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La ricerca find articoli where soggetti phrase all words 'ACTIVE-SITE' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 6069 riferimenti
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    1. Castro, C; Britt, BM
      Binding thermodynamics of the transition state analogue coformycin and of the ground state analogue 1-deazaadenosine to bovine adenosine deaminase

      JOURNAL OF ENZYME INHIBITION
    2. Salvati, L; Mattu, M; Tiberi, F; Polticelli, F; Ascenzi, P
      Inhibition of Saccharomyces cerevisiae phosphomannose isomerase by the NO-donor S-nitroso-acetyl-penicillamine

      JOURNAL OF ENZYME INHIBITION
    3. Federico, R; Leone, L; Botta, M; Binda, C; Angelini, R; Venturini, G; Ascenzi, P
      Inhibition of pig liver and Zea mays L. polyamine oxidase: A comparative study

      JOURNAL OF ENZYME INHIBITION
    4. Gadda, G; Banerjee, A; Fleming, GS; Fitzpatrick, PF
      Evidence for an essential arginine in the flavoprotein nitroalkane oxidase

      JOURNAL OF ENZYME INHIBITION
    5. Ma, YF; Evans, DE; Logue, SJ; Langridge, P
      Mutations of barley beta-amylase that improve substrate-binding affinity and thermostability

      MOLECULAR GENETICS AND GENOMICS
    6. Sarto, C; Deon, C; Doro, G; Hochstrasser, DF; Mocarelli, P; Sanchez, JC
      Contribution of proteomics to the molecular analysis of renal cell carcinoma with an emphasis on manganese superoxide dismutase

      PROTEOMICS
    7. Campillo, N; Nagarajaram, HA; Ghosh, I
      Phosphoribosyltransferase superfamily: A comparative structural analysis

      JOURNAL OF MOLECULAR MODELING
    8. Baffert, C; Chen, HG; Crabtree, RH; Brudvig, GW; Collomb, MN
      Electrochemical properties of [Mn-III(terpy)(N-3)(3)] (terpy=2,2 ': 6 ',2 ''-terpyridine) in CH3CN - Electrogeneration of dimanganese(II) di-mu-azidoand dimanganese(IV) di-mu-oxo complexes

      JOURNAL OF ELECTROANALYTICAL CHEMISTRY
    9. Gieselman, MD; Xie, LL; van der Donk, WA
      Synthesis of a selenocysteine-containing peptide by native chemical ligation

      ORGANIC LETTERS
    10. Zhang, BL; Cui, ZY; Sun, LL
      Synthesis of 5 '-deoxy-5 '-thioguanosine-5 '-monophosphorothioate and its incorporation into RNA 5 '-termini

      ORGANIC LETTERS
    11. Elcock, AH; Sept, D; McCammon, JA
      Computer simulation of protein-protein interactions

      JOURNAL OF PHYSICAL CHEMISTRY B
    12. Floyd, JS; Haralampus-Grynaviski, N; Ye, T; Zheng, B; Simon, JD; Edington, MD
      Time-resolved spectroscopic studies of radiationless decay processes in photoexcited hemocyanins

      JOURNAL OF PHYSICAL CHEMISTRY B
    13. Yoda, M; Houjou, H; Inoue, Y; Sakurai, M
      Spectral tuning of photoactive yellow protein. Theoretical and experimental analysis of medium effects on the absorption spectrum of the chromophore

      JOURNAL OF PHYSICAL CHEMISTRY B
    14. Kangas, E; Tidor, B
      Electrostatic complementarity at ligand binding sites: Application to chorismate mutase

      JOURNAL OF PHYSICAL CHEMISTRY B
    15. Worthington, SE; Roitberg, AE; Krauss, M
      An MD/QM study of the chorismate mutase-catalyzed Claisen rearrangement reaction

