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Titolo:
INTERLABORATORY EVALUATION OF SHORT TANDEM REPEAT TRIPLEX CTT
Autore:
KLINE MC; DUEWER DL; NEWALL P; REDMAN JW; REEDER DJ; RICHARD M;
Indirizzi:
NIST,CHEM SCI & TECHNOL LAB,DIV BIOTECHNOL GAITHERSBURG MD 20899 NIST,CHEM SCI & TECHNOL LAB,DIV ANALYT CHEM GAITHERSBURG MD 20899 CTR FORENS SCI,BIOL SECT DNA TORONTO ON M7A 2G8 CANADA
Titolo Testata:
Journal of forensic sciences
fascicolo: 5, volume: 42, anno: 1997,
pagine: 897 - 906
SICI:
0022-1198(1997)42:5<897:IEOSTR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACCURATE;
Keywords:
FORENSIC SCIENCE; ALLELE SIZING; CTTV CSF1PO; TPOX; TH01; VWA; DNA TYPING; GEL ELECTROPHORESIS; INTERLABORATORY COMPARISON; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Kline et al., "INTERLABORATORY EVALUATION OF SHORT TANDEM REPEAT TRIPLEX CTT", Journal of forensic sciences, 42(5), 1997, pp. 897-906

Abstract

An interlaboratory comparison of typing results for Short Tandem Repeats (STRs) at the GenBank loci HUMCSF1PO, HUMTPOX, HUMTH01, and HUMVWFA31 using the ''CTT triplex'' and ''CTTv quadruplex'' has been evaluated. These STRs all have a nominal four basepair (bp) repeat. Seven different samples were distributed to 41 laboratories. The 34 laboratories that returned results used a wide variety of analytical systems. Comparable results were obtained for all samples at all loci when resultswere reported as an allelic name. Raw sizing results obtained from internal-lane sizing standards differed by nearly five bp at some loci. Many different factors contribute to this observed sizing variability,including choice of sizing standards and matrix composition. Althoughsizing results can be made more comparable by locus-specific offsets or calibration to a comprehensive set of alleles at each locus, samples typed to the allelic name can now be validly compared regardless of analytical method. Interlaboratory comparison of raw allelic size remains problematic.

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Documento generato il 30/11/20 alle ore 19:51:52