinity index determination by selective nuclear relaxation analysis

      MAGNETIC RESONANCE IN CHEMISTRY
    10. Nandagopal, K; Popp, DM; Niyogi, SK
      Utilization of a receptor reserve for effective amplification of mitogenicsignaling by an epidermal growth factor mutant deficient in receptor activation

      JOURNAL OF CELLULAR BIOCHEMISTRY
    11. Kaasgaard, T; Mouritsen, OG; Jorgensen, K
      Screening effect of PEG on avidin binding to liposome surface receptors

      INTERNATIONAL JOURNAL OF PHARMACEUTICS
    12. Bullens, DMA; Kasran, A; Thielemans, K; Bakkus, M; Ceuppens, JL
      CD40L-Induced IL-12 production is further enhanced by the Th2 cytokines IL-4 and IL-13

      SCANDINAVIAN JOURNAL OF IMMUNOLOGY
    13. Bank, I; Miranda, AF; Chess, L
      Mechanisms of cell-mediated myocytotoxicity in the peripheral blood of patients with inflammatory myopathies

      JOURNAL OF CLINICAL IMMUNOLOGY
    14. Lu, XJ; Sun, YQ; Shang, DZ; Wattam, B; Egglezou, S; Hughes, T; Hyde, E; Scully, M; Kakkar, V
      Evaluation of the role of proline residues flanking the RGD motif of dendroaspin, an inhibitor of platelet aggregation and cell adhesion

      BIOCHEMICAL JOURNAL
    15. Bhushan, A; Covey, LR
      CD40 : CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes

      IMMUNOLOGIC RESEARCH
    16. Town, T; Tan, J; Mullan, M
      CD40 signaling and Alzheimer's disease pathogenesis

      NEUROCHEMISTRY INTERNATIONAL
    17. Lacaze-Dufaure, C; Mineva, T; Russo, N
      On the interaction of Mo and Mo-2 with NH3, C2H4, and C3H6

      JOURNAL OF COMPUTATIONAL CHEMISTRY
    18. Tuck, AB; Hota, C; Chambers, AF
      Osteopontin(OPN)-induced increase in human mammary epithelial cell invasiveness is urokinase (uPA)-dependent

      BREAST CANCER RESEARCH AND TREATMENT
    19. Stoychev, G; Kierdaszuk, B; Shugar, D
      Interaction of Escherichia coli purine nucleoside phosphorylase (PNP) withthe cationic and zwitterionic forms of the fluorescent substrate N(7)-methylguanosine

      BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
    20. Anspach, FB
      Endotoxin removal by affinity sorbents

      JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
    21. Zhang, XD; Guan, W; Sun, JY; Liu, QT
      Ternary complexes of cadmium(II) with phenylalanine and phenylalkylmalonates in dioxane-water mixtures

      JOURNAL OF SOLUTION CHEMISTRY
    22. Patrick, SM; Kim, S; Braunstein, NS; Maldarelli, CM; Thomas, JL; Leonard, EF
      Controlled cell deformation produces defined areas of contact between cells and ligand-coated surfaces

      ANNALS OF BIOMEDICAL ENGINEERING
    23. Cotter, RL; Zheng, JL; Che, M; Niemann, D; Liu, Y; He, J; Thomas, E; Gendelman, HE
      Regulation of human immunodeficiency virus type 1 infection, beta-chemokine production, and CCR5 expression in CD40L-stimulated macrophages: Immune control of viral entry

      JOURNAL OF VIROLOGY
    24. Urayama, S; Kawakami, A; Nakashima, T; Yamasaki, S; Hida, A; Ida, H; Kamachi, M; Nakamura, H; Origuchi, T; Migita, K; Kawabe, Y; Eguchi, K
      New disease-modifying antirheumatic drug 2-acetylthiomethyl-4-(4-methylphenyl)-4-oxobutanoic acid (KE-298) selectively augments activation-induced T cell death

      JOURNAL OF LABORATORY AND CLINICAL MEDICINE
    25. Padigel, UM; Perrin, PJ; Farrell, JP
      The development of a Th1-type response and resistance to Leishmania major infection in the absence of CD40-CD40L costimulation

      JOURNAL OF IMMUNOLOGY
    26. Taylor, L; Schwarz, H
      Identification of a soluble OX40 isoform: development of a specific and quantitative immunoassay