      JOURNAL OF PHYSICAL CHEMISTRY B
    16. Rocchia, W; Alexov, E; Honig, B
      Extending the applicability of the nonlinear Poisson-Boltzmann equation: Multiple dielectric constants and multivalent ions

      JOURNAL OF PHYSICAL CHEMISTRY B
    17. Isaev, A; Scheiner, S
      Proton conduction by a chain of water molecules in carbonic anhydrase

      JOURNAL OF PHYSICAL CHEMISTRY B
    18. Prabhakar, R; Siegbahn, PEM
      A theoretical study of the mechanism for the reductive half-reaction of pea seedling amine oxidase (PSAO)

      JOURNAL OF PHYSICAL CHEMISTRY B
    19. Torrent, M; Musaev, DG; Morokuma, K; Basch, H
      A density functional study of possible intermediates of the reaction of dioxygen molecule with nonheme iron complexes. 2. "Water-assisted" model studies

      JOURNAL OF PHYSICAL CHEMISTRY B
    20. Torrent, M; Musaev, DG; Morokuma, K
      The flexibility of carboxylate ligands in methane monooxygenase and ribonucleotide reductase: A density functional study

      JOURNAL OF PHYSICAL CHEMISTRY B
    21. Bennett, DJ; Buchanan, KI; Cooke, A; Epemolu, O; Hamilton, NM; Hutchinson, EJ; Mitchell, A
      Enantiospecific enzyme-catalysed resolution of novel N,N-disubstituted alpha-amino acid phenolic ester derivatives using pig liver esterase

      JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1
    22. Yin, LH; Cheng, P; Yan, SP; Li, XQ; Li, J; Liao, DZ; Jiang, ZH
      The first iron(III)-nickel(II) heterodinuclear complex containing both terminal and bridged phosphato ligands relevant to structural core models for dimetalloenzymes

      JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS
    23. Gou, SH; Qian, M; Yu, Z; Duan, CY; Sun, XF; Huang, W
      Synthesis, molecular structure and magnetic properties of tetranuclear copper(II) complexes with pendant-arm macrocyclic ligands

      JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS
    24. Sevier, CS; Cuozzo, JW; Vala, A; Aslund, F; Kaiser, CA
      A flavoprotein oxidase defines a new endoplasmic reticulum pathway for biosynthetic disulphide bond formation

      NATURE CELL BIOLOGY
    25. Stein, M; Lubitz, W
      The electronic structure of the catalytic intermediate Ni-C in [NiFe] and [NiFeSe] hydrogenases

      PHYSICAL CHEMISTRY CHEMICAL PHYSICS
    26. Stein, M; Lubitz, W
      DFT calculations of the electronic structure of the paramagnetic states Ni-A, Ni-B and Ni-C of [NiFe] hydrogenase

      PHYSICAL CHEMISTRY CHEMICAL PHYSICS
    27. Stewart, LJ; Bailey, S; Collison, D; Morris, GA; Preece, I; Garner, CD
      In vivo oxo transfer: Reactions of native and W-substituted dimethyl sulfoxide reductase monitored by H-1 NMR spectroscopy

      CHEMBIOCHEM
    28. Muller, BH; Lamoure, C; Le Du, MH; Cattolico, L; Lajeunesse, E; Lemaitre, F; Pearson, A; Ducancel, F; Menez, A; Boulain, JC
      Improving Escherichia coli alkaline phosphatase efficacy by additional mutations inside and outside the catalytic pocket

      CHEMBIOCHEM
    29. Gomes, CM; Le Gall, J; Xavier, AV; Teixeira, M
      Could a diiron-containing four-helix-bundle protein have been a primitive oxygen reductase?

      CHEMBIOCHEM
    30. Stauffer, F; Zizzari, E; Engeloch-Jarret, C; Faurite, JP; Bobalova, J; Neier, R
      Inhibition studies of porphobilinogen synthase from Escherichia coli differentiating between the two recognition sites

      CHEMBIOCHEM
    31. Mauksch, M; Brauer, M; Weston, J; Anders, E
      New insights into the mechanistic details of the carbonic anhydrase cycle as derived from the model system [(NH3)(3)Zn(OH)](+)/CO2: How does the H2O/HCO(3)(-)replacement step occur?

