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La ricerca find articoli where soggetti phrase all words 'ACTIVATION DOMAINS' sort by level,fasc_key/DESCEND, pagina_ini_num/ASCEND ha restituito 243 riferimenti
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    1. Marmorstein, R
      Protein modules that manipulate histone tails for chromatin regulation

      NATURE REVIEWS MOLECULAR CELL BIOLOGY
    2. Gollner, H; Bouwman, P; Mangold, M; Karis, A; Braun, H; Rohner, I; Del Rey, A; Besedovsky, HO; Meinhardt, A; van den Broek, M; Cutforth, T; Grosveld, F; Philipsen, S; Suske, G
      Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene

      GENES TO CELLS
    3. Puustinen, R; Sarvilinna, N; Manninen, T; Tuohimaa, P; Ylikomi, T
      Localization of glucocorticoid receptor interacting protein 1 in murine tissues using two novel polyclonal antibodies

      EUROPEAN JOURNAL OF ENDOCRINOLOGY
    4. Goldstrohm, AC; Greenleaf, AL; Garcia-Blanco, MA
      Co-transcriptional splicing of pre-messenger RNAs: considerations for the mechanism of alternative splicing

      GENE
    5. Goldstrohm, AC; Albrecht, TR; Sune, C; Bedford, MT; Garcia-Blanco, MA
      The transcription elongation factor CA150 interacts with RNA polymerase IIand the pre-mRNA splicing factor SF1

      MOLECULAR AND CELLULAR BIOLOGY
    6. Kadener, S; Cramer, P; Nogues, G; Cazalla, D; de la Mata, M; Fededa, JP; Werbajh, SE; Srebrow, A; Kornblihtt, AR
      Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing

      EMBO JOURNAL
    7. Rui, E; de Moura, PR; Kobarg, J
      Expression of deletion mutants of the hepatitis B virus protein HBx in E-coli and characterization of their RNA binding activities

      VIRUS RESEARCH
    8. Barak, O; Aronheim, A; Shaul, Y
      HBV X protein targets HIV tat-binding protein 1

      VIROLOGY
    9. Hirst, M; Ho, C; Sabourin, L; Rudnicki, M; Penn, L; Sadowski, I
      A two-hybrid system for transactivator bait proteins

      PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    10. Pankratova, EV; Deyev, IE; Zhenilo, SV; Anfalova, TV; Polanovsky, OL
      Isoforms of transcription factor Oct-1 synthesized in lymphocytes

      MOLECULAR BIOLOGY
    11. Pankratova, EV; Deev, IE; Polanovsky, OL
      The tissue-specific splicing of 5'-terminal exons of the Oct-1 gene

      MOLECULAR BIOLOGY
    12. Kumar, MB; Ramadoss, P; Reen, RK; Perdew, GH
      The Q-rich subdomain of the human Ah receptor transactivation domain is required for dioxin-mediated transcriptional activity

      JOURNAL OF BIOLOGICAL CHEMISTRY
    13. Nicolas, M; Noe, V; Jensen, KB; Ciudad, CJ
      Cloning and characterization of the 5 '-flanking region of the human transcription factor Sp1 gene

      JOURNAL OF BIOLOGICAL CHEMISTRY
    14. Fazio, IK; Bolger, TA; Gill, G
      Conserved regions of the Drosophila erect wing protein contribute both positively and negatively to transcriptional activity

      JOURNAL OF BIOLOGICAL CHEMISTRY
    15. Tang, JG; Koeffler, HP
      Structural and functional studies of CCAAT/enhancer-binding protein epsilon

      JOURNAL OF BIOLOGICAL CHEMISTRY
    16. Fujii, T; Brandsma, JL; Peng, XY; Srimatkandada, S; Li, L; Canaan, A; Deisseroth, AB
      High and low levels of cottontail rabbit papillomavirus E2 protein generate opposite effects on gene expression

      JOURNAL OF BIOLOGICAL CHEMISTRY
    17. Mouillet, JF; Henrich, VC; Lezzi, M; Vogtli, M
      Differential control of gene activity by isoforms A, B1 and B2 of the Drosophila ecdysone receptor

