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Titolo:
UPTAKE OF THE PHOSPHOLIPASE A(2) INHIBITOR 1-DODECYL 2-[1-C-14] OCTANAMIDO-SN-2-DEOXY GLYCERO-3-PHOSPHOCHOLINE BY PERITONEAL-MACROPHAGES
Autore:
BOURASS J; BOUCROT P; LETOURNEUX Y; GANDEMER G; PETIT JY;
Indirizzi:
INRA,LAB ETUD INTERACT MOL ALIMENTAIRES,BP 1627 F-44316 NANTES 03 FRANCE INRA,LAB ETUD INTERACT MOL ALIMENTAIRES F-44316 NANTES 03 FRANCE LAB SYNTH & ETUD SUBST NAT ACT BIOL F-17042 LA ROCHELLE 1 FRANCE FAC PHARM,LAB PHARMACOL & PHARMACOCINET F-44035 NANTES 01 FRANCE
Titolo Testata:
Journal of enzyme inhibition
fascicolo: 3, volume: 12, anno: 1997,
pagine: 227 - 240
SICI:
8755-5093(1997)12:3<227:UOTPAI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLATELET-ACTIVATING-FACTOR; ARACHIDONIC-ACID RELEASE; CELLS; LEUKOCYTES; METABOLISM; ANALOGS; PIG;
Keywords:
PHOSPHOLIPID ANALOG; PHOSPHOLIPASE A(2) INHIBITION; MACROPHAGE; ARACHIDONIC ACID; EICOSANOID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J. Bourass et al., "UPTAKE OF THE PHOSPHOLIPASE A(2) INHIBITOR 1-DODECYL 2-[1-C-14] OCTANAMIDO-SN-2-DEOXY GLYCERO-3-PHOSPHOCHOLINE BY PERITONEAL-MACROPHAGES", Journal of enzyme inhibition, 12(3), 1997, pp. 227-240

Abstract

The [C-14] phospholipid analogue 1-dodecyl-2-[l-C-14] octanamido-sn-2-deoxy glycero-3-phosphocholine was synthetized. With 2 short fatty chains linked by alkyl and amido bonds to positions 1 and 2 of the glycerophosphate backbone, it was an inhibitor of phospholipase A(2) in ionophore A23187-stimulated macrophages. Its uptake by rat peritoneal macrophages and its resistance towards phospholipases A(2) were determined at nanomolar or micromolar concentrations in the culture medium. A control substrate for phospholipases Az activity was established with the lecithin 1-octadecanoyl 2-[H-3] eicosatetraenoyl-sn-glycero-3-phosphocholine ([3H] 20:4-GPC), a source of [H-3] arachidonic acid after cleavage at position 2. Non-stimulated-or ionophore A23187-stimulated macrophages incorporated extensively the [C-14] phospholipid analogue added at 30-4000 nM. At 4000 nM which induced 50% inhibition of the phospholipase, 40% of the dose was found associated with the [C-14] phospholipids of 2 x 10(6) stimulated macrophages after 120 min incubation, while only low amounts of [ C] non-phosphorous lipids were detected. It is concluded that the [C-14] phospholipid analogue was readily takenup by the macrophages with limited hydrolysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:22:47