      CHEMBIOCHEM
    32. Brakmann, S
      Discovery of superior enzymes by directed molecular evolution

      CHEMBIOCHEM
    33. Ochs, C; Hahn, FE; Frohlich, R
      Coordination chemistry of unsymmetrical tripodal ligands with an NNO2 donor set

      EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
    34. Albedyhl, S; Averbuch-Pouchot, MT; Belle, C; Krebs, B; Pierre, JL; Saint-Aman, E; Torelli, S
      Dinuclear zinc(II)-iron(III) and iron(II)-iron(III) complexes as models for purple acid phosphatases

      EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
    35. Tesmer, M; Vahrenkamp, H
      Sterically fixed dithiolate ligands and their zinc complexes: Derivatives of 1,8-dimercaptonaphthalene

      EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
    36. Wang, DR; Ebel, M; Schulzke, C; Gruning, C; Hazari, SKS; Rehder, D
      Vanadium(IV and V) complexes containing SNO (dithiocarbonylhydrazone; thiosemicarbazone) donor sets

      EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
    37. Visnjevac, A; Tusek-Bozic, L; Majeric-Elenkov, M; Sunjic, V; Kojic-Prodic, B
      Copper(II)-promoted chemical transformations of 3-substituted 5-(2 '-pyridyl)-1,4-benzodiazepin-2-one derivatives. Crystal structures and spectroscopic characterisation of metal complexes

      EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
    38. Padiglia, A; Medda, R; Bellelli, A; Agostinelli, E; Morpurgo, L; Mondovi, B; Agro, AF; Floris, G
      The reductive and oxidative half-reactions and the role of copper ions in plant and mammalian copper-amine oxidases

      EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
    39. Didier, A; Michaudet, L; Ricard, D; Baveux-Chambenoit, V; Richard, P; Boitrel, B
      A versatile and convenient method for the functionalization of porphyrins

      EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
    40. Cabaret, D; Gonzalez, MG; Wakselman, M; Adediran, SA; Pratt, RF
      Synthesis, hydrolysis, and evaluation of 3-acylamino-3,4-dihydro-2-oxo-2H-1,3-benzoxazinecarboxylic acids and linear azadepsipeptides as potential substrates/inhibitors of beta-lactam-recognizing enzymes

      EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
    41. Strasdeit, H
      The first cadmium-specific enzyme

      ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
    42. Jiang, JF; Koch, SA
      trans-[Fe(CN)(4)(CO)(2)](2-), a 21st century [Fe(CN)(CO)] compound

      ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
    43. Topf, M; Varnai, P; Richards, WG
      Quantum mechanical/molecular mechanical study of three stationary points along the deacylation step of the catalytic mechanism of elastase

      THEORETICAL CHEMISTRY ACCOUNTS
    44. Schurer, H; Schiffer, S; Marchfelder, A; Morl, M
      This is the end: Processing, editing and repair at the tRNA 3 '-terminus

      BIOLOGICAL CHEMISTRY
    45. Barrett, AJ; Rawlings, ND
      Evolutionary lines of cysteine peptidases

      BIOLOGICAL CHEMISTRY
    46. Hofmann, B; Budde, H; Bruns, K; Guerrero, SA; Kalisz, HM; Menge, U; Montemartini, M; Nogoceke, E; Steinert, P; Wissing, JB; Flohe, L; Hecht, HJ
      Structures of tryparedoxins revealing interaction with trypanothione

      BIOLOGICAL CHEMISTRY
    47. Stroppolo, ME; Falconi, M; Caccuri, AM; Desideri, A
      Superefficient enzymes

