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Titolo:
CLONING OF RAT LAMININ GAMMA-1-CHAIN GENE PROMOTER REVEALS MOTIFS FORRECOGNITION OF MULTIPLE TRANSCRIPTION FACTORS
Autore:
ONEILL BC; SUZUKI H; LOOMIS WP; DENISENKO O; BOMSZTYK K;
Indirizzi:
UNIV WASHINGTON,DEPT MED,BOX 356521 SEATTLE WA 98195 UNIV WASHINGTON,DEPT MED SEATTLE WA 98195 UNIV WASHINGTON,DEPT MICROBIOL SEATTLE WA 98195
Titolo Testata:
American journal of physiology. Renal, fluid and electrolyte physiology
fascicolo: 3, volume: 42, anno: 1997,
pagine: 411 - 420
SICI:
0363-6127(1997)42:3<411:CORLGG>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLOMERULAR EPITHELIAL-CELLS; RNA-POLYMERASE-II; EXTRACELLULAR-MATRIX; MEMBRANOUS NEPHROPATHY; DNA-BINDING; CHAIN GENE; HIGH GLUCOSE; EXPRESSION; ELEMENT; GLOMERULONEPHRITIS;
Keywords:
LAMININ B2; INTERLEUKIN-1; LAMININ PROMOTER; TRANSCRIPTION INITIATION SITES; GLOMERULAR CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
B.C. Oneill et al., "CLONING OF RAT LAMININ GAMMA-1-CHAIN GENE PROMOTER REVEALS MOTIFS FORRECOGNITION OF MULTIPLE TRANSCRIPTION FACTORS", American journal of physiology. Renal, fluid and electrolyte physiology, 42(3), 1997, pp. 411-420

Abstract

We have previously shown that laminin gamma 1 (laminin B2)-chain mRNAlevels increase in response to treatment of rat glomerular epithelialcells (GEC) with the cytokine interleukin-1 beta (IL-1 beta) [C. A. Richardson, K. L. Gordon, W. G. Couser, and K. Bomsztyk. Am. J. Physiol. 268 (Renal Fluid Electrolyte Physiol. 37): F273-F278, 1995]. IL-1 beta-induced increase in laminin gamma 1-chain gene expression is likelyto be transcriptionally regulated. As the laminin gamma 1-chain gene promoter had not previously been cloned in the rat, we cloned the 5'-flanking region of this gene from a rat genomic library. Like the humanand murine laminin gamma 1-chain gene promoters, the rat laminin gamma 1-chain gene fragment spanning from nucleotides -1104 to +109, relative to the start codon, is ''GC'' rich and lacks TATA or CAAT boxes. This rat laminin gamma 1-chain gene promoter region appears to contain at least two transcription initiation sites, i.e., position -169 and -234. In transient transfections in GEC, the -1104/+35 and -1104/-15 fragments cloned upstream of a luciferase reporter gene had very little constitutive activity and were not IL-1 beta responsive. In sharp contrast, a -1104/-234 fragment exhibited constitutive activity and was IL-1 beta responsive. The -1104/-234 fragment contains motifs that recognize Sp1, BCN-1, and ApoE-B1-AP1 DNA-binding activities in GEC nuclearextracts. Collectively, the results of this study suggest that multiple inducible transcription factors may regulate laminin gamma 1-chain gene promoter activity in GEC.

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Documento generato il 29/09/20 alle ore 07:40:34