      JOURNAL OF IMMUNOLOGICAL METHODS
    27. Kjoller, L; Hall, A
      Rac mediates cytoskeletal rearrangements and increased cell motility induced by urokinase-type plasminogen activator receptor binding to vitronectin

      JOURNAL OF CELL BIOLOGY
    28. Nishina, Y; Sato, K; Shi, RW; Setoyama, C; Miura, R; Shiga, S
      On the ligands in charge-transfer complexes of porcine kidney flavoenzyme D-amino acid oxidase in three redox states: A resonance Raman study

      JOURNAL OF BIOCHEMISTRY
    29. Hayward, AR; Cosyns, M; Jones, M; Ponnuraj, EM
      Marrow-derived CD40-positive cells are required for mice to clear Cryptosporidium parvum infection

      INFECTION AND IMMUNITY
    30. Dono, M; Zupo, S; Massara, R; Ferrini, S; Melagrana, A; Chiorazzi, N; Ferrarini, M
      In vitro stimulation of human tonsillar subepithelial B cells: requirementfor interaction with activated T cells

      EUROPEAN JOURNAL OF IMMUNOLOGY
    31. Shinozaki, K; Yasui, K; Agematsu, K
      Direct B/B-cell interactions in immunoglobulin synthesis

      CLINICAL AND EXPERIMENTAL IMMUNOLOGY
    32. Amakata, Y; Fujiyama, Y; Andoh, A; Hodohara, K; Bamba, T
      Mechanism of NK cell activation induced by coculture with dendritic cells derived from peripheral blood monocytes

      CLINICAL AND EXPERIMENTAL IMMUNOLOGY
    33. Kotani, A; Ishikawa, T; Matsumura, Y; Ichinohe, T; Ohno, H; Hori, T; Uchiyama, T
      Correlation of peripheral blood OX40(+)(CD134(+)) T cells with chronic graft-versus-host disease in patients who underwent allogeneic hematopoietic stem cell transplantation

      BLOOD
    34. Nishigaki, T; Zamudio, FZ; Possani, LD; Darszon, A
      Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    35. Altin, JG; White, FAJ; Easton, CJ
      Synthesis of the chelator lipid nitrilotriacetic acid ditetradecylamine (NTA-DTDA) and its use with the IAsys biosensor to study receptor-ligand interactions on model membranes

      BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
    36. Gelb, MH; Min, JH; Jain, MK
      Do membrane-bound enzymes access their substrates from the membrane or aqueous phase: interfacial versus non-interfacial enzymes

      BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
    37. Arena, G; Contino, A; Fujimoto, T; Sciotto, D; Aoyama, Y
      H-1 NMR and calorimetric studies of the inclusion of trimethylammonium cations into water soluble calixresorcinarenes

      SUPRAMOLECULAR CHEMISTRY
    38. Foey, AD; Feldmann, M; Brennan, FM
      Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression

      CYTOKINE
    39. Hino, A; Igarashi, O; Tagawa, Y; Iwakura, Y; Nariuchi, H
      Interferon gamma priming is not critical for IL-12 production of murine spleen cells

      CYTOKINE
    40. Ha, CE; Petersen, CE; Park, DS; Harohalli, K; Bhagavan, NV
      Investigations of the effects of ethanol on warfarin binding to human serum albumin

      JOURNAL OF BIOMEDICAL SCIENCE
    41. van Raaij, MJ; Chouin, E; van der Zandt, H; Bergelson, JM; Cusack, S
      Dimeric structure of the coxsackievirus and adenovirus receptor D1 domain at 1.7 angstrom resolution

      STRUCTURE
    42. Whitmire, JK; Murali-Krishna, K; Altman, J; Ahmed, R
      Antiviral CD4 and CD8 T-cell memory: differences in the size of the response and activation requirements

      PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
    43. Brezinschek, HP; Dorner, T; Monson, NL; Brezinschek, RI; Lipsky, PE
      The influence of CD40-CD154 interactions on the expressed human V-H repertoire: analysis of V-H genes expressed by individual B cells of a patient with X-linked hyper-IgM syndrome

      INTERNATIONAL IMMUNOLOGY
    44. Kirschstein, O; Sip, M; Kittler, L
      Quantitative and sequence-specific analysis of DNA-ligand interaction by means of fluorescent intercalator probes