      EUROPEAN JOURNAL OF BIOCHEMISTRY
    18. Hiratani, I; Mochizuki, T; Tochimoto, N; Taira, M
      Functional domains of the LIM homeodomain protein Xlim-1 involved in negative regulation, transactivation, and axis formation in Xenopus embryos

      DEVELOPMENTAL BIOLOGY
    19. Yuan, CX; Gurley, WB
      Potential targets for HSF1 within the preinitiation complex

      CELL STRESS & CHAPERONES
    20. Sterner, DE; Berger, SL
      Acetylation of histones and transcription-related factors

      MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
    21. Matsuo, K; Owens, JM; Tonko, M; Elliott, C; Chambers, TJ; Wagner, EF
      Fosl1 is a transcriptional target of c-Fos during osteoclast differentiation

      NATURE GENETICS
    22. Sekito, T; Thornton, J; Butow, RA
      Mitochondria-to-nuclear signaling is regulated by the subcellular localization of the transcription factors Rtg1p and Rtg3p

      MOLECULAR BIOLOGY OF THE CELL
    23. Wolffe, AP; Guschin, D
      Chromatin structural features and targets that regulate transcription

      JOURNAL OF STRUCTURAL BIOLOGY
    24. Weterman, MAJ; von Groningen, JJM; Jansen, A; van Kessel, AG
      Nuclear localization and transactivating capacities of the papillary renalcell carcinoma-associated TFE3 and PRCC (fusion) proteins

      ONCOGENE
    25. Uversky, VN; Gillespie, JR; Fink, AL
      Why are "natively unfolded" proteins unstructured under physiologic conditions?

      PROTEINS-STRUCTURE FUNCTION AND GENETICS
    26. Coin, F; Egly, JM
      Formation of the transcriptional initiation complex: from general factors to chromatin remodeling complexes

      M S-MEDECINE SCIENCES
    27. Urlinger, S; Helbl, V; Guthmann, J; Pook, E; Grimm, S; Hillen, W
      The p65 domain from NF-kappa B is an efficient human activator in the tetracycline-regulatable gene expression system

      GENE
    28. Herbst, A; Tansey, WP
      HAM: A new epitope-tag for in vivo protein labeling

      MOLECULAR BIOLOGY REPORTS
    29. Giangrande, PH; Kimbrel, EA; Edwards, DP; McDonnell, DP
      The opposing transcriptional activities of the two isoforms of the human progesterone receptor are due to differential cofactor binding

      MOLECULAR AND CELLULAR BIOLOGY
    30. Escher, D; Bodmer-Glavas, M; Barberis, A; Schaffner, W
      Conservation of glutamine-rich transactivation function between yeast and humans

      MOLECULAR AND CELLULAR BIOLOGY
    31. Tsukihashi, Y; Mikaye, T; Kawaichi, M; Kokubo, T
      Impaired core promoter recognition caused by novel yeast TAF145 mutations can be restored by creating a canonical TATA element within the promoter region of the TUB2 gene

      MOLECULAR AND CELLULAR BIOLOGY
    32. Tang, HB; Liu, YY; Madabusi, L; Gilmour, DS
      Promoter-proximal pausing on the hsp70 promoter in Drosophila melanogasterdepends on the upstream regulator

      MOLECULAR AND CELLULAR BIOLOGY
    33. Huang, SM; Stallcup, MR
      Mouse Zac1, a transcriptional coactivator and repressor for nuclear receptors

      MOLECULAR AND CELLULAR BIOLOGY
    34. Belotserkovskaya, R; Sterner, DE; Deng, M; Sayre, MH; Lieberman, PM; Berger, SL
      Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8at Gcn4-activated promoters

      MOLECULAR AND CELLULAR BIOLOGY
    35. Hou, SY; Wu, SY; Zhou, TY; Thomas, MC; Chiang, CM
      Alleviation of human papillomavirus E2-mediated transcriptional repressionvia formation of a TATA binding protein (or TFIID)-TFIIB-RNA polymerase II-TFIIF preinitiation complex

