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    1. Maki, N; Yamashita, O
      The 30kP protease A responsible for 30-kDa yolk protein degradation of thesilkworm, Bombyx mori: cDNA structure, developmental change and regulationby feeding

      INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
    2. Baba, H; Katsuzaki, H; Komiya, T; Yamashita, O; Imai, K
      Rapid degradation of the silkworm diapause hormone by trypsin and its suppression by VAP-map, a synthetic analog of the cuticular peptide of silkworm, Bm ACP-6.7 (VAP-Peptide)

      BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
    3. Yamashita, O
      Thermoelectric properties of heavily GaP- and P-doped Si0.95Ge0.05

      JOURNAL OF APPLIED PHYSICS
    4. Moribe, Y; Niimi, T; Yamashita, O; Yaginuma, T
      Samui, a novel cold-inducible gene, encoding a protein with a BAG domain similar to silencer of death domains (SODD/BAG-4), isolated from Bombyx diapause eggs

      EUROPEAN JOURNAL OF BIOCHEMISTRY
    5. Shiomi, K; Niimi, T; Imai, K; Yamashita, O
      Structure of the VAP-peptide (BmACP-6.7) gene in the silkworm, Bombyx moriand a possible regulation of its expression by BmFTZ-F1

      INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
    6. Yamashita, O; Sadatomi, N
      Thermoelectric properties of Si1-xGex(x <= 0.10) with alloy and dopant segregations

      JOURNAL OF APPLIED PHYSICS
    7. Saito, T; Yamashita, O; Miyata, T
      Critical review of endocrine disrupters in applied entomology.

      JAPANESE JOURNAL OF APPLIED ENTOMOLOGY AND ZOOLOGY
    8. Yoshida, H; Naito, O; Yamashita, O; Kitamura, S; Sakuma, T; Onose, Y; Nemoto, H; Hamano, T; Hatae, T; Nagashima, A; Matoba, T
      Multilaser and high spatially resolved multipoint Thomson scattering system for the JT-60U tokamak

      REVIEW OF SCIENTIFIC INSTRUMENTS
    9. Hatae, T; Nagashima, A; Kondoh, T; Kitamura, S; Kashiwabara, T; Yoshida, H; Naito, O; Shimizu, K; Yamashita, O; Sakuma, T
      YAG laser Thomson scattering diagnostic on the JT-60U

      REVIEW OF SCIENTIFIC INSTRUMENTS
    10. Yaginuma, T; Yamashita, O
      Oxygen consumption in relation to sorbitol utilization at the termination of diapause in eggs of the silkworm, Bombyx mori

      JOURNAL OF INSECT PHYSIOLOGY
    11. Makita, K; Yamashita, O
      Cold-rolled texture and magnetic properties of Fe-6.2%Si sintered material

      JOURNAL OF THE JAPAN INSTITUTE OF METALS
    12. Sadatomi, N; Yamashita, O
      Thermoelectric properties of Si-based materials

      JOURNAL OF THE JAPAN INSTITUTE OF METALS
    13. Makita, K; Yamashita, O
      Phase boundary structure in Nd-Fe-B sintered magnets

      APPLIED PHYSICS LETTERS
    14. Yamashita, O; Sadatomi, N
      Dependence of Seebeck coefficient on carrier concentration in heavily B- and P-doped Si1-xGex (x <= 0.05) system

      JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS
    15. SHIOMI K; NIIMI T; SATO Y; IMAI K; YAMASHITA O
      A HYDROPHOBIC PEPTIDE (VAP-PEPTIDE) OF THE SILKWORM, BOMBYX-MORI - A UNIQUE ROLE FOR ADULT ACTIVITY PROPOSED FROM GENE-EXPRESSION AND PRODUCTION AT THE TERMINAL PHASE OF METAMORPHOSIS

      Insect biochemistry and molecular biology
    16. SHIOMI K; SATO Y; IMAI K; YAMASHITA O
      A HYDROPHOBIC PEPTIDE (VAP-PEPTIDE) OF THE SILKWORM, BOMBYX-MORI - STRUCTURE, EXPRESSION AND AN ENHANCING FUNCTION OF DIAPAUSE HORMONE-ACTIVITY