      JOURNAL OF MOLECULAR RECOGNITION
    45. Herrero, LA; Terron, A
      Interactions in solution of calcium(II) and copper(II) with nucleoside monophosphates: a calorimetric study

      JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
    46. Hakkinen, T; Karkola, K; Yla-Herttuala, S
      Macrophages, smooth muscle cells, endothelial cells, and T-cells express CD40 and CD40L in fatty streaks and more advanced human atherosclerotic lesions - Colocalization with epitopes of oxidized low-density lipoprotein, scavenger receptor, and CD16 (Fc gamma RIII)

      VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY
    47. Rarey, M; Lengauer, T
      A recursive algorithm for efficient combinatorial library docking

      PERSPECTIVES IN DRUG DISCOVERY AND DESIGN
    48. Klaus, W; Ross, A; Gsell, B; Senn, H
      Letter to the Editor: Backbone resonance assignment of the N-terminal 24 kDa fragment of the gyrase B subunit from S-aureus complexed with novobiocin

      JOURNAL OF BIOMOLECULAR NMR
    49. Taylor, JS; Burnett, RM
      DARWIN: A program for docking flexible molecules

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    50. Ma, XJ; Montaner, LJ
      Proinflammatory response and IL-12 expression in HIV-1 infection

      JOURNAL OF LEUKOCYTE BIOLOGY
    51. Clarke, SRM
      The critical role of CD40/CD40L in the CD4-dependent generation of CD8(+) T cell immunity

      JOURNAL OF LEUKOCYTE BIOLOGY
    52. Jyothi, MD; Khar, A
      Regulation of CD40L expression on natural killer cells by interleukin-12 and interferon gamma: its role in the elicitation of an effective antitumor immune response

      CANCER IMMUNOLOGY IMMUNOTHERAPY
    53. Shan, DM; Ledbetter, JA; Press, OW
      Signaling events involved in anti-CD20-induced apoptosis of malignant human B cells

      CANCER IMMUNOLOGY IMMUNOTHERAPY
    54. Lau, HKF; Teitel, JM; Kim, M
      Isolation and characterization of cell lines with reduced urokinase binding

      CLINICAL & EXPERIMENTAL METASTASIS
    55. Thoumine, O; Kocian, P; Kottelat, A; Meister, JJ
      Short-term binding of fibroblasts to fibronectin: optical tweezers experiments and probabilistic analysis

      EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
    56. Thoumine, O; Meister, JJ
      Dynamics of adhesive rupture between fibroblasts and fibronectin: microplate manipulations and deterministic model

      EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
    57. Nielsen, AD; Borch, K; Westh, P
      Thermochemistry of the specific binding of C12 surfactants to bovine serumalbumin

      BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
    58. Yawalkar, N; Egli, F; Brand, CU; Pichler, WJ; Braathen, LR
      Antigen-presenting cells and keratinocytes express interleukin-12 in allergic contact dermatitis

      CONTACT DERMATITIS
    59. Rooney, I; Butrovich, K; Ware, CF
      Expression of lymphotoxins and their receptor-Fc fusion proteins by baculovirus

      APOPTOSIS
    60. Gurevich, KG; Chekalina, ND; Zakharenko, OM
      Application of mathematical modeling to analysis of nerves growth factor interaction with platelets

      BIOORGANIC CHEMISTRY
    61. Rushworth, SA; Bravery, CA; Thompson, S
      High sequence homology between human and pig CD40 with conserved binding to human CD154

      TRANSPLANTATION
    62. Conato, C; Contino, A; Maccarrone, G; Magri, A; Remelli, M; Tabbi, G
      Copper(II) complexes with L-lysine and L-ornithine: is the side-chain involved in the coordination? A thermodynamic and spectroscopic study

      THERMOCHIMICA ACTA
    63. Kim, YH; Hong, JI
      Carbamate-appended Zn-porphyrin: a neutral receptor for anions

      TETRAHEDRON LETTERS
    64. Nagatomo, T; Koike, K
      Recent advances in structure, binding sites with ligands and pharmacological function of beta-adrenoceptors obtained by molecular biology and molecular modeling

      LIFE SCIENCES
    65. Weintraub, AS; Schnapp, LN; Lin, XJ; Taubman, MB
      Osteopontin deficiency in rat vascular smooth muscle cells is associated with an inability to adhere to collagen and increased apoptosis

