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Titolo:
CRYOPRESERVATION OF EMBRYOGENIC TISSUE OF A RANGE OF GENOTYPES OF SWEET-POTATO (IPOMOEA-BATATAS [L] LAM) USING AN ENCAPSULATION PROTOCOL
Autore:
BHATTI MH; PERCIVAL T; DAVEY CDM; HENSHAW GG; BLAKESLEY D;
Indirizzi:
UNIV BATH,SCH BIOL & BIOCHEM BATH BA2 7AY AVON ENGLAND UNIV BATH,SCH BIOL & BIOCHEM BATH BA2 7AY AVON ENGLAND
Titolo Testata:
Plant cell reports
fascicolo: 11, volume: 16, anno: 1997,
pagine: 802 - 806
SICI:
0721-7714(1997)16:11<802:COETOA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOMATIC EMBRYOS; SHOOT-TIPS; DEHYDRATION; SUCROSE; TRANSFORMATION; SYSTEM;
Keywords:
SWEET POTATO; CRYOPRESERVATION; SOMATIC EMBRYOGENESIS; ENCAPSULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
M.H. Bhatti et al., "CRYOPRESERVATION OF EMBRYOGENIC TISSUE OF A RANGE OF GENOTYPES OF SWEET-POTATO (IPOMOEA-BATATAS [L] LAM) USING AN ENCAPSULATION PROTOCOL", Plant cell reports, 16(11), 1997, pp. 802-806

Abstract

Embryogenic tissue of nine sweet potato [Ipomoea batatas (L. ) Lam] genotypes from Asia, Africa and the Americas was established from in vitro axillary buds on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. Embryogenic aggregates, 1.0-2.0 mm in diameter, were encapsulated in alginate gel, precultured on medium containing elevated levels of sucrose anddehydrated prior to rapid freezing in liquid nitrogen. The maximum survival of embryogenic tissue ranged from 4% to 38%, depending on the genotype. With the incorporation of a slow-cooling step, survival was generally much higher than that obtained after rapid freezing alone. Five of eight genotypes tested with this protocol gave survival percentages in excess of 55%, and a further two in excess of 33%, all after evaporative dehydration. The most effective sucrose treatment(s), however, varied with the genotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:45:48