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Titolo:
CHARACTERIZATION OF SIGNAL-TRANSDUCTION PATHWAYS IN HUMAN BONE-MARROWENDOTHELIAL-CELLS
Autore:
LIU ZY; GANJU RK; WANG JF; SCHWEITZER K; WEKSLER B; AVRAHAM S; GROOPMAN JE;
Indirizzi:
HARVARD UNIV,BETH ISRAEL MED CTR,SCH MED,INST MED,DIV EXPT MED,4 BLACKFAN CIRCLE BOSTON MA 02115 HARVARD UNIV,BETH ISRAEL MED CTR,SCH MED,INST MED,DIV EXPT MED BOSTONMA 02115 HARVARD UNIV,BETH ISRAEL MED CTR,SCH MED,INST MED,DIV HEMATOL ONCOL BOSTON MA 02115 FREE UNIV AMSTERDAM HOSP,DEPT HAEMATOL AMSTERDAM NETHERLANDS CORNELL UNIV MED COLL,DIV HEMATOL ONCOL NEW YORK NY 00000
Titolo Testata:
Blood
fascicolo: 6, volume: 90, anno: 1997,
pagine: 2253 - 2259
SICI:
0006-4971(1997)90:6<2253:COSPIH>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE KINASE; FOCAL ADHESION KINASE; JUN ACTIVATION DOMAIN; GROWTH-FACTOR; ONCOSTATIN-M; RECEPTOR; FLT4; PHOSPHORYLATION; EXPRESSION; MEGAKARYOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
Z.Y. Liu et al., "CHARACTERIZATION OF SIGNAL-TRANSDUCTION PATHWAYS IN HUMAN BONE-MARROWENDOTHELIAL-CELLS", Blood, 90(6), 1997, pp. 2253-2259

Abstract

Human bone marrow endothelial cells immortalized with the T antigen of SV40 (TrHBMEC) have previously been characterized by us with regard to their properties that are similar to primary marrow endothelial cells and their utility as a model system. We Flow report that TrHBMEC express a recently discovered signal transduction molecule termed RAFTK (related adhesion focal tyrosine kinase), also called Pyk2 or CAK-beta. RAFTK, the second member of the focal adhesion kinase (FAK) family, is known to be activated in response to calcium flux in neuronal cellsand integrin stimulation in megakaryocytes and B cells. We have studied the effects of cytokines on RAFTK activation in TrHBMEC, Treatment of TrHBMEC with the vascular endothelial growth factor (VEGF), as wellas the VEGF-related protein (VRP), the recently identified ligand forthe FLT-4 receptor, resulted in enhanced tyrosine phosphorylation of RAFTK. Similar changes in RAFTK phosphorylation were observed upon stimulation of TrHBMEC with basic fibroblast growth factor (bFGF) or oncostatin M (OSM). Stimulation of these cells with growth factors also resulted in an increase in RAFTK activity and the c-Jun NH2-terminal kinase (JNK). RAFTK coimmunoprecipitated with the cytoskeletal protein paxillin through its C-terminal proline-rich domain in TrHBMEC. These results suggest that, in marrow endothelium, activation of RAFTK by VEGF, VRP, OSM, and bFGF represents a new element in the signal transduction pathways used by these growth factors and likely acts to coordinatesignaling from their surface receptors to the cytoskeleton, thereby modulating cell growth and function. (C) 1997 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 13:36:30