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Titolo:
QUANTITATION AND HISTOCHEMICAL-LOCALIZATION OF GALECTIN-1 AND GALECTIN-1-REACTIVE GLYCOCONJUGATES IN FETAL DEVELOPMENT OF BOVINE ORGANS
Autore:
KALTNER H; LIPS KS; REUTER G; LIPPERT S; SINOWATZ F; GABIUS HJ;
Indirizzi:
UNIV MUNICH,TIERARZLICHE FAK,INST PHYSIOL CHEM,VET STR 13 D-80539 MUNICH GERMANY UNIV MUNICH,TIERARZLICHE FAK,INST PHYSIOL CHEM D-80539 MUNICH GERMANY UNIV MUNICH,TIERARZLICHE FAK,INST TIERANAT D-80539 MUNICH GERMANY
Titolo Testata:
Histology and histopathology
fascicolo: 4, volume: 12, anno: 1997,
pagine: 945 - 960
SICI:
0213-3911(1997)12:4<945:QAHOGA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSIDE-BINDING LECTIN; ANIMAL LECTINS; HEART-MUSCLE; MOUSE EMBRYOGENESIS; VERTEBRATE LECTINS; LARGE FAMILY; CELL; EXPRESSION; PROTEINS; TISSUES;
Keywords:
LECTIN; GALECTIN; GLYCOPROTEINS; GLYCOLIPIDS; DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
66
Recensione:
Indirizzi per estratti:
Citazione:
H. Kaltner et al., "QUANTITATION AND HISTOCHEMICAL-LOCALIZATION OF GALECTIN-1 AND GALECTIN-1-REACTIVE GLYCOCONJUGATES IN FETAL DEVELOPMENT OF BOVINE ORGANS", Histology and histopathology, 12(4), 1997, pp. 945-960

Abstract

The display of cellular oligosaccharide chains is known to undergo marked developmental changes, as monitored histochemically with plant lectins. In conjunction with endogenous lectins respective ligand structures may have a functional role during fetal development. The assumption of a recognitive, functionally productive interplay prompts the study of the expression of a tissue lectin and of lectin-reactive glycoconjugates concomitantly. Focusing on common beta-galactosides as constituents of oligosaccharide chains and the predominant member of the family of galectins in mammals, namely galectin-1, the question thereforeis addressed as to whether expression of lectin and lectin-reactive glycoconjugates exhibits alterations, assessed in three morphologicallydefined fetal stages and in adult bovine organs. Using a sandwich ELISA, the level of the rather ubiquitous galectin-1 is mostly increased in adult organs relative to respective fetal stages, except for the case of kidney. This developmental course is seen rather seldom, when the amounts of lectin-reactive glycoproteins or glycolipids are quantitated in solid-phase assays after tissue homogenization. Western blotting, combined with probing by labeled galectin-1, discloses primarily quantitative changes in the reactivity of individual glycoproteins. Performing the same assays on extract aliquots with a plant agglutinin, namely the galactoside-binding mistletoe lectin, whose fine specificity is different from galectin-1, its reduced extent of binding in solid-phase assays and the disparate profile of lectin-reactive glycoproteinsreveal a non-uniform developmental alteration within the group of structural variants of beta-galactosides. Although sample preparation canaffect ligand preservation and/or presentation and thus restricts thecomparability of biochemical and histochemical results, especially for soluble reactants, the histochemical studies on frozen and paraffin-embedded sections of bovine heart, kidney and liver demonstrate that the localization of the galectin and of lectin-reactive epitopes can show a similar distribution, as seen in liver and heart, with organ-typical quantitative changes of a rather similar staining profile (heart, kidney) or notable changes in the spatial distribution (liver) in the course of development. This report emphasizes the potential value of combined monitoring of the lectin and its potential in vivo ligands to contribute to eventually unravel organ-related function(s) of a tissuelectin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 19:57:17