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Titolo:
FLASH-INDUCED CONDUCTANCE CHANGES IN CHLOROPLAST THYLAKOID LAMELLAE -A PATCH-CLAMP STUDY
Autore:
VANVOORTHUYSEN T; BULYCHEV AA; DASSEN HHA; SNEL JFH; VREDENBERG WJ;
Indirizzi:
AGR UNIV WAGENINGEN,DEPT PLANT PHYSIOL,GRAD SCH ENVIRONM PLANT SCI,ARBORETUMLAAN 4 NL-6703 BD WAGENINGEN NETHERLANDS AGR UNIV WAGENINGEN,DEPT PLANT PHYSIOL,GRAD SCH ENVIRONM PLANT SCI NL-6703 BD WAGENINGEN NETHERLANDS MOSCOW MV LOMONOSOV STATE UNIV,FAC BIOL,DEPT BIOPHYS MOSCOW 119899 RUSSIA
Titolo Testata:
Bioelectrochemistry and bioenergetics
fascicolo: 1, volume: 43, anno: 1997,
pagine: 41 - 49
SICI:
0302-4598(1997)43:1<41:FCCICT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOTOSYSTEM-II; ELECTRON-TRANSPORT; CHARGE SEPARATION; MEMBRANE-SURFACE; PROTON DIFFUSION; HIGHER-PLANTS; TURNOVER; FIELD; PHOTOSYNTHESIS; FLUORESCENCE;
Keywords:
ELECTRICAL CONDUCTANCE; PATCH-CLAMP; PEPEROMIA METALLICA; PHOTOCURRENT; THYLAKOID STRUCTURE; VOLTAGE-CLAMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
T. Vanvoorthuysen et al., "FLASH-INDUCED CONDUCTANCE CHANGES IN CHLOROPLAST THYLAKOID LAMELLAE -A PATCH-CLAMP STUDY", Bioelectrochemistry and bioenergetics, 43(1), 1997, pp. 41-49

Abstract

A patch-clamp method was used for measuring flash-induced currents (photocurrents) in Peperomia metallica chloroplasts at different holdingpotentials (V-h) in a 'whole-thylakoid' configuration. The applied holding potentials modified the kinetics of the photocurrents dramatically. A partially reversible, transient decrease in the magnitude of theholding current was observed with a minimum at 50-200 ms after the flash followed by a slow relaxation in 1-10 s. Under similar conditions the chloroplast input (seal) conductance was followed by phase-sensitive detection with sine-wave potential modulation. The seal conductancewas found to decrease transiently by 0.3-5% after a single-turnover flash and much more upon continuous illumination or repetitive flashes. The kinetics of these conductance changes were comparable to the current profile as revealed by subtracting the current traces measured in the absence and presence, respectively, of a holding potential. A major part of the conductance changes are suggested to be intimately related to alterations in the electrical properties of the lateral conductance phases along the thylakoid lamellae. The changes are extremely sensitive to agents that modify ion transport and exchange processes in the membrane and its adjoining phases, The patch-clamp method is uniquefor quantifying these conductance changes in intact chloroplasts on ams time scale. (C) 1997 Elsevier Science S.A.

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Documento generato il 05/07/20 alle ore 23:03:23