      LABORATORY INVESTIGATION
    66. Yates, A; Bergmann, C; Van Hemmen, JL; Stark, J; Callard, R
      Cytokine-modulated regulation of helper T cell populations

      JOURNAL OF THEORETICAL BIOLOGY
    67. Tapramaz, R; Karabulut, B; Koksal, F
      EPR spectra of VO2+ and Cu2+ ions in di-ammonium D-tartrate single crystals

      JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
    68. Tissot, AC; Ciatto, C; Mittl, PRE; Grutter, MG; Pluckthun, A
      Viral escape at the molecular level explained by quantitative T-cell receptor/peptide/MHC interactions and the crystal structure of a peptide/MHC complex

      JOURNAL OF MOLECULAR BIOLOGY
    69. Quack, M; Carlberg, C
      Ligand-triggered stabilization of vitamin D receptor/retinoid X receptor heterodimer conformations on DR4-type response elements

      JOURNAL OF MOLECULAR BIOLOGY
    70. Zaiou, M; Arnold, KS; Newhouse, YM; Innerarity, TL; Weisgraber, KH; Segall, ML; Phillips, MC; Lund-Katz, S
      Apolipoprotein E-low density lipoprotein receptor interaction: influences of basic residue and amphipathic alpha-helix organization in the ligand

      JOURNAL OF LIPID RESEARCH
    71. Sampaio, EP; Oliveira, RB; Warwick-Davies, J; Neto, RBF; Griffin, GE; Shattock, RJ
      T cell-monocyte contact enhances tumor necrosis factor-alpha production inresponse to Mycobacterium leprae

      JOURNAL OF INFECTIOUS DISEASES
    72. Nguyen, VT; Benveniste, EN
      Involvement of STAT-1 and Ets family members in interferon-gamma inductionof CD40 transcription in microglia/macrophages

      JOURNAL OF BIOLOGICAL CHEMISTRY
    73. Jenkins, M; Keir, M; McCune, JM
      A membrane-bound Fas decoy receptor expressed by human thymocytes

      JOURNAL OF BIOLOGICAL CHEMISTRY
    74. Imai, Y; Matsunaga, I; Kusunose, E; Ichihara, K
      Unique heme environment at the putative distal region of hydrogen peroxide-dependent fatty acid alpha-hydroxylase from Sphingomonas paucimobilis (Peroxygenase P450(SP alpha))

      JOURNAL OF BIOCHEMISTRY
    75. Nishina, Y; Sato, K; Miura, R; Shiga, K
      Substrate recognition and activation mechanism of D-amino acid oxidase: A study using substrate analogs

      JOURNAL OF BIOCHEMISTRY
    76. Hida, A; Kawakami, A; Nakashima, T; Yamasaki, S; Sakai, H; Urayama, S; Ida, H; Nakamura, H; Migita, K; Kawabe, Y; Eguchi, K
      Nuclear factor-kappa B and caspases co-operatively regulate the activationand apoptosis of human macrophages

      IMMUNOLOGY
    77. Kitazawa, T; Streilein, JW
      Studies on delayed systemic effects of ultraviolet B radiation on the induction of contact hypersensitivity, 3. Dendritic cells from secondary lymphoid organs are deficient in interleukin-12 production and capacity to promote activation and differentiation of T helper type 1 cells

      IMMUNOLOGY
    78. O'Flaherty, E; Wong, WK; Pettit, SJ; Seymour, K; Ali, S; Kirby, JA
      Regulation of T-cell apoptosis: a mixed lymphocyte reaction model

      IMMUNOLOGY
    79. Egea, PF; Klaholz, BP; Moras, D
      Ligand-protein interactions in nuclear receptors of hormones

      FEBS LETTERS
    80. Lucke, C; Zhang, FL; Hamilton, JA; Sacchettini, JC; Ruterjans, H
      Solution structure of ileal lipid binding protein in complex with glycocholate

      EUROPEAN JOURNAL OF BIOCHEMISTRY
    81. Wu, Y; Denhardt, DT; Rittling, SR
      Osteopontin is required for full expression of the transformed phenotype by the ras oncogene

      BRITISH JOURNAL OF CANCER
    82. Barry, ST; Ludbrook, SB; Murrison, E; Horgan, CMT
      A regulated interaction between alpha 5 beta 1 integrin and osteopontin

