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Titolo:
LACK OF HIGH-AFFINITY FIBER RECEPTOR ACTIVITY EXPLAINS THE RESISTANCEOF CILIATED AIRWAY EPITHELIA TO ADENOVIRUS INFECTION
Autore:
ZABNER J; FREIMUTH P; PUGA A; FABREGA A; WELSH MJ;
Indirizzi:
UNIV IOWA,COLL MED,HOWARD HUGHES MED INST,DEPT INTERNAL MED,500 EMRB IOWA CITY IA 52242 UNIV IOWA,COLL MED,HOWARD HUGHES MED INST,DEPT INTERNAL MED IOWA CITYIA 52242 UNIV IOWA,COLL MED,DEPT PHYSIOL & BIOPHYS IOWA CITY IA 52242 UNIV IOWA,COLL MED,DEPT SURG IOWA CITY IA 52242 BROOKHAVEN NATL LAB UPTON NY 11973
Titolo Testata:
The Journal of clinical investigation
fascicolo: 5, volume: 100, anno: 1997,
pagine: 1144 - 1149
SICI:
0021-9738(1997)100:5<1144:LOHFRA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; PENTON BASE PROTEIN; CYSTIC-FIBROSIS; NASAL EPITHELIUM; TRACHEAL EPITHELIUM; COOPERATIVE BINDING; HELA-CELLS; CFTR CDNA; VECTOR; TYPE-2;
Keywords:
GENE TRANSFER; ADENOVIRUS; CYSTIC FIBROSIS; FIBER; PENTON BASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J. Zabner et al., "LACK OF HIGH-AFFINITY FIBER RECEPTOR ACTIVITY EXPLAINS THE RESISTANCEOF CILIATED AIRWAY EPITHELIA TO ADENOVIRUS INFECTION", The Journal of clinical investigation, 100(5), 1997, pp. 1144-1149

Abstract

Although recombinant adenoviruses are attractive vectors for gene transfer to airway epithelia, they have proven to be relatively inefficient, To investigate the mechanisms of adenovirus-mediated gene transferto airway epithelia, we examined the role of adenovirus fiber and penton base, the two proteins involved in attachment to and entry of virus into the cell, We used human airway epithelia grown under conditionsthat allow differentiation and development of a ciliated apical surface that closely resembles the in vivo condition. We found that addition of fiber protein inhibited virus binding and vector-mediated gene transfer to immature airway epithelia, as well as to primary cultures ofrat hepatocytes and HeLa cells, However, fiber protein had no effect on vector binding and gene transfer to ciliated airway epithelia, We obtained similar results with addition of penton base protein: the protein inhibited gene transfer to immature epithelia, whereas there was no effect with ciliated epithelia, Moreover, infection was not attenuated with an adenovirus containing a mutation in penton base that prevents the interaction with cell surface integrins. These data suggest that the receptors required for efficient infection by adenovirus are either not present or not available on the apical surface of ciliated human airway epithelia, The results explain the reason for inefficient gene transfer and suggest approaches for improvement.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 23:55:26