      MOLECULAR AND CELLULAR BIOLOGY
    36. Li, CG; Ling, XB; Yuan, BB; Minoo, P
      A novel DNA element mediates transcription of Nkx2.1 by Sp1 and Sp3 in pulmonary epithelial cells

      BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
    37. Grondin, B; DeLuca, N
      Herpes simplex virus type 1 ICP4 promotes transcription preinitiation complex formation by enhancing the binding of TFIID to DNA

      JOURNAL OF VIROLOGY
    38. Grapes, M; O'Hare, P
      Differences in determinants required for complex formation and transactivation in related VP16 proteins

      JOURNAL OF VIROLOGY
    39. Melcher, K
      The strength of acidic activation domains correlates with their affinity for both transcriptional and non-transcriptional proteins

      JOURNAL OF MOLECULAR BIOLOGY
    40. de Stanchina, E; Gabellini, D; Norio, P; Giacca, M; Peverali, FA; Riva, S; Falaschi, A; Biamonti, G
      Selection of homeotic proteins for binding to a human DNA replication origin

      JOURNAL OF MOLECULAR BIOLOGY
    41. Brightbill, HD; Plevy, SE; Modlin, RL; Smale, ST
      A prominent role for Sp1 during lipopolysaccharide-mediated induction of the IL-10 promoter in macrophages

      JOURNAL OF IMMUNOLOGY
    42. Kim, JM; Hong, Y; Jeang, KT; Kim, S
      Transactivation activity of the human cytomegalovirus IE2 protein occurs at steps subsequent to TATA box-binding protein recruitment

      JOURNAL OF GENERAL VIROLOGY
    43. Zhang, ZS; Torii, N; Furusaka, A; Malayaman, N; Hu, ZY; Liang, TJ
      Structural and functional characterization of interaction between hepatitis B virus X protein and the proteasome complex

      JOURNAL OF BIOLOGICAL CHEMISTRY
    44. Leenders, H; Whitfield, S; Benoist, C; Mathis, D
      Role of the forkhead transcription family member, FKHR, in thymocyte differentiation

      EUROPEAN JOURNAL OF IMMUNOLOGY
    45. Kim, JM; Hong, Y; Kim, S
      Artificial recruitment of Sp1 or TBP can replace the role of IE1 in the synergistic transactivation by IE1 and IE2

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
    46. Hayashi, Y; Wang, W; Ninomiya, T; Nagano, H; Ohta, K; Otoh, H
      Liver enriched transcription factors and differentiation of hepatocellularcarcinoma

      JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY
    47. Tang, H; Sharp, PA
      Transcriptional regulation of the murine 3 ' IgH enhancer by OCT-2

      IMMUNITY
    48. Nagoshi, E; Imamoto, N; Sato, R; Yoneda, Y
      Nuclear import of sterol regulatory element-binding protein-2, a basic helix-loop-helix-leucine zipper (bHLH-Zip)-containing transcription factor, occurs through the direct interaction of importin beta with HLH-Zip

      MOLECULAR BIOLOGY OF THE CELL
    49. Leichter, M; Thiel, G
      Transcriptional repression by the zinc finger protein REST is mediated by titratable nuclear factors

      EUROPEAN JOURNAL OF NEUROSCIENCE
    50. Barbeau, B; Barat, C; Bergeron, D; Rassart, E
      The GATA-1 and Spi-1 transcriptional factors bind to a GATA/EBS dual element in the Fli-1 exon 1

      ONCOGENE
    51. Chang, CH; Scott, GK; Baldwin, MA; Benz, CC
      Exon 4-encoded acidic domain in the epithelium-restricted Ets factor, ESX,confers potent transactivating capacity and binds to TATA-binding protein (TBP)

      ONCOGENE
    52. Yang, ZH; Dickman, MB
      Colletotrichum trifolii mutants disrupted in the catalytic subunit of cAMP-dependent protein kinase are nonpathogenic

      MOLECULAR PLANT-MICROBE INTERACTIONS
    53. Rehli, M; Den Elzen, N; Cassady, AI; Ostrowski, MC; Hume, DA
      Cloning and characterization of the murine genes for bHLH-ZIP transcription factors TFEC and TFEB reveal a common gene organization for all MiT subfamily members

