Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SWELLING-ACTIVATED GD3-SENSITIVE CATION CURRENT AND CELL-VOLUME REGULATION IN RABBIT VENTRICULAR MYOCYTES()
Autore:
CLEMO HF; BAUMGARTEN CM;
Indirizzi:
VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PHYSIOL,BOX 980551 RICHMOND VA 23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PHYSIOL RICHMOND VA23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT INTERNAL MED CARDIOL RICHMOND VA 23298
Titolo Testata:
The Journal of general physiology
fascicolo: 3, volume: 110, anno: 1997,
pagine: 297 - 312
SICI:
0022-1295(1997)110:3<297:SGCCAC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED CHICK HEART; PIG CARDIAC MYOCYTES; SENSITIVE CHLORIDE CONDUCTANCE; MECHANOSENSITIVE ION CHANNELS; DELAYED RECTIFIER K+; ATRIAL CELLS; INTRACELLULAR CHLORIDE; POTASSIUM CURRENT; AMPHOTERICIN-B; CL CHANNEL;
Keywords:
OSMOREGULATION; STRETCH-ACTIVATED CHANNELS; MECHANOELECTRICAL FEEDBACK; ARRHYTHMIAS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
H.F. Clemo e C.M. Baumgarten, "SWELLING-ACTIVATED GD3-SENSITIVE CATION CURRENT AND CELL-VOLUME REGULATION IN RABBIT VENTRICULAR MYOCYTES()", The Journal of general physiology, 110(3), 1997, pp. 297-312

Abstract

The role of swelling-activated currents in cell volume regulation is unclear. Currents elicited by swelling rabbit ventricular myocytes in solutions with 0.6-0.9X normal osmolarity, were studied using amphotericin perforated patch clamp techniques, and cell volume was examined concurrently by digital video microscopy. Graded swelling caused gradedactivation of an inwardly rectifying, time-independent cation current(I-Cir,I-swell) that was reversibly blocked by Gd3+, but I-Cir,I-swell was not detected in isotonic or hypertonic media. This current was not related to I-Kl because it was insensitive to Ba2+. The P-K/P-Na ratio for I-Cir,I-swell was 5.9 +/- 0.3, implying that inward current islargely Na+ under physiological conditions. Increasing bath K+ increased g(Cir,swell) but decreased rectification. Gd3+ block was fitted with a K-0.5 of 1.7 +/- 0.3 mu M and Hill coefficient, n, of 1.7 +/- 0.4. Exposure to Gd3+ also reduced hypotonic swelling by up to similar to30%, and block of current preceded the volume change by similar to 1 min. Gd3+-induced cell shrinkage was proportional to I-Cir,I-swell when I-Cir,I-swell was varied by graded swelling or Gd3+ concentration and was voltage dependent, reflecting the voltage dependence of I-Cir,I-swell. Integrating the blocked ion flux and volume change at -80 mV. In addition, swelling activated an outwardly rectifying Cl- current, I-Cl,I-swell. This current was absent after Cl- replacement, reversed atE-Cl, and was blocked by 1 mM 9-anthracene carboxylic acid. Block of I-Cl,I-swell provoked a 28% increase in swelling in hypotonic media. Thus, both cation and anion swelling-activated currents modulated the volume of ventricular myocytes. Besides its effects on cell volume, I-Cir,I-swell is expected to cause diastolic depolarization, Activation of I-Cir,I-swell also is likely to affect contraction and other physiological processes in myocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 03:50:36