      CELLULAR AND MOLECULAR LIFE SCIENCES
    48. Chatani, E; Hayashi, R
      Functional and structural roles of constituent amino acid residues of bovine pancreatic ribonuclease A

      JOURNAL OF BIOSCIENCE AND BIOENGINEERING
    49. Ghanevati, M; Jaworski, JC
      Active-site residues of a plant membrane-bound fatty acid elongase beta-ketoacyl-CoA synthase, FAE1 KCS

      BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
    50. Satoh, K; Hayakari, M; Ookawa, K; Satou, M; Aizawa, S; Tanaka, M; Hatayama, I; Tsuchida, S; Uchida, K
      Lipid peroxidation end products-responded induction of a preneoplastic marker enzyme glutathione S-transferase P-form (GST-P) in rat liver on admistration via the portal vein

      MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
    51. Srivastava, PK; Kayastha, AM
      Studies on the histidine residues in pigeonpea (Cajanus cajan L.) urease

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    52. Wong, E; Sim, J; Sim, TS
      Construction of Streptomyces jumonjinensis isopenicillin N synthase doublemutants, Ser119Ala/Glu120Gly and Thr308Ala/Thr309Val, overcomes insoluble expression in Escherichia coli

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    53. Moracci, M; Trincone, A; Rossi, M
      Glycosynthases: new enzymes for oligosaccharide synthesis

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    54. Bossi, L; D'Arrigo, P; Pedrocchi-Fantoni, G; Mele, A; Servi, S; Leiros, I
      The substrate requirements of phospholipase D

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    55. Cameselle, JC; Agudo, A; Canales, J; Costas, MJ; Fernandez, A; Flores, A; Garcia-Diaz, M; Gonzalez-Santiago, S; Lopez-Gomez, J; Ribeiro, JM; Vergeles, JM
      Nucleotide ester-forming alcoholytic activities of nucleotide pyrophosphatases: implications for practical biotransformation, enzyme mechanisms and biological function

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    56. Borreguero, I; Sanchez-Montero, JM; Sinisterra, JV; Rumbero, A; Hermoso, JA; Alcantara, AR
      Regioselective resolution of 1,n-diols catalysed by lipases: a rational explanation of the enzymatic selectivity

      JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
    57. Guliants, VV; Holmes, SA
      Probing polyfunctional nature of vanadyl pyrophosphate catalysts: oxidation of 16 C-4 molecules

      JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
    58. Guliants, VV; Holmes, SA; Benziger, JB; Heaney, P; Yates, D; Wachs, IE
      In situ studies of atomic, nano- and macroscale order during VOHPO4 centerdot 0.5H(2)O transformation to (VO)(2)P2O7

      JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
    59. Richard, JP; Amyes, TL
      Proton transfer at carbon

      CURRENT OPINION IN CHEMICAL BIOLOGY
    60. Zechel, DL; Withers, SG
      Dissection of nucleophilic and acid-base catalysis in glycosidases

      CURRENT OPINION IN CHEMICAL BIOLOGY
    61. Poppe, L
      Methylidene-imidazolone: a novel electrophile for substrate activation

      CURRENT OPINION IN CHEMICAL BIOLOGY
    62. Razavet, M; Davies, SC; Hughes, DL; Pickett, CJ
      {2Fe3S} clusters related to the di-iron sub-site of the H-centre of all-iron hydrogenases

      CHEMICAL COMMUNICATIONS
    63. Benisvy, L; Blake, AJ; Collison, D; Davies, ES; Garner, CD; McInnes, EJL; McMaster, J; Whittaker, G; Wilson, C
      A phenoxyl radical complex of copper(II)

      CHEMICAL COMMUNICATIONS
    64. Kersting, B; Steinfeld, G
      The effect of N-methylation on the chemical reactivity of binuclear Ni amine-thiophenolate complexes

      CHEMICAL COMMUNICATIONS
    65. Bugg, TDH; Lin, G
      Solving the riddle of the intradiol and extradiol catechol dioxygenases: how do enzymes control hydroperoxide rearrangements?