      GENOMICS
    54. Suske, G
      The Sp-family of transcription factors

      GENE
    55. de Jong, RN; van der Vliet, PC
      Mechanism of DNA replication in eukaryotic cells: cellular host factors stimulating adenovirus DNA replication

      GENE
    56. Zhang, SR; Varma, A; Williamson, PR
      The yeast Cryptococcus neoformans uses 'mammalian' enhancer sites in the regulation of the virulence gene, CNLAC1

      GENE
    57. Hettmann, C; Soldati, D
      Cloning and analysis of a Toxoplasma gondii histone acetyltransferase: A novel chromatin remodelling factor in Apicomplexan parasites

      NUCLEIC ACIDS RESEARCH
    58. Yankulov, K; Todorov, I; Romanowski, P; Licatalosi, D; Cilli, K; McCracken, S; Laskey, R; Bentley, DL
      MCM proteins are associated with RNA polymerase II holoenzyme

      MOLECULAR AND CELLULAR BIOLOGY
    59. Guzman, E; Lis, JT
      Transcription factor TFIIH is required for promoter melting in vivo

      MOLECULAR AND CELLULAR BIOLOGY
    60. Liu, L; Scolnick, DM; Trievel, RC; Zhang, HB; Marmorstein, R; Halazonetis, TD; Berger, SL
      p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage

      MOLECULAR AND CELLULAR BIOLOGY
    61. Eberharter, A; Sterner, DE; Schieltz, D; Hassan, A; Yates, JR; Berger, SL; Workman, JL
      The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae

      MOLECULAR AND CELLULAR BIOLOGY
    62. Sterner, DE; Grant, PA; Roberts, SM; Duggan, LJ; Belotserkovskaya, R; Pacella, LA; Winston, F; Workman, JL; Berger, SL
      Functional organization of the yeast SAGA complex: Distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction

      MOLECULAR AND CELLULAR BIOLOGY
    63. Li, Q; Imhof, A; Collingwood, TN; Urnov, FD; Wolffe, AP
      p300 stimulates transcription instigated by ligand-bound thyroid hormone receptor at a step subsequent to chromatin disruption

      EMBO JOURNAL
    64. Ohno, H; Kaneko, S; Lin, Y; Kobayashi, K; Murakami, S
      Human hepatitis B virus X protein augments the DNA binding of nuclear factor for IL-6 through its basic-leucine zipper domain

      JOURNAL OF MEDICAL VIROLOGY
    65. Cvekl, A; Kasanchi, F; Brady, JN; Piatigorsky, J
      Pax-6 interactions with TATA-box-binding protein and retinoblastoma protein

      INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
    66. Boyd, KE; Farnham, PJ
      Identification of target genes of oncogenic transcription factors

      PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE
    67. Liberati, C; di Silvio, A; Ottolenghi, S; Mantovani, R
      NF-Y binding to twin CCAAT boxes: Role of Q-rich domains and histone fold helices

      JOURNAL OF MOLECULAR BIOLOGY
    68. Angerer, ND; Du, Y; Nalbant, D; Williams, SC
      A short conserved motif is required for repressor domain function in the myeloid-specific transcription factor CCAAT enhancer-binding protein epsilon

      JOURNAL OF BIOLOGICAL CHEMISTRY
    69. Cook, T; Gebelein, B; Belal, M; Mesa, K; Urrutia, P
      Three conserved transcriptional repressor domains are a defining feature of the TIEG subfamily of Sp1-like zinc finger proteins

      JOURNAL OF BIOLOGICAL CHEMISTRY
    70. Ahlgren, R; Suske, G; Waterman, MR; Lund, J
      Role of Sp1 in cAMP-dependent transcriptional regulation of the bovine CYP11A gene

      JOURNAL OF BIOLOGICAL CHEMISTRY
    71. Hecht, A; Litterst, CM; Huber, O; Kemler, R
      Functional characterization of multiple transactivating elements in beta-catenin, some of which interact with the TATA-binding protein in vitro

