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Titolo:
INHIBITORY EFFECTS OF CORYMINE, AN ALKALOIDAL COMPONENT FROM THE LEAVES OF HUNTERIA-ZEYLANICA, ON GLYCINE RECEPTORS EXPRESSED IN XENOPUS OOCYTES
Autore:
LEEWANICH P; TOHDA M; MATSUMOTO K; SUBHADHIRASAKUL S; TAKAYAMA H; AIMI N; WATANABE H;
Indirizzi:
TOYAMA MED & PHARMACEUT UNIV,DEPT PHARMACOL,RES INST WAKAN YAKU ORIENTAL MED,2630 SUGITANI TOYAMA 93001 JAPAN TOYAMA MED & PHARMACEUT UNIV,DEPT PHARMACOL,RES INST WAKAN YAKU ORIENTAL MED TOYAMA 93001 JAPAN PRINCE SONGKLA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT BOT & PHARMACOGNOSY SONGKHLA 90112 THAILAND CHIBA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT CHEM CHIBA 263 JAPAN
Titolo Testata:
European journal of pharmacology
fascicolo: 3, volume: 332, anno: 1997,
pagine: 321 - 326
SICI:
0014-2999(1997)332:3<321:IEOCAA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDOLE ALKALOIDS; MESSENGER-RNA; RAT-BRAIN; ACID RECEPTORS; STEM BARK; NEURONS; THAILAND; GABA;
Keywords:
CORYMINE; XENOPUS OOCYTES; GABA(A) RECEPTORS; GLYCINE RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
P. Leewanich et al., "INHIBITORY EFFECTS OF CORYMINE, AN ALKALOIDAL COMPONENT FROM THE LEAVES OF HUNTERIA-ZEYLANICA, ON GLYCINE RECEPTORS EXPRESSED IN XENOPUS OOCYTES", European journal of pharmacology, 332(3), 1997, pp. 321-326

Abstract

We previously reported that corymine, an alkaloidal compound extracted from the leaves of Hunteria zeylanica native to Thailand, potentiated convulsions induced by either picrotoxin or strychnine. Therefore, to clarify the mechanism of action of corymine, the effects of corymineon gamma-aminobutyric acid (GABA) and glycine receptors were examined. We used Xenopus oocytes expressing these receptors and the two-electrode voltage-clamp method. The receptors expressed in oocytes injectedwith rat brain and spinal cord RNA showed the pharmacological properties of GABA(A) and glycine receptors, respectively. Corymine (1-100 muM) partially (20-30%) reduced the GABA responses in oocytes injected with rat brain RNA, while marked (up to 80%) dose-dependent reductionswere observed in the glycine responses in oocytes injected with rat spinal cord RNA. These observations suggest that corymine was more effective against the glycine receptors than the GABA receptors. The ED50 of corymine on the glycine response was 10.8 mu M. Corymine, at 30 mu M, caused a shift to the right, with a lower maximal response, of the glycine concentration-response curve. This indicated that the action of corymine on glycine receptors is neither competitive nor purely non-competitive. These observations suggest that a binding site other thanthe glycine recognition site of the glycine receptors is the site of action of corymine. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 02:48:19