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Titolo:
APOPTOSIS AND PROTEIN EXPRESSION AFTER FOCAL CEREBRAL-ISCHEMIA IN RAT
Autore:
LI Y; CHOPP M; POWERS C; JIANG N;
Indirizzi:
HENRY FORD HOSP,DEPT NEUROL,2799 W GRAND BLVD DETROIT MI 48202 HENRY FORD HLTH SCI CTR,DEPT NEUROL DETROIT MI 48202 OAKLAND UNIV,DEPT PHYS ROCHESTER MI 48309
Titolo Testata:
Brain research
fascicolo: 2, volume: 765, anno: 1997,
pagine: 301 - 312
SICI:
0006-8993(1997)765:2<301:AAPEAF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELAYED NEURONAL DEATH; PROGRAMMED CELL-DEATH; INTERNUCLEOSOMAL DNA FRAGMENTATION; TRANSIENT FOREBRAIN ISCHEMIA; CYCLE CHECKPOINT PATHWAY; TUMOR-SUPPRESSOR P53; GERBIL HIPPOCAMPUS; IN-SITU; ARTERY OCCLUSION; STRAND BREAKS;
Keywords:
CEREBRAL ISCHEMIA; RAT; CELL CYCLE PROTEIN; P53 RESPONSE PROTEIN; DNA DAMAGE; DNA REPAIR; APOPTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
Y. Li et al., "APOPTOSIS AND PROTEIN EXPRESSION AFTER FOCAL CEREBRAL-ISCHEMIA IN RAT", Brain research, 765(2), 1997, pp. 301-312

Abstract

We used double staining histochemistry to investigate the relationship between apoptotic cell death and selective protein expression associated with DNA damage (p53, Bax, MDM2, Gadd45), DNA repair (PCNA) and cell cycle proteins (cyclin A, cyclin D, cdk2, cdk4) in rats (n = 6; control rats, n = 5) subjected to transient (2 h) middle cerebral arteryocclusion (MCAo) and 46 h of reperfusion. Few apoptotic cells were detected in the non-ischemic hemisphere of control rats. In ischemic animals, scattered apoptotic cells were present in the ischemic core and clustered apoptotic cells were present and localized to the inner boundary zone of the ischemic core. Proteins were preferentially localizedto the cellular cytoplasm of control rats and in the non-ischemic hemisphere of rats subjected to MCAo. However, after MCAo these proteins were expressed and were preferentially localized to nuclei within the ischemic lesion. DNA damage induced proteins (wt-p53 and p53-response proteins) were preferentially expressed within apoptotic cells after ischemia. DNA repair proteins and cell cycle proteins were preferentially expressed within morphologically intact cells and in reversibly damaged cells in the ischemic areas. The selective expression of proteinsassociated with DNA damage,:DNA repair and cell cycle observed in morphologically intact cells, ischemic injured cells and apoptotic cells suggests a differential role for these proteins in cell survival and apoptosis after stroke. (C) 1997 Elsevier Science B.V.

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Documento generato il 03/06/20 alle ore 07:05:46