      JOURNAL OF BIOLOGICAL CHEMISTRY
    72. Long, WP; Chen, X; Perdew, GH
      Protein kinase C modulates aryl hydrocarbon receptor nuclear translocator protein-mediated transactivation potential in a dimer context

      JOURNAL OF BIOLOGICAL CHEMISTRY
    73. Kwon, HS; Kim, MS; Edenberg, HJ; Hur, MW
      Sp3 and Sp4 can repress transcription by competing with Sp1 for the core cis-elements on the human ADH5/FDH minimal promoter

      JOURNAL OF BIOLOGICAL CHEMISTRY
    74. Jin, G; Howe, PH
      Transforming growth factor beta regulates clusterin gene expression via modulation of transcription factor c-Fos

      EUROPEAN JOURNAL OF BIOCHEMISTRY
    75. RIECHMANN JL; MEYEROWITZ EM
      THE AP2 EREBP FAMILY OF PLANT TRANSCRIPTION FACTORS/

      Biological chemistry
    76. NATARAJAN K; JACKSON BM; RHEE E; HINNEBUSCH AG
      YTAF(II)61 HAS A GENERAL ROLE IN RNA-POLYMERASE-II TRANSCRIPTION AND IS REQUIRED BY GCN4P TO RECRUIT THE SAGA COACTIVATOR COMPLEX

      MOLECULAR CELL
    77. GREGORY PD; SCHMID A; ZAVARI M; LUI L; BERGER SL; HORZ W
      ABSENCE OF GCN5 HAT ACTIVITY DEFINES A NOVEL STATE IN THE OPENING OF CHROMATIN AT THE PHO5 PROMOTER IN YEAST

      MOLECULAR CELL
    78. Grant, PA; Schieltz, D; Pray-Grant, MG; Yates, JR; Workman, JL
      The ATM-related cofactor Tra1 is a component of the purified SAGA complex

      MOLECULAR CELL
    79. DENISON C; KODADEK T
      SMALL-MOLECULE-BASED STRATEGIES FOR CONTROLLING GENE-EXPRESSION

      Chemistry & biology
    80. EBERHARTER A; JOHN S; GRANT PA; UTLEY RT; WORKMAN JL
      IDENTIFICATION AND ANALYSIS OF YEAST NUCLEOSOMAL HISTONE ACETYLTRANSFERASE COMPLEXES

      Methods (San Diego, Calif., Print)
    81. YAJIMA S; LEE SH; MINOWA T; MOURADIAN MM
      SP FAMILY TRANSCRIPTION FACTORS REGULATE EXPRESSION OF RAT D-2 DOPAMINE-RECEPTOR GENE

      DNA and cell biology
    82. SCHWECHHEIMER C; ZOURELIDOU M; BEVAN MW
      PLANT TRANSCRIPTION FACTOR STUDIES

      Annual review of plant physiology and plant molecular biology
    83. KODADEK T
      MECHANISTIC PARALLELS BETWEEN DNA-REPLICATION, RECOMBINATION AND TRANSCRIPTION

      Trends in biochemical sciences
    84. GRANT PA; STERNER DE; DUGGAN LJ; WORKMAN JL; BERGER SL
      THE SAGA UNFOLDS - CONVERGENCE OF TRANSCRIPTION REGULATORS IN CHROMATIN-MODIFYING COMPLEXES

      Trends in cell biology
    85. GREGORY PD; HORZ W
      LIFE WITH NUCLEOSOMES - CHROMATIN REMODELING IN GENE-REGULATION

      Current opinion in cell biology
    86. JOERS A; KRISTJUHAN A; KADAJA L; MAIMETS T
      TUMOR-ASSOCIATED MUTANTS OF P53 CAN INHIBIT TRANSCRIPTIONAL ACTIVITY OF P53 WITHOUT HETEROOLIGOMERIZATION

      Oncogene
    87. KRISTJUHAN A; JAKS V; RIMM I; TOOMING T; MAIMETS T
      OLIGOMERIZATION OF P53 IS NECESSARY TO INHIBIT ITS TRANSCRIPTIONAL TRANSACTIVATION PROPERTY AT HIGH-PROTEIN CONCENTRATION

