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Titolo:
SPONTANEOUS ACTIVATION OF OVULATED MOUSE EGGS - TIME-DEPENDENT EFFECTS ON M-PHASE EXIT, CORTICAL GRANULE EXOCYTOSIS, MATERNAL MESSENGER-RIBONUCLEIC-ACID RECRUITMENT, AND INOSITOL 1,4,5-TRISPHOSPHATE SENSITIVITY
Autore:
XU Z; ABBOTT A; KOPF GS; SCHULTZ RM; DUCIBELLA T;
Indirizzi:
TUFTS UNIV,SCH MED,DEPT OBSTET & GYNECOL,136 HARRISON AVE BOSTON MA 02111 TUFTS UNIV,SCH MED,DEPT OBSTET & GYNECOL BOSTON MA 02111 TUFTS UNIV,SCH MED,DEPT ANAT & CELLULAR BIOL BOSTON MA 02111 TUFTS UNIV NEW ENGLAND MED CTR BOSTON MA 02111 UNIV PENN,CTR RES REPROD & WOMENS HLTH PHILADELPHIA PA 19104 UNIV PENN,DEPT BIOL PHILADELPHIA PA 19104
Titolo Testata:
Biology of reproduction
fascicolo: 4, volume: 57, anno: 1997,
pagine: 743 - 750
SICI:
0006-3363(1997)57:4<743:SAOOME>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACYTOPLASMIC SPERM INJECTION; CUMULUS-CORONAL MORPHOLOGY; METAPHASE-II ARREST; PROTEIN-KINASE-II; MEIOTIC MATURATION; ZONA-PELLUCIDA; HUMAN OOCYTES; CELL-CYCLE; IN-VITRO; MAMMALIAN OOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
Z. Xu et al., "SPONTANEOUS ACTIVATION OF OVULATED MOUSE EGGS - TIME-DEPENDENT EFFECTS ON M-PHASE EXIT, CORTICAL GRANULE EXOCYTOSIS, MATERNAL MESSENGER-RIBONUCLEIC-ACID RECRUITMENT, AND INOSITOL 1,4,5-TRISPHOSPHATE SENSITIVITY", Biology of reproduction, 57(4), 1997, pp. 743-750

Abstract

With increasing time after ovulation, mammalian eggs become more sensitive to agonists of activation in vitro or may undergo spontaneous activation in vivo. We have tested the hypothesis that postovulatory eggs undergo time-dependent cell cycle and cytoplasmic changes that result in a partially activated state, accounting for their time-dependent susceptibility to activate. In vivo changes in key activation markers in mouse eggs were quantified at 13, 16, and 22 h post-hCG (1, 4, and 10 h postovulation). Spontaneous activation was first detected at 16 h, with a 20-25% decrease in the activities of histone H1 and mitogen-activated protein (MAP) kinases and with 3% of eggs undergoing both anaphase onset and a partial loss of cortical granules. By 22 h, more than 60% of eggs were in anaphase, H1 and MAP kinase activities had decreased 40-50%, the extent of zona pellucida modification had increased, and proteins normally synthesized after fertilization had appeared. Pronuclear formation in response to inositol 1,4,5-trisphosphate injection increased dramatically from 10% at 13 h to about 40% and 90% at 16 h and 22 h, respectively. The partial decreases (less than those afterfertilization) in H1 and MAP kinase activities provide a likely biochemical basis for the increased sensitivity of eggs to agonists, seen over time, that results in pronuclear formation. Also, all of these time-dependent changes caution against the use of mouse eggs > 16 h afterhCG administration in studying the mechanism of normal fertilization and have implications for animal and human in vitro fertilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:03:37