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Titolo:
THE RATIO OF PROGESTERONE-RECEPTOR ISOFORMS CHANGES IN THE MONKEY CORPUS-LUTEUM DURING THE LUTEAL-PHASE OF THE MENSTRUAL-CYCLE
Autore:
DUFFY DM; WELLS TR; HALUSKA GJ; STOUFFER RL;
Indirizzi:
OREGON REG PRIMATE RES CTR,DIV REPROD SCI,505 NW 185TH AVE BEAVERTON OR 97006 OREGON REG PRIMATE RES CTR,DIV REPROD SCI BEAVERTON OR 97006
Titolo Testata:
Biology of reproduction
fascicolo: 4, volume: 57, anno: 1997,
pagine: 693 - 699
SICI:
0006-3363(1997)57:4<693:TROPIC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; HUMAN-BREAST-CANCER; 3-BETA-HYDROXYSTEROID DEHYDROGENASE INHIBITOR; RHESUS-MONKEYS; GRANULOSA-CELLS; B EXPRESSION; A FORM; ESTROGEN; LOCALIZATION; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
D.M. Duffy et al., "THE RATIO OF PROGESTERONE-RECEPTOR ISOFORMS CHANGES IN THE MONKEY CORPUS-LUTEUM DURING THE LUTEAL-PHASE OF THE MENSTRUAL-CYCLE", Biology of reproduction, 57(4), 1997, pp. 693-699

Abstract

Recent evidence suggests that progesterone is required for ovulation,luteinization, and the maintenance of luteal structure and function in primates. Progesterone action is mediated by intracellular progesterone receptors (PRs), and PRs are detectable by immunocytochemistry in the monkey corpus luteum. However, changes in total luteal PR and PR isoform expression have not been quantitated in the corpus luteum during its life span in the menstrual cycle. This study was initiated to identify and quantify PR isoforms in the macaque corpus luteum throughout the luteal phase of the natural cycle by means of Western blotting. Several antibodies generated against the human PR recognized two bandsof consistent molecular weights in monkey tissues, and these bands comigrated with PR-A and PR-B from human T47D cells. Taken together, these data suggest that the two proteins identified were macaque PR-A andPR-B. The estimated molecular weights of monkey PR-A and PR-B were approximately 90 000 and 120 000, respectively. PRs were detected in a variety of macaque tissues, including the endometrium, whole ovary, anddecidua, but not in spleen, which is PR-negative by other techniques. Whereas PR-A was the predominant isoform observed in endometrium and decidua, PR-B predominated in the ovary without a dominant follicle orcorpus luteum as well as in the corpus luteum. In luteal tissue, PR-Alevels decreased (p < 0.05) over the course of the luteal phase, while PR-B levels were unchanged. Hence the ratio of PR-B to PR-A (PR-B:PR-A) increased (p < 0.05) from early to very late luteal phase. Since PR-B:PR-A can alter gene expression in response to progestins and antiprogestins in vitro, the temporal changes in PR-B:PR-A in the monkey corpus luteum may contribute to functional differences in luteal responses to progesterone and other steroids in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 13:20:16