      CHEMICAL COMMUNICATIONS
    66. Casny, M; Rehder, D
      Towards hydroperoxovanadium complexes: the X-ray crystal structure of a peroxovanadium(v) complex containing a V(O-2)(RCO2H)(H2O)(2) cluster with hydrogen bond inter-linkages

      CHEMICAL COMMUNICATIONS
    67. Yuan, ZY; Trias, J; White, RJ
      Deformylase as a novel antibacterial target

      DRUG DISCOVERY TODAY
    68. Kazan, D; Erarslan, A
      Identification of catalytically essential amino acid residues of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105

      PROCESS BIOCHEMISTRY
    69. Chaufan, G; de Molina, MDR; de Viale, LCS
      How does hexachlorobenzene treatment affect liver uroporphyrinogen decarboxylase?

      INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
    70. Ikawa, Y; Yoshioka, W; Ohki, Y; Shiraishi, H; Inoue, T
      Self-splicing of the Tetrahymena group I ribozyme without conserved base-triples

      GENES TO CELLS
    71. Heide, C; Feltens, R; Hartmann, RK
      Purine N7 groups that are crucial to the interaction of Escherichia coli RNase P RNA with tRNA

      RNA-A PUBLICATION OF THE RNA SOCIETY
    72. Alvi, RK; Lund, M; O'Keefe, RT
      ATP-dependent interaction of yeast U5 snRNA loop 1 with the 5 ' splice site

      RNA-A PUBLICATION OF THE RNA SOCIETY
    73. Alvarez, CJ; Wise, JA
      Activation of a cryptic 5 ' splice site by U1 snRNA

      RNA-A PUBLICATION OF THE RNA SOCIETY
    74. Tuschl, T; Sharp, PA; Bartel, DP
      A ribozyme selected from variants of U6 snRNA promotes 2 ',5 '-branch formation

      RNA-A PUBLICATION OF THE RNA SOCIETY
    75. Higa, K; Gao, C; Motokawa, W; Abe, K
      The roles of the N-terminal portions of various tachykinins in promoting salivation

      ORAL DISEASES
    76. Neamati, N
      Structure-based HIV-1 integrase inhibitor design: a future perspective

      EXPERT OPINION ON INVESTIGATIONAL DRUGS
    77. Supuran, CT; Scozzafava, A; Mastrolorenzo, A
      Bacterial proteases: current therapeutic use and future prospects for the development of new antibiotics

      EXPERT OPINION ON THERAPEUTIC PATENTS
    78. Ludemann, SK; Gabdoulline, RR; Lounnas, V; Wade, RC
      Substrate access to cytochrome P450cam investigated by molecular dynamics simulations: An interactive look at the underlying mechanisms

      INTERNET JOURNAL OF CHEMISTRY
    79. Fugmann, SD; Schatz, DG
      Identification of basic residues in RAG2 critical for DNA binding by the RAG1-RAG2 complex

      MOLECULAR CELL
    80. de Zamaroczy, M; Mora, L; Lecuyer, A; Geli, V; Buckingham, RH
      Cleavage of colicin D is necessary for cell killing and requires the innermembrane peptidase LepB

      MOLECULAR CELL
    81. Frilander, MJ; Steitz, JA
      Dynamic exchanges of RNA interactions leading to catalytic core formation in the U12-dependent spliceosome

      MOLECULAR CELL
    82. Kishimura, H; Ojima, T; Hayashi, K; Nishita, K
      Bacterial expression and characterization of starfish phospholipase A(2)

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
    83. Meijer, WJJ; Horcajadas, JA; Salas, M
      phi 29 family of phages

      MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
    84. Felder, C; Jiang, HL; Zhu, WL; Chen, KX; Silman, I; Botti, SA; Sussman, JL
      Quantum/classical mechanical comparison of cation-pi interactions between tetramethylammonium and benzene