      Oncogene
    88. HAVIV I; MATZA Y; SHAUL Y
      PX, THE HBV-ENCODED COACTIVATOR, SUPPRESSES THE PHENOTYPES OF TBP ANDTAF(II)250 MUTANTS

      Genes & development
    89. WANG L; LIU L; BERGER SL
      CRITICAL RESIDUES FOR HISTONE ACETYLATION BY GCN5, FUNCTIONING IN ADAAND SAGA COMPLEXES, ARE ALSO REQUIRED FOR TRANSCRIPTIONAL FUNCTION IN-VIVO

      Genes & development
    90. Bagby, S; Arrowsmith, CH; Ikura, M
      New perceptions of transcription factor properties from NMR

      BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
    91. ABDULKADIR SA; CASOLARO V; TAI AKF; THANOS D; ONO SJ
      HIGH-MOBILITY GROUP I Y PROTEIN FUNCTIONS AS A SPECIFIC COFACTOR FOR OCT-2A - MAPPING OF INTERACTION DOMAINS/

      Journal of leukocyte biology
    92. ROONEY RJ; ROTHAMMER K; FERNANDES ER
      MUTATIONAL ANALYSIS OF P50E4F SUGGESTS THAT DNA-BINDING ACTIVITY IS MEDIATED THROUGH AN ALTERNATIVE STRUCTURE IN A ZINC-FINGER DOMAIN THAT IS REGULATED BY PHOSPHORYLATION

      Nucleic acids research
    93. West, AG; Causier, BE; Davies, B; Sharrocks, AD
      DNA binding and dimerisation determinants of Antirrhinum majus MADS-box transcription factors

      NUCLEIC ACIDS RESEARCH
    94. ROESLER WJ; PARK EA
      HORMONE RESPONSE UNITS - ONE PLUS ONE EQUALS MORE THAN 2

      Molecular and cellular biochemistry
    95. CHERIYATH V; NOVINA CD; ROY AL
      TFII-I REGULATES V-BETA PROMOTER ACTIVITY THROUGH AN INITIATOR ELEMENT

      Molecular and cellular biology
    96. CHEN LF; ITO K; MURAKAMI Y; ITO Y
      THE CAPACITY OF POLYOMAVIRUS ENHANCER-BINDING PROTEIN 2-ALPHA-B (AML1CBFA2) TO STIMULATE POLYOMAVIRUS DNA-REPLICATION IS RELATED TO ITS AFFINITY FOR THE NUCLEAR MATRIX/

      Molecular and cellular biology
    97. BARLEV NA; POLTORATSKY V; OWENHUGHES T; YING C; LIU L; WORKMAN JL; BERGER SL
      REPRESSION OF GCN5 HISTONE ACETYLTRANSFERASE ACTIVITY VIA BROMODOMAIN-MEDIATED BINDING AND PHOSPHORYLATION BY THE KU-DNA-DEPENDENT PROTEIN-KINASE COMPLEX

      Molecular and cellular biology
    98. HAVIV I; SHAMAY M; DOITSH G; SHAUL Y
      HEPATITIS-B VIRUS PX TARGETS TFIIB IN TRANSCRIPTION COACTIVATION

      Molecular and cellular biology
    99. SAUTER P; MATTHIAS P
      COACTIVATOR OBF-1 MAKES SELECTIVE CONTACTS WITH BOTH THE POU-SPECIFICDOMAIN AND THE POU HOMEODOMAIN AND ACTS AS A MOLECULAR CLAMP ON DNA

      Molecular and cellular biology (Print)
    100. XU WT; EDMONDSON DG; ROTH SY
      MAMMALIAN GCN5 AND P CAF ACETYLTRANSFERASES HAVE HOMOLOGOUS AMINO-TERMINAL DOMAINS IMPORTANT FOR RECOGNITION OF NUCLEOSOMAL SUBSTRATES/

      Molecular and cellular biology


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Documento generato il 11/08/20 alle ore 16:04:54