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Titolo:
EXPRESSION AND IMMUNOGENICITY OF V-3 LOOP EPITOPES OF HIV-1, ISOLATESSC AND WMJ2, INSERTED IN SALMONELLA FLAGELLIN
Autore:
CATTOZZO EM; STOCKER BAD; RADAELLI A; MORGHEN CD; TOGNON M;
Indirizzi:
UNIV FERRARA,SCH MED,INST HISTOL & GEN EMBRYOL,VIA FOSSATO MORTARA 64-B I-44100 FERRARA ITALY UNIV FERRARA,SCH MED,INST HISTOL & GEN EMBRYOL I-44100 FERRARA ITALY UNIV FERRARA,INTERDEPT CTR BIOTECHNOL I-44100 FERRARA ITALY STANFORD UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL STANFORD CA 94305 UNIV MILAN,DEPT PHARMACOL I-20129 MILAN ITALY UNIV MILAN,INST PHARMACOL SCI I-20129 MILAN ITALY
Titolo Testata:
Journal of biotechnology
fascicolo: 3, volume: 56, anno: 1997,
pagine: 191 - 203
SICI:
0168-1656(1997)56:3<191:EAIOVL>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; RECEPTOR-MEDIATED ENTRY; ORAL IMMUNIZATION; ENVELOPE PROTEIN; B-SUBUNIT; VACCINE; NEUTRALIZATION; ANTIBODIES; GP120; TYPE-1;
Keywords:
V-3 LOOP; GP120; AROA(-) SALMONELLA; FLAGELLIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
E.M. Cattozzo et al., "EXPRESSION AND IMMUNOGENICITY OF V-3 LOOP EPITOPES OF HIV-1, ISOLATESSC AND WMJ2, INSERTED IN SALMONELLA FLAGELLIN", Journal of biotechnology, 56(3), 1997, pp. 191-203

Abstract

Synthetic oligonucleotides corresponding to specific V-3 loop portions of two HIV-1 isolates, SC and WMJ2, were expressed in the flagella of a Salmonella live-vaccine strain. Expression of the inserted epitopes in flagellin and their exposure at the surface of flagellar filaments were shown by immunoblotting and immunogold labeling with anti-flagellin (Salmonella rt) and anti-HIV-1(IIIB) V-3 loop peptide sera. Live recombinant Salmonella strains expressing either one of the two V, loop inserts were administered intraperitoneally to BALB/c mice. All these animals developed antibodies specific for the heterologous glycoprotein 120 (gp120) of HIV-I MN strain, as detected by enzyme-linked immunosorbent assays (ELISA); two of the sera had neutralizing activity against the heterologous HIV-I MN strain. Moreover, oral administration of the live Salmonella recombinant strains to mice evoked specific IgA directed against gp120. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 23:55:27