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Titolo:
CYTOLOGY OF CEREBROSPINAL-FLUID - ARE POLYLYSINE-COATED SLIDES USEFUL
Autore:
VANOOSTENBRUGGE RJ; ARENDS JW; BUCHHOLTZ R; TWIJNSTRA A;
Indirizzi:
UNIV HOSP MAASTRICHT,DEPT NEUROL,POB 5800 NL-6202 AZ MAASTRICHT NETHERLANDS UNIV HOSP MAASTRICHT,DEPT PATHOL,DIV CYTOPATHOL NL-6202 AZ MAASTRICHTNETHERLANDS
Titolo Testata:
Acta cytologica
fascicolo: 5, volume: 41, anno: 1997,
pagine: 1510 - 1512
SICI:
0001-5547(1997)41:5<1510:COC-AP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEPTOMENINGEAL METASTASES;
Keywords:
CEREBROSPINAL FLUID; POLYLYSINE; CYTOLOGICAL TECHNIQUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Vanoostenbrugge et al., "CYTOLOGY OF CEREBROSPINAL-FLUID - ARE POLYLYSINE-COATED SLIDES USEFUL", Acta cytologica, 41(5), 1997, pp. 1510-1512

Abstract

OBJECTIVE: To determine the effect of the use of polylysine-coated (PLC) slides for cytocentrifugation on the morphology and number of cells in cerebrospinal fluid (CSF) and its effectiveness in improving the diagnostic sensitivity of the cytologic examination of CSF. STUDY DESIGN: We assessed the morphology ann numbers of cells in 50 CSF samples obtained from 50 consecutive lumbar punctures. After centrifugation ofthe aliquots, 400 mu L was used for cytocentrifugation on PLC and plain glass slides. After Giemsa staining, random area cell counts were made for a total of 100 microscopic fields (using a 40 x objective), and cytomorphology was assessed.RESULTS: There was no difference in cytomorphologic quality between PLC and plain glass slides. The total number of cells was higher on the PLC slides (30,674 vs. 28,426; mean value per slide, 614 vs. 569), with ranges of 0-11,076 (median value, 38) and 0-10,980 (median value, 24), respectively. This difference was significant (Wilcoxon matched-pairs signed-rank test, P<.05). The higher number of cells influenced the diagnostic assessment in one case, in which only the PLC slide was conclusive. CONCLUSION: A significantly higher number of cells was found when PLC slides were used in cytopathologic assessment of CSF samples, resulting, however, in only minor improvements in diagnostic sensitivity. These findings do not warrant the routine use of PLC slides during cytocentrifugation of CSF.

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Documento generato il 05/12/20 alle ore 01:44:58