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    83. Ramstad, T; Lawnicki, L; Vetto, J; Weinberg, A
      Immunohistochemical analysis of primary breast tumors and tumor draining lymph nodes by means of the T-cell costimulatory molecule OX-40

      AMERICAN JOURNAL OF SURGERY
    84. Schonbeck, U; Mach, F; Sukhova, GK; Herman, M; Graber, P; Kehry, MR; Libby, P
      CD40 ligation induces tissue factor expression in human vascular smooth muscle cells

      AMERICAN JOURNAL OF PATHOLOGY
    85. Bielawski, K; Bielawska, A; Bartulewicz, D; Rozanski, A
      Molecular modelling of the interaction of carbocyclic analogues of netropsin and distamycin with d(CGCGAATTCGCG)(2)

      ACTA BIOCHIMICA POLONICA
    86. Frucht, DM; Sandberg, DI; Brown, MR; Gerstberger, SM; Holland, SM
      IL-12 independent costimulation pathways for interferon-gamma production in familial dissemination Mycobacterium avium complex infection

      CLINICAL IMMUNOLOGY
    87. Stuber, E; von Freier, A; Folsch, UR
      The effect of anti-gp39 treatment on the intestinal manifestations of acute murine graft-versus-host disease

      CLINICAL IMMUNOLOGY
    88. Gonzalez, M; Argarana, CE; Fidelio, GD
      Extremely high thermal stability of streptavidin and avidin upon biotin binding

      BIOMOLECULAR ENGINEERING
    89. Posner, MR; Cavacini, LA; Upton, MP; Tillman, KC; Gornstein, ER; Norris, CM
      Surface membrane-expressed CD40 is present on tumor cells from squamous cell cancer of the head and neck in vitro and in vivo and regulates cell growth in tumor cell lines

      CLINICAL CANCER RESEARCH
    90. Isler, P; de Rochemonteix, BG; Songeon, F; Boehringer, N; Nicod, LP
      Interleukin-12 production by human alveolar macrophages is controlled by the autocrine production of interleukin-10

      AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
    91. Gimsa, U; Mitchison, A; Allen, R
      Inhibitors of Src-family tyrosine kinases favour Th2 differentiation

      CYTOKINE
    92. Heath, WR; Carbone, FR
      Cytotoxic T lymphocyte activation by cross-priming

      CURRENT OPINION IN IMMUNOLOGY
    93. Nagasaki, T; Ishimura, E; Koyama, H; Shioi, A; Jono, S; Inaba, M; Hasuma, T; Yokoyama, M; Nishizawa, E; Morii, H; Otani, S
      alpha v integrin regulates TNF-alpha-induced nitric oxide synthesis in ratmesangial cells - possible role of osteopontin

      NEPHROLOGY DIALYSIS TRANSPLANTATION
    94. Liu, M; Wang, SM
      MCDOCK: A Monte Carlo simulation approach to the molecular docking problem

      JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN
    95. Nishimoto, E; Yamashita, S; Yamasaki, N; Imoto, T
      Resolution and characterization of tryptophyl fluorescence of hen egg-white lysozyme by quenching- and time-resolved spectroscopy

      BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
    96. Kramer, B; Rarey, M; Lengauer, T
      Evaluation of the FLEXX incremental construction algorithm for protein-ligand docking

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    97. Rao, MS; Olson, AJ
      Modelling of Factor Xa-inhibitor complexes: A computational flexible docking approach

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    98. Rarey, M; Kramer, B; Lengauer, T
      The particle concept: Placing discrete water molecules during protein-ligand docking predictions

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    99. NANDAGOPAL K; TERZAGHIHOWE M; NIYOGI SK
      RECEPTOR RECOGNITION BY HISTIDINE 16 OF HUMAN EPIDERMAL GROWTH-FACTORVIA HYDROGEN-BOND DONOR ACCEPTOR INTERACTIONS/

      Journal of cellular biochemistry
    100. Brand, CU; Hunger, RE; Yawalkar, N; Gerber, HA; Schaffner, T; Braathen, LR
      Characterization of human skin-derived CD1a-positive lymph cells

      ARCHIVES OF DERMATOLOGICAL RESEARCH


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Documento generato il 30/05/20 alle ore 19:49:21