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Titolo:
DESIGN OF NEW REAGENTS ON THE BASE OF DNA DUPLEXES FOR IRREVERSIBLE INHIBITION OF TRANSCRIPTION FACTOR NF-KAPPA-B
Autore:
KOZLOV IA; KUBAREVA EA; IVANOVSKAYA MG; SHABAROVA ZA;
Indirizzi:
SALK INST BIOL STUDIES,CHEM EVALUAT LAB,POB 85800 SAN DIEGO CA 92186 MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM MOSCOW 119899 RUSSIA MOSCOW MV LOMONOSOV STATE UNIV,AN BELOZERSKY INST PHYS & CHEM BIOL MOSCOW 119899 RUSSIA
Titolo Testata:
ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT
fascicolo: 4, volume: 7, anno: 1997,
pagine: 279 - 289
SICI:
1087-2906(1997)7:4<279:DONROT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; DECOY APPROACH; P50 HOMODIMER; BINDING; OLIGONUCLEOTIDES; AFFINITY; SUBUNIT; ENZYMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
I.A. Kozlov et al., "DESIGN OF NEW REAGENTS ON THE BASE OF DNA DUPLEXES FOR IRREVERSIBLE INHIBITION OF TRANSCRIPTION FACTOR NF-KAPPA-B", ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 7(4), 1997, pp. 279-289

Abstract

The main purpose of the present work is to search for the optimal design of a DNA duplex containing an active group for crosslinking and irreversible inhibition of the transcription factor NF-kappa B. ModifiedDNA duplexes with an identical nucleotide sequence but different internucleotide phosphates replaced by the trisubstituted pyrophosphate internucleotide group were synthesized. Crosslinking of the human NF-kappa B p50 subunit with the modified DNA duplexes was carried out. It was shown that only four modified duplexes crosslinked with the NF-kappaB p50 subunit. The specificity of these reactions was confirmed. A position of the phosphate in the NF-kappa B recognition site was found where replacement on the active trisubstituted pyrophosphate group resulted in a 50% yield of crosslinking. The fact that DNA duplexes containing the trisubstituted pyrophosphate group specifically react with the NF-kappa B p50 subunit in the Escherichia coli total lysate supportsthe idea that such modified DNA can be used as high specific inhibitors for DNA-recognizing proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 00:58:42