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Titolo:
ECTOINE FUNCTIONS AS AN OSMOPROTECTANT IN BACILLUS-SUBTILIS AND IS ACCUMULATED VIA THE ABC-TRANSPORT SYSTEM OPUC
Autore:
JEBBAR M; VONBLOHN C; BREMER E;
Indirizzi:
MAX PLANCK INST TERR MIKROBIOL,KARL VON FRISCH STR D-35043 MARBURG GERMANY MAX PLANCK INST TERR MIKROBIOL D-35043 MARBURG GERMANY UNIV MARBURG,FACHBEREICH BIOL,MIKROBIOL LAB D-35032 MARBURG GERMANY
Titolo Testata:
FEMS microbiology letters
fascicolo: 2, volume: 154, anno: 1997,
pagine: 325 - 330
SICI:
0378-1097(1997)154:2<325:EFAAOI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCINE BETAINE; ESCHERICHIA-COLI; OSMOREGULATION; OSMOADAPTATION; PROU;
Keywords:
BACILLUS SUBTILIS; ABC-TRANSPORTER; OSMOPROTECTANT; ECTOINE; GLYCINE BETAINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M. Jebbar et al., "ECTOINE FUNCTIONS AS AN OSMOPROTECTANT IN BACILLUS-SUBTILIS AND IS ACCUMULATED VIA THE ABC-TRANSPORT SYSTEM OPUC", FEMS microbiology letters, 154(2), 1997, pp. 325-330

Abstract

We report here that the cyclic amino acid ectoine functions as an osmoprotectant for the soil bacterium Bacillus subtilis. Growth experiments with a set of B, subtilis strains that carry defined mutations in the glycine betaine transport systems OpuA, OpuC and OpuD and the choline transport system OpuB revealed that ectoine was specifically accumulated via the ABC-transport system OpuC. Competition experiments employing unlabeled ectoine and radiolabeled glycine betaine showed that the OpuC transport system has a low affinity for ectoine with a K-i value of approximately 1.5 mM, Ectoine was identified by H-1 NMR spectroscopy in the solute pool of cells grown in the presence of ectoine. Ectoine could not be used by B, subtilis as sole carbon or nitrogen source, Our data thus characterise ectoine as a metabolically inert stress compound for B, subtilis and establish a crucial role for the ABC-transport system OpuC for the acquisition of the osmoprotectant ectoine from the environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 10:05:28