      Insect biochemistry and molecular biology
    17. IMAI K; NOMURA T; KATSUZAKI H; KOMIYA T; YAMASHITA O
      MINIMUM STRUCTURE OF DIAPAUSE HORMONE REQUIRED FOR BIOLOGICAL-ACTIVITY

      Bioscience, biotechnology, and biochemistry
    18. YAMASHITA O
      MAGNETIC-PROPERTIES OF ND-FE-B MAGNETS PREPARED BY METAL INJECTION-MOLDING

      International journal of powder metallurgy
    19. YAMASHITA O; KISHIMOTO Y
      MAGNETIC-PROPERTIES OF ND-FE-B MAGNETS SINTERED USING GRANULATED POWDERS BY SPRAY-DRYING METHOD

      Powder Metallurgy
    20. ISHIDA S; FUJITA T; AKASAKA H; AKINO N; ANNOU K; AOYAGI T; ARAI T; ARAKAWA K; ASAKURA N; AZUMI M; BUDNY R; CHIBA S; DACOSTA O; EBISAWA N; FUJII T; FUKUDA T; FUNAHASHI A; GRISHAM L; GUNJI S; HAMAMATSU K; HASEGAWA Y; HATAE T; HIGASHIJIMA S; HIRATSUKA H; HIRAUCHI S; HIRAYAMA T; HONDA A; HONDA M; HOSOGANE N; ICHIGE H; IDE S; IKEDA Y; ISAKA M; ISAYAMA A; ISEI N; ISHII Y; ISOZAKI N; ITAMI K; ITOH T; IWAHASHI T; KAMADA Y; KAMINAGA A; KASHIWABARA T; KAWAI M; KAWAMATA Y; KAWANO Y; KAZAMA D; KAZAWA M; KIKUCHI M; KIMURA H; KIMURA T; KISHIMOTO H; KISHIMOTO Y; KITAMURA S; KIYONO K; KODAMA K; KOIDE Y; KOKUSEN S; KONDOH T; KONOSHIMA S; KOOG J; KRAMER GJ; KUBO H; KURIHARA K; KURITA G; KURIYAMA M; KUSAMA Y; MASAKI K; MATSUDA T; MATSUMOTO T; MATUKAWA M; MIURA T; MIYA N; MIYACHI K; MIYATA H; MIYO Y; MOGAKI K; MORI M; MORIMOTO M; MORIOKA S; MORIYAMA S; NAGAMI M; NAGASHIMA A; NAGASHIMA K; NAGAYA S; NAITO O; NAKAMURA Y; NEMOTO M; NEYATANI Y; NISHITANI T; OGIWARA N; OHGA T; OHSAWA M; OHSHIMA T; OIKAWA T; OKABE T; OKANO J; OMORI K; OMORI S; OMORI Y; ONOSE Y; OOHARA H; OZEKI T; SAIDOH M; SAIGUSA M; SAITO N; SAKASAI A; SAKATA S; SAKURAI S; SASAJIMA T; SATO M; SCOTT SD; SEIMIYA M; SEIKI H; SEKI M; SHIMADA M; SHIMIZU K; SHIMIZU M; SHIMONO M; SHINOZAKI S; SHIRAI H; SHITOMI M; SUGANUMA K; SUGIE T; SUNAOSHI H; TAKAHASHI M; TAKAHASHI S; TAKEJI S; TAKENAGA H; TAKIZUKA T; TAMAI H; TERAKADO M; TERAKADO T; TOBITA K; TOKUDA S; TOTSUKA T; TOYOKAWA Y; TOYOSHIMA N; TSUCHIYA K; TSUGITA T; TSUKAHARA Y; TUDA T; URAMOTO Y; USHIGUSA K; USUI K; YAGYU J; YAMAGIWA M; YAMAMOTO M; YAMAMOTO T; YAMASHITA O; YOKOKURA K; YOSHIDA H; YOSHIDA M; YOSHINO R
      ACHIEVEMENT OF HIGH FUSION PERFORMANCE IN JT-60U REVERSED SHEAR DISCHARGES (VOL 79, PG 3917, 1997)