      JOURNAL OF PHYSICAL CHEMISTRY A
    85. Zhou, Y; Ujj, L; Meyer, TE; Cusanovich, MA; Atkinson, GH
      Photocycle dynamics and vibrational spectroscopy of the E46Q mutant of photoactive yellow protein

      JOURNAL OF PHYSICAL CHEMISTRY A
    86. Oliva, M; Safont, VS; Andres, J; Tapia, O
      Transition structures for D-ribulose-1,5-bisphosphate carboxylase/oxygenase-catalyzed oxygenation chemistry: Role of carbamylated lysine in a model magnesium coordination sphere

      JOURNAL OF PHYSICAL CHEMISTRY A
    87. Malik, HS; Eickbush, TH
      Phylogenetic analysis of ribonuclease H domains suggests a late, chimeric origin of LTR retrotransposable elements and retroviruses

      GENOME RESEARCH
    88. Srivastava, P; Mukherjee, KJ
      Cloning, characterization, and expression of xylanase gene from Bacillus lyticus in Escherichia coli and Bacillus subtilis

      PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
    89. Lupidi, G; Venardi, G; Bollettini, M; Marmocchi, F; Rotilo, G
      Purification and partial characterization of glyoxalase I from bovine brain

      PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
    90. Pessi, A
      A personal account of the role sf peptide research in drug discovery: the case of hepatitis C

      JOURNAL OF PEPTIDE SCIENCE
    91. Kessler, BM; Tortorella, D; Altun, M; Kisselev, AF; Fiebiger, E; Hekking, BG; Ploegh, HL; Overkleeft, HS
      Extended peptide-based inhibitors efficiently target the proteasome and reveal overlapping specificities of the catalytic beta-subunits

      CHEMISTRY & BIOLOGY
    92. Walther, M; Ivanov, I; Myagkova, G; Kuhn, H
      Alterations of lipoxygenase specificity by targeted substrate modificationand site-directed mutagenesis

      CHEMISTRY & BIOLOGY
    93. Boehr, DD; Lane, WS; Wright, GD
      Active site labeling of the gentamicin resistance enzyme AAQ6 ')-APH(2 '')by the lipid kinase inhibitor wortmannin

      CHEMISTRY & BIOLOGY
    94. Fritz, TA; Tondi, D; Finer-Moore, JS; Costi, MP; Stroud, RM
      Predicting and harnessing protein flexibility in the design of species-specific inhibitors of thymidylate synthase

      CHEMISTRY & BIOLOGY
    95. Caselli, E; Powers, RA; Blasczcak, LC; Wu, CYE; Prati, F; Shoichet, BK
      Energetic, structural, and antimicrobial analyses of beta-lactam side chain recognition by beta-lactamases

      CHEMISTRY & BIOLOGY
    96. Remaut, H; Bompard-Gilles, C; Goffin, C; Frere, JM; Van Beeumen, J
      Structure of the Bacillus subtilis D-aminopeptidase DppA reveals a novel self-compartmentalizing protease

      NATURE STRUCTURAL BIOLOGY
    97. Rice, P; Baker, TA
      Comparative architecture of transposase and integrase complexes (vol 8, pg302, 2001)

      NATURE STRUCTURAL BIOLOGY
    98. Rice, PA; Baker, TA
      Comparative architecture of transposase and integrase complexes

      NATURE STRUCTURAL BIOLOGY
    99. Doherty, EA; Batey, RT; Masquida, B; Doudna, JA
      A universal mode of helix packing in RNA

      NATURE STRUCTURAL BIOLOGY
    100. Brudler, R; Rammelsberg, R; Woo, TT; Getzoff, ED; Gerwert, K
      Structure of the I-1 early intermediate of photoactive yellow protein by FTIR spectroscopy

      NATURE STRUCTURAL BIOLOGY


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Documento generato il 16/02/20 alle ore 20:39:47