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Titolo:
AN AMINO-TERMINAL FRAGMENT OF LCRF, LCRF-(1-35), HAS THE SAME ACTIVITY AS THE NATURAL PEPTIDE
Autore:
SPANNAGEL AW; REEVE JR; LIDDLE RA; GUAN DF; GREEN GM;
Indirizzi:
UNIV TEXAS,HLTH SCI CTR,DEPT PHYSIOL,7703 FLOYD CURL DR SAN ANTONIO TX 78284 UNIV TEXAS,HLTH SCI CTR,DEPT PHYSIOL SAN ANTONIO TX 78284 UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED DURHAM NC 27710 UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,CURE DURHAM NC 27710 UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DIGEST DIS RES CTR DURHAM NC 27710
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 3, volume: 36, anno: 1997,
pagine: 754 - 758
SICI:
0193-1857(1997)36:3<754:AAFOLL>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHOLECYSTOKININ-RELEASING PEPTIDE; BASAL PANCREATIC-SECRETION; FEEDBACK-REGULATION; PLASMA CHOLECYSTOKININ; TRYPSIN-INHIBITOR; RAT; BILE; INTESTINE; PROTEIN; JUICE;
Keywords:
EXOCRINE PANCREAS; NEGATIVE FEEDBACK REGULATION; LUMINAL CHOLECYSTOKININ-RELEASING FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A.W. Spannagel et al., "AN AMINO-TERMINAL FRAGMENT OF LCRF, LCRF-(1-35), HAS THE SAME ACTIVITY AS THE NATURAL PEPTIDE", American journal of physiology: Gastrointestinal and liver physiology, 36(3), 1997, pp. 754-758

Abstract

A cholecystokinin (CCK)-releasing peptide, luminal CCK-releasing factor (LCRF), has been purified from rat jejunal secretion. Amino acid analysis and mass spectral analysis showed that the purified peptide is composed of 70-75 amino acid residues and has a mass of 8,136 Da. Microsequence analysis of LCRF yielded an amino acid sequence for the amino-terminal 41 residues. To determine the biologically active region ofthe molecule, a peptide was synthesized consisting of the amino-terminal 35 amino acids of LCRF. In this study, intraduodenal infusion of LCRF-(1-35) significantly stimulated pancreatic secretion in conscious rats. The dose-response curves to LCRF-(1-35) and to monitor peptide were similar and biphasic, with higher doses producing submaximal pancreatic secretory responses. The CCK-A receptor antagonist MK-329 abolished the pancreatic secretory response to intraduodenally infused LCRF-(1-35). These results demonstrate that LCRF biological activity is contained within the amino-terminal 35-amino acid portion of LCRF, and this fragment may be useful for investigating the role of LCRF in gastrointestinal function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 05:21:16