      Physical review letters
    21. SATO T; MISAWA M; YAMASHITA O; YOSHIDA K; EBATA H; MARUYAMA K; IYETOMI H
      NETWORK STRUCTURE OF BORATE AND GERMANATE GLASSES STUDIED BY MEANS OFPULSED-NEUTRON SCATTERING

      Journal of non-crystalline solids
    22. SATO Y; SHIOMI K; SAITO H; IMAI K; YAMASHITA O
      PHE-X-PRO-ARG-LEU-NH2 PEPTIDE PRODUCING CELLS IN THE CENTRAL-NERVOUS-SYSTEM OF THE SILKWORM, BOMBYX-MORI

      Journal of insect physiology
    23. Katagiri, N; Ando, O; Yamashita, O
      Reduction of glycogen in eggs of the silkworm, Bombyx mori, by use of a trehalase inhibitor, trehazolin, and diapause induction in glycogen-reduced eggs

      JOURNAL OF INSECT PHYSIOLOGY
    24. Takahashi, M; Iwasaki, H; Niimi, T; Yamashita, O; Yaginuma, T
      Changing profiles of mRNA levels of Cdc2 and a novel Cdc2-related kinase (Bcdrk) in relation to ovarian development and embryonic diapause of Bombyx mori

      APPLIED ENTOMOLOGY AND ZOOLOGY
    25. MAKI N; YAMASHITA O
      PURIFICATION AND CHARACTERIZATION OF A PROTEASE DEGRADING 30 KDA YOLKPROTEINS OF THE SILKWORM, BOMBYX-MORI

      Insect biochemistry and molecular biology
    26. IWASAKI H; TAKAHASHI M; NIIMI T; YAMASHITA O; YAGINUMA T
      CLONING OF CDNAS ENCODING BOMBYX HOMOLOGS OF CDC2 AND CDC2-RELATED KINASE FROM EGGS

      Insect molecular biology
    27. NIIMI T; MORITA S; YAMASHITA O; YAGINUMA T
      THE PROFILES OF MESSENGER-RNA LEVELS FOR BHR39, A BOMBYX HOMOLOG OF DROSOPHILA HORMONE-RECEPTOR-39, AND BOMBYX FTZ-F1 IN THE COURSE OF EMBRYONIC-DEVELOPMENT AND DIAPAUSE

      Development, genes and evolution
    28. HATAE T; NAGASHIMA A; YOSHIDA H; NAITO O; KITAMURA S; YAMASHITA O; KAZAMA D; ONOSE Y; MATOBA T
      FIRST OPERATION RESULTS OF YAG LASER THOMSON SCATTERING SYSTEM ON JT-60U

      Fusion engineering and design
    29. YOSHIDA H; NAITO O; MATOBA T; YAMASHITA O; KITAMURA S; HATAE T; NAGASHIMA A
      QUANTITATIVE METHOD FOR PRECISE, QUICK, AND RELIABLE ALIGNMENT OF COLLECTION OBJECT FIELDS IN THE JT-60U THOMSON SCATTERING DIAGNOSTIC

