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Titolo:
SUCCESSFUL DEVELOPMENT OF AIR-DRIED MICROPLATES (HP-PLATES) FOR SUSCEPTIBILITY TESTING AGAINST HELICOBACTER-PYLORI ISOLATES
Autore:
KAWAKAMI Y; AKAHANE T; GOTOH A; OKIMURA Y; OANA K; KATSUYAMA T;
Indirizzi:
SHINSHU UNIV,SCH ALLIED MED SCI,DEPT MED TECHNOL,DIV CLIN MICROBIOL,3-1-1 ASAHI MATSUMOTO NAGANO 390 JAPAN SHINSHU UNIV,SCH MED,DEPT LAB MED MATSUMOTO NAGANO 390 JAPAN SHINSHU UNIV,SCH MED,DEPT INTERNAL MED 2 MATSUMOTO NAGANO 390 JAPAN
Titolo Testata:
Microbiology and immunology
fascicolo: 9, volume: 41, anno: 1997,
pagine: 703 - 708
SICI:
0385-5600(1997)41:9<703:SDOAM(>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAMPYLOBACTER-PYLORIDIS; DUODENAL-ULCER; THERAPY; INFECTION; AGENTS;
Keywords:
HELICOBACTER PYLORI; SUSCEPTIBILITY TEST; DRY-PLATE METHOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
Y. Kawakami et al., "SUCCESSFUL DEVELOPMENT OF AIR-DRIED MICROPLATES (HP-PLATES) FOR SUSCEPTIBILITY TESTING AGAINST HELICOBACTER-PYLORI ISOLATES", Microbiology and immunology, 41(9), 1997, pp. 703-708

Abstract

We have successfully developed and evaluated a new susceptibility testing procedure against Helicobacter pylori strains using air-dried microplates ''HP-Plates'' containing eight serially-diluted anti-H. pylori agents. HP-Plate wells were reconstituted by the inoculation of 100 mu l of H. pylori cell suspensions, After incubation at 37 C for 48 hrunder humidified microaerophilic conditions, HP-Plates were read visually with a circular mirror, We investigated the within-day reproducibility tests of HP-Plates using the six quality control (QC) strains weproposed, Of the 20 testings, determining the minimum inhibitory concentrations (MICs) of all the QC strains fell within +/-1 log2 dilutionranges. When 200 clinical isolates were tested with HP-Plates and compared with the results obtained with the modified broth macrodilution method of NCCLS, more than 90% of the MICs also fell within +/-1 log2 dilution ranges, We concluded that the HP-Plate susceptibility test method is a practical and easily applicable alternative of susceptibility testing for clinical microbiology laboratories in determining the MICs of H. pylori isolates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 10:10:26