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Titolo:
ACCUMULATION OF CLUSTERIN SULFATED GLYCOPROTEIN-2 IN DEGENERATING PACHYTENE SPERMATOCYTES OF ADULT RATS TREATED WITH METHOXYACETIC ACID
Autore:
CLARK AM; MAGUIRE SM; GRISWOLD MD;
Indirizzi:
WASHINGTON STATE UNIV,DEPT BIOCHEM & BIOPHYS PULLMAN WA 99164
Titolo Testata:
Biology of reproduction
fascicolo: 4, volume: 57, anno: 1997,
pagine: 837 - 846
SICI:
0006-3363(1997)57:4<837:AOCSGI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; MESSENGER-RNA; SERTOLI CELLS; MOLECULAR-CLONING; GENE-EXPRESSION; APOLIPOPROTEIN-J/CLUSTERIN; SEMINIFEROUS TUBULES; PROTEIN SECRETION; GERM-CELLS; TESTIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Clark et al., "ACCUMULATION OF CLUSTERIN SULFATED GLYCOPROTEIN-2 IN DEGENERATING PACHYTENE SPERMATOCYTES OF ADULT RATS TREATED WITH METHOXYACETIC ACID", Biology of reproduction, 57(4), 1997, pp. 837-846

Abstract

Clusterin is a ubiquitous glycoprotein that is produced constitutively by Sertoli cells at relatively high amounts. Its association with apoptosis, damage, disease, and repair in nongonadal tissues led us to investigate whether clusterin could be part of a damage-induced response in Sertoli cells brought on by apoptosis of an adjacent cell type. Therefore, the objective of this study was to treat adult rats with methoxyacetic acid (MAA) to selectively destroy pachytene spermatocytes, examine the localization and expression of testicular clusterin, and relate this to the timing of DNA fragmentation, a hallmark of apoptosis. Clusterin protein was localized to the cytoplasm of pachytene spermatocytes at 6 h post-MAA, whereas clusterin mRNA was localized to Sertoli cells. Morphological degeneration of dying cells and DNA fragmentation were not seen until 12 h. Thus, Sertoli cell-derived clusterin hadaccumulated in the cytoplasm of degenerating spermatocytes early in the apoptotic process. On the basis of these results and the known binding of clusterin to hydrophobic macromolecules, we hypothesize that clusterin is produced by Sertoli cells as a mechanism to ''clear'' potentially harmful cellular components during the degeneration of germ cells and remodeling of their membranes that occur normally during spermatogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:31:37