      Review of scientific instruments
    30. ISHIDA S; FUJITA T; AKASAKA H; AKINO N; ANNOU K; AOYAGI T; ARAI T; ARAKAWA K; ASAKURA N; AZUMI M; BUDNY R; CHIBA S; DACOSTA O; EBISAWA N; FUJII T; FUKUDA T; FUNAHASHI A; GRISHAM L; GUNJI S; HAMAMATSU K; HASEGAWA Y; HATAE T; HIGASHIJIMA S; HIRATSUKA H; HIRAUCHI S; HIRAYAMA T; HONDA A; HONDA M; HOSOGANE N; ICHIGE H; IDE S; IKEDA Y; ISAKA M; ISAYAMA A; ISEI N; ISHII Y; ISOZAKI N; ITAMI K; ITOH T; IWAHASHI T; KAMADA Y; KAMINAGA A; KASHIWABARA T; KAWAI M; KAWAMATA Y; KAWANO Y; KAZAMA D; KAZAWA M; KIKUCHI M; KIMURA H; KIMURA T; KISHIMOTO H; KISHIMOTO Y; KITAMURA S; KIYONO K; KODAMA K; KOIDE Y; KOKUSEN S; KONDOH T; KONOSHIMA S; KOOG J; KRAMER GJ; KUBO H; KURIHARA K; KURITA G; KURIYAMA M; KUSAMA Y; MASAKI K; MATSUDA T; MATSUMOTO T; MATUKAWA M; MIURA T; MIYA N; MIYACHI K; MIYATA H; MIYO Y; MOGAKI K; MORI M; MORIMOTO M; MORIOKA S; MORIYAMA S; NAGAMI M; NAGASHIMA A; NAGASHIMA K; NAGAYA S; NAITO O; NAKAMURA Y; NEMOTO M; NEYATANI Y; NISHITANI T; OGIWARA N; OHGA T; OHSAWA M; OHSHIMA T; OIKAWA T; OKABE T; OKANO J; OMORI K; OMORI S; OMORI Y; ONOSE Y; OOHARA H; OZEKI T; SAIDOH M; SAIGUSA M; SAITO N; SAKASAI A; SAKATA S; SAKURAI S; SASAJIMA T; SATO M; SCOTT SD; SEIMIYA M; SEIKI H; SEKI M; SHIMADA M; SHIMIZU K; SHIMIZU M; SHIMONO M; SHINOZAKI S; SHIRAI H; SHITOMI M; SUGANUMA K; SUGIE T; SUNAOSHI H; TAKAHASHI M; TAKAHASHI S; TAKEJI S; TAKENAGA H; TAKIZUKA T; TAMAI H; TERAKADO M; TERAKADO T; TOBITA K; TOKUDA S; TOTSUKA T; TOYOKAWA Y; TOYOSHIMA N; TSUCHIYA K; TSUGITA T; TSUKAHARA Y; TUDA T; URAMOTO Y; USHIGUSA K; USUI K; YAGYU J; YAMAGIWA M; YAMAMOTO M; YAMAMOTO T; YAMASHITA O; YOKOKURA K; YOSHIDA H; YOSHIDA M; YOSHINO R
      ACHIEVEMENT OF HIGH FUSION PERFORMANCE IN JT-60U REVERSED SHEAR DISCHARGES

      Physical review letters
    31. STARNECKER G; KOCH PB; MATSUMOTO S; ENDO K; YAMASHITA O
      OCCURRENCE OF 4 NEUROPEPTIDES IN THE ANTERIOR CENTRAL-NERVOUS-SYSTEM OF THE BUTTERFLY PRECIS-COENIA

      Naturwissenschaften
    32. NAKAMURA Y; KITADA T; KUSHIMA T; KUDO T; NISHIMURA J; TATSUTA N; YAMASHITA O
      INCLUSION CRYSTALS FROM NAPHTHALENOPHANES IN AROMATIC SOLVENTS

      Molecular crystals and liquid crystals science and technology. Section A, Molecular crystals and liquid crystals
    33. XU WH; STAO Y; YAMASHITA O
      MOLECULAR-CLONING OF PHEROMONE BIOSYNTHESIS ACTIVATING NEUROPEPTIDE IN SILKWORM, BOMBYX-MORI

      SCIENCE IN CHINA SERIES C-LIFE SCIENCES
    34. TAKAHASHI M; NIIMI T; ICHIMURA H; SASAKI T; YAMASHITA O; YAGINUMA T
      CLONING OF A B-TYPE CYCLIN HOMOLOG FROM BOMBYX-MORI AND THE PROFILES OF ITS MESSENGER-RNA LEVEL IN NONDIAPAUSE AND DIAPAUSE EGGS

      Development, genes and evolution
    35. IMAI K; SUGIURA K; KOMIYA T; YAMASHITA O
      ISOLATION AND PARTIAL STRUCTURE OF A UNIQUE LIPOPHILIC PEPTIDE, VAP PEPTIDE, FROM THE HEADS OF MALE SILKWORM MOTHS

      Bioscience, biotechnology, and biochemistry
    36. YAGINUMA T; MIZUNO T; MIZUNO C; IKEDA M; WADA T; HATTORI K; YAMASHITA O; HAPP GM
      TREHALASE IN THE SPERMATOPHORE FROM THE BEAN SHAPED ACCESSORY-GLAND OF THE MALE MEALWORM BEETLE, TENEBRIO-MOLITOR - PURIFICATION, KINETIC-PROPERTIES AND LOCALIZATION OF THE ENZYME

      Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
    37. YAMASHITA O
      DIAPAUSE HORMONE OF THE SILKWORM, BOMBYX-MORI - STRUCTURE, GENE-EXPRESSION AND FUNCTION

      Journal of insect physiology
    38. YAMANE T; KANI T; HATANAKA T; SUZUKI A; ASHIDA T; KOBAYASHI T; ITO S; YAMASHITA O
      STRUCTURE OF A NEW ALKALINE SERINE-PROTEASE (M-PROTEASE) FROM BACILLUS SP KSM-K16

      Acta crystallographica. Section D, Biological crystallography
    39. ITOH Y; YAMASHITA O; HACHIMORI A; KOJIMA M; SUZUKI S
      PHOTOCHEMISTRY OF AMPHIPHILIC COPOLYMERS - FLUORESCENCE-SPECTRA OF COPOLYMERS OF 1-(2-NAPHTHYL)ETHYL METHACRYLATE AND SODIUM 2-ACRYLAMIDO-2-METHYLPROPANESULFONATE IN SOLUTION

      Journal of polymer science. Part A, Polymer chemistry
    40. ABERNATHY RL; NACHMAN RJ; TEAL PEA; YAMASHITA O; TUMLINSON JH
      PHEROMONOTROPIC ACTIVITY OF NATURALLY-OCCURRING PYROKININ INSECT NEUROPEPTIDES (FXPRLAMIDE) IN HELICOVERPA-ZEA

      Peptides
    41. XU WH; SATO Y; IKEDA M; YAMASHITA O
      MOLECULAR CHARACTERIZATION OF THE GENE ENCODING THE PRECURSOR PROTEINOF DIAPAUSE HORMONE AND PHEROMONE BIOSYNTHESIS ACTIVATING NEUROPEPTIDE (DH-PBAN) OF THE SILKWORM, BOMBYX-MORI AND ITS DISTRIBUTION IN SOME INSECTS

      Biochimica et biophysica acta, N. Gene structure and expression
    42. YOSHIDA H; NAITO O; YAMASHITA O; KITAMURA S; NAGASHIMA A; MATOBA T
      BEAM COMBINER FOR TRANSIENT PHENOMENA MEASUREMENT IN THE JT-60 THOMSON SCATTERING DIAGNOSTIC

      Review of scientific instruments
    43. XU WH; SATO Y; IKEDA M; YAMASHITA O
      STAGE-DEPENDENT AND TEMPERATURE-CONTROLLED EXPRESSION OF THE GENE ENCODING THE PRECURSOR PROTEIN OF DIAPAUSE HORMONE AND PHEROMONE BIOSYNTHESIS ACTIVATING NEUROPEPTIDE IN THE SILKWORM, BOMBYX-MORI

      The Journal of biological chemistry
    44. PINYARAT W; SHIMADA T; XU WH; SATO Y; YAMASHITA O; KOBAYASHI M
      LINKAGE ANALYSIS OF THE GENE ENCODING PRECURSOR PROTEIN OF DIAPAUSE HORMONE AND PHEROMONE BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE IN THE SILKMOTH, BOMBYX-MORI

      Genetical Research
    45. SUWAN S; ISOBE M; YAMASHITA O; MINAKATA H; IMAI K
      SILKWORM DIAPAUSE HORMONE, STRUCTURE-ACTIVITY-RELATIONSHIPS INDISPENSABLE ROLE OF C-TERMINAL AMIDE

      Insect biochemistry and molecular biology
    46. KADONOOKUDA K; AMORNSAK W; YAMASHITA O
      CONTROLLED ECDYSTEROID ACCUMULATION IN EGGS OF THE SILKWORM, BOMBYX-MORI, BY AN IMIDAZOLE COMPOUND (KK-42), AND EMBRYOGENESIS IN THESE EGGS

      Archives of insect biochemistry and physiology
    47. SAITO H; TAKEUCHI Y; TAKEDA R; HAYASHI Y; WATANABE K; SHIN M; IMAI K; ISOBE M; YAMASHITA O
      THE CORE AND COMPLEMENTARY SEQUENCE RESPONSIBLE FOR BIOLOGICAL-ACTIVITY OF THE DIAPAUSE HORMONE OF THE SILKWORM, BOMBYX-MORI

