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Titolo:
PERIPANCREATIC FLUID CYTOLOGY - DETECTION OF EARLY REJECTION VERSUS GRAFT PANCREATITIS AFTER CANINE PANCREATIC TRANSPLANTATION
Autore:
SUZUKI Y; KURODA Y; TANIOKA Y; MATSUMOTO S; KIM Y; KU Y; TSUKAMOTO R; SAITOH Y;
Indirizzi:
KOBE UNIV,SCH MED,DEPT SURG 1,CHUO KU,7-5-2 KUSUNOKI CHO KOBE HYOGO 650 JAPAN KOBE UNIV,SCH MED,DEPT CLIN PATHOL & CYTOL,CHUO KU KOBE HYOGO 650 JAPAN
Titolo Testata:
World journal of surgery
fascicolo: 8, volume: 21, anno: 1997,
pagine: 880 - 885
SICI:
0364-2313(1997)21:8<880:PFC-DO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERCUTANEOUS BIOPSY; EXOCRINE DIVERSION; ALLOGRAFTS; DIAGNOSIS; JUICE; CT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
Y. Suzuki et al., "PERIPANCREATIC FLUID CYTOLOGY - DETECTION OF EARLY REJECTION VERSUS GRAFT PANCREATITIS AFTER CANINE PANCREATIC TRANSPLANTATION", World journal of surgery, 21(8), 1997, pp. 880-885

Abstract

It is particularly difficult to distinguish between early rejection and graft pancreatitis when early rejection produces an elevated serum amylase level. In this study we determined whether peripancreatic fluid cytology (PPC) can differentiate early acute rejection and graft pancreatitis as an alternative diagnostic tool to graft biopsy that has the potential of pancreatic fistula and hemorrhage. Sixty-two dogs received either a segmental pancreas allograft (n = 25) or autograft (n = 37) heterotopically in the neck This study included five groups: allografts without immunosuppression (group A, n = 12), allografts with immuno/suppression (group B, n = 13), autografts without immunosuppression (group C, n = 11), autografts with immunosuppression (group D, n = 12), and autografts treated by 45 minutes of pretransplant warm ischemia to induce acute graft pancreatitis (group E, n = 14). A closed suction drainage catheter was placed next to the graft to collect peripancreatic fluid daily after the transplant. PFC was performed using May-Gruenwald-Giemsa double-staining technique and compared to the corresponding histology through the observation period. In analyses of 50 functioning grafts, PFC performed on day I showed similar neutrophil accumulations in all groups. In sharp contrast, on days 3 and 6, group A haddramatically increased mononuclear cell concentrations in PPC, whereas groups Il, C, and D showed significantly Lower concentrations, the percent of mononuclear cells among total leukocytes being 47.3 +/- 23.4%, 11.8 +/- 4.9%, 4.3 +/- 1.8%, and 6.4 +/- 2.4% (day 3); and 32.7 +/-9.8%, 10.5 +/- 4.8%, 7.2 +/- 4.2%, and 8.6 +/- 6.4% (day 6) in groupsA, B, C, and D, respectively. On the other hand. in group E numerous degenerating neutrophils with a marked to moderate increase in necrotic tissue fragments were observed by PPC on days 3 and 6. In terms of graft histology on days 3 and 6, group A showed interstitial mononuclear cell infiltration indicating an acute rejection process, whereas groups B, C, and D had minimal inflammatory cell infiltration. In group Egraft pancreatitis was histologically confirmed on days 3 and 6. These results suggest that PFC after pancreas transplantation could be a safe, simple, useful diagnostic tool for discriminating early graft rejection from graft pancreatitis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 23:29:01