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Titolo:
ANALYSIS OF CLOZAPINE AND NORCLOZAPINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
AKERMAN KK;
Indirizzi:
KUOPIO UNIV HOSP,DEPT CLIN CHEM,POB 1777 SF-70211 KUOPIO FINLAND UNIV KUOPIO SF-70211 KUOPIO FINLAND
Titolo Testata:
Journal of chromatography B. Biomedical sciences and applications
fascicolo: 2, volume: 696, anno: 1997,
pagine: 253 - 259
SICI:
0378-4347(1997)696:2<253:AOCANB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; SCHIZOPHRENIC-PATIENTS; HUMAN SERUM; N-DESMETHYLCLOZAPINE; CLINICAL-RESPONSE; MAJOR METABOLITES; PLASMA-LEVELS; PHARMACOKINETICS;
Keywords:
CLOZAPINE; NORCLOZAPINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
K.K. Akerman, "ANALYSIS OF CLOZAPINE AND NORCLOZAPINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Journal of chromatography B. Biomedical sciences and applications, 696(2), 1997, pp. 253-259

Abstract

A simple and reliable method for analyzing the concentrations of clozapine and its biologically active metabolite, norclozapine, in human serum or plasma has been developed. This method is based on reversed-phase high-performance liquid chromatography (HPLC) with automated solid-phase extraction (SPE). For HPLC analysis, samples and standards are prepared with an ASPEC automatic sample preparator using 100 mg Bond-Elut C-18 SPE columns. The HPLC assay is an isocratic method with a mobile phase of acetonitrile-methanol-10 mM dipotassium hydrogenphosphate, pH 3.7 (30:2:100, v/v/v) at a flow-rate of 1.5 ml/min with a C-8 reversed-phase column. Detection is performed with a diode array detectorset at 220 nm and with peak purity analyses at 210-365 nm. The absolute recovery varied from 85 and 95%. The intra-assay coefficients of variation (C.V.s) were from 4.2 to 8.0% and the inter-assay C.V.s were from 1.1 to 9.3% at therapeutic drug concentrations. The detection limit is 15 nmol/l. The method has been developed for use in a clinical laboratory for therapeutic drug monitoring. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 06:04:42