      Peptides
    48. SU ZH; IKEDA M; SATO Y; SAITO H; IMAI K; ISOBE M; YAMASHITA O
      MOLECULAR CHARACTERIZATION OF OVARY TREHALASE OF THE SILKWORM, BOMBYX-MORI AND ITS TRANSCRIPTIONAL ACTIVATION BY DIAPAUSE HORMONE

      Biochimica et biophysica acta, N. Gene structure and expression
    49. TANAKA S; TATSUTA N; YAMASHITA O; ISOBE M
      RING-OPENING OF ALKYNYL SUGARS BY NICHOLAS REACTION - APPLICATION TO ENANTIOSELECTIVE SYNTHESIS OF OXEPANE SUBUNITS OF MARINE TRANS-FUSED POLYETHER TOXINS

      Tetrahedron
    50. SHIOMI K; ISHIDA Y; IKEDA M; SATO Y; SAITO H; IMAI K; ISOBE M; YAMASHITA O
      INDUCTION OF NONDIAPAUSE EGGS BY INJECTION OF ANTI-DIAPAUSE HORMONE RABBIT SERUM INTO THE DIAPAUSE TYPE OF THE SILKWORM, BOMBYX-MORI

      Journal of insect physiology
    51. SATO Y; IKEDA M; YAMASHITA O
      NEUROSECRETORY-CELLS EXPRESSING THE GENE FOR COMMON PRECURSOR FOR DIAPAUSE HORMONE AND PHEROMONE BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE IN THE SUBESOPHAGEAL GANGLION OF THE SILKWORM, BOMBYX-MORI

      General and comparative endocrinology
    52. NIIMI T; YAMASHITA O; YAGINUMA T
      DEVELOPMENTAL PROFILE OF THE GENE-EXPRESSION OF A BOMBYX HOMOLOG OF MAMMALIAN SORBITOL DEHYDROGENASE DURING EMBRYOGENESIS IN NONDIAPAUSE EGGS

      Comparative biochemistry and physiology. B. Comparative biochemistry
    53. NACHMAN RJ; HOLMAN GM; SCHOOFS L; YAMASHITA O
      SILKWORM DIAPAUSE INDUCTION ACTIVITY OF MYOTROPIC PYROKININ (FXPRLAMIDE) INSECT NEUROPEPTIDES

      Peptides
    54. SATO Y; OGUCHI M; MENJO N; IMAI K; SAITO H; IKEDA M; ISOBE M; YAMASHITA O
      PRECURSOR POLYPROTEIN FOR MULTIPLE NEUROPEPTIDES SECRETED FROM THE SUBESOPHAGEAL GANGLION OF THE SILKWORM BOMBYX-MORI - CHARACTERIZATION OFTHE CDNA-ENCODING THE DIAPAUSE HORMONE PRECURSOR AND IDENTIFICATION OF ADDITIONAL PEPTIDES

      Proceedings of the National Academy of Sciences of the United Statesof America
    55. IKEDA M; SU ZH; SAITO H; IMAI K; SATO Y; ISOBE M; YAMASHITA O
      INDUCTION OF EMBRYONIC DIAPAUSE AND STIMULATION OF OVARY TREHALASE ACTIVITY IN THE SILKWORM, BOMBYX-MORI, BY SYNTHETIC DIAPAUSE HORMONE

      Journal of insect physiology
    56. NIIMI T; YAMASHITA O; YAGINUMA T
      A COLD-INDUCIBLE BOMBYX GENE ENCODING A PROTEIN SIMILAR TO MAMMALIAN SORBITOL DEHYDROGENASE - YOLK NUCLEI-DEPENDENT GENE-EXPRESSION IN DIAPAUSE EGGS

      European journal of biochemistry
    57. SU ZH; SATO Y; YAMASHITA O
      PURIFICATION, CDNA CLONING AND NORTHERN BLOT ANALYSIS OF TREHALASE OFPUPAL MIDGUT OF THE SILKWORM, BOMBYX-MORI

      Biochimica et biophysica acta


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Documento generato il 16/01/21 alle ore 18:54:28