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Titolo:
RAPID TARGETING OF NUCLEAR PROTEINS TO THE CYTOPLASM
Autore:
KLEMM JD; BEALS CR; CRABTREE GR;
Indirizzi:
STANFORD UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT DEV BIOL STANFORD CA 94305 STANFORD UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT DEV BIOL STANFORD CA 94305
Titolo Testata:
Current biology
fascicolo: 9, volume: 7, anno: 1997,
pagine: 638 - 644
SICI:
0960-9822(1997)7:9<638:RTONPT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
REV ACTIVATION DOMAIN; T-CELL ACTIVATION; VIRUS TYPE-1 REV; EXPORT SIGNAL; NF-AT; NUCLEOCYTOPLASMIC TRANSPORT; CYCLOSPORINE-A; IDENTIFICATION; LOCALIZATION; TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
J.D. Klemm et al., "RAPID TARGETING OF NUCLEAR PROTEINS TO THE CYTOPLASM", Current biology, 7(9), 1997, pp. 638-644

Abstract

Background: The transcription factor NF-ATc plays a key role in the activation of many early immune response genes and is regulated by subcellular localization, NF-ATc translocates from the cytoplasm to the nucleus in response to a rise in intracellular calcium, and immediately returns to the cytoplasm when intracellular calcium levels fall, The rapid nuclear exit of NF-ATc is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. Results: To study the nuclear export of NF-ATc, we have developed a general, non-invasive assay for the identification and study of nuclear export signals (NESs), The NES is defined by its ability to translocate a protein from the nucleus to the cytoplasm when the two are tethered by a membrane-permeable ligand, This procedure has allowed us to identifya NES within NF-ATc that functions in concert with a glycogen synthase kinase-regulated process to direct the rapid nuclear exit of NF-ATc. Conclusions: The rapid nuclear export of NF-ATc via its NES and a glycogen synthase kinase-regulated event may be an important mechanism for insulating cells from transient spikes in intracellular calcium which might otherwise lead to inappropriate activation. The assay we have developed allows the rapid identification of NESs and can be used as ageneral method for the inducible cytoplasmic export of nuclear proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 10:47:03