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Titolo:
PHOSPHORYLATION OF THE SENDAI VIRUS M-PROTEIN IS NOT ESSENTIAL FOR VIRUS-REPLICATION EITHER IN-VITRO OR IN-VIVO
Autore:
SAKAGUCHI T; KIYOTANI K; KATO A; ASAKAWA M; FUJII Y; NAGAI Y; YOSHIDA T;
Indirizzi:
HIROSHIMA UNIV,SCH MED,DEPT BACTERIOL,MINAMI KU,1-2-3 KASUMI HIROSHIMA 734 JAPAN UNIV TOKYO,INST MED SCI,DEPT VIRAL INFECT,MINATO KU TOKYO 108 JAPAN DNAVEC RES INC TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
Virology
fascicolo: 2, volume: 235, anno: 1997,
pagine: 360 - 366
SICI:
0042-6822(1997)235:2<360:POTSVM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX PROTEIN; INFECTED-CELLS; EXPRESSION; HN; PARAMYXOVIRUSES; MULTIPLICATION; MATURATION; MUTANT; VECTOR; BINDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Sakaguchi et al., "PHOSPHORYLATION OF THE SENDAI VIRUS M-PROTEIN IS NOT ESSENTIAL FOR VIRUS-REPLICATION EITHER IN-VITRO OR IN-VIVO", Virology, 235(2), 1997, pp. 360-366

Abstract

A large proportion of intracellular Sendai virus (SeV) M proteins is phosphorylated, but in mature virions the M protein is not phosphorylated or dephosphorylated. Phosphorylated WI protein in cells is bound to the cytoskeletal components more firmly than unphosphorylated M protein. Thus it has been hypothesized that M protein phosphorylation plays an important role in the virus life cycle, especially in the step ofmaturation. Here, a transient expression-mutation experiment of the Mgene demonstrated that a change of the Ser residue al the 70th position from the N-terminus to Ala (S70A) totally abolished M protein phosphorylation, strongly suggesting that this residue is phosphorylated. The mutated M gene was then placed in the corresponding region in the cDNA plasmid which generates a full-length antigenome SeV RNA, and a mutant SeV M-S70A was successfully recovered from the cDNA. This mutant virus was indeed defective in M protein phosphorylation but did not differ at all from the wild-type SeV recovered from the parental cDNA either in the replication kinetics and plaque morphology in cultured cells or in in vivo replication and pathogenicity for mice. We thus concluded that no phosphorylation of the M protein was required for SeV replication either in vitro or in vivo. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 18:59:16