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Titolo:
ALTERATIONS IN ORNITHINE DECARBOXYLASE CHARACTERISTICS ACCOUNT FOR TOLERANCE OF TRYPANOSOMA-BRUCEI-RHODESIENSE TO D,L-ALPHA-DIFLUOROMETHYLORNITHINE
Autore:
ITEN M; METT H; EVANS A; ENYARU JCK; BRUN R; KAMINSKY R;
Indirizzi:
SWISS TROP INST,POB 4002 BASEL SWITZERLAND SWISS TROP INST BASEL SWITZERLAND CIBA BASEL SWITZERLAND LIVESTOCK HLTH RES INST TORORO UGANDA
Titolo Testata:
Antimicrobial agents and chemotherapy
fascicolo: 9, volume: 41, anno: 1997,
pagine: 1922 - 1925
SICI:
0066-4804(1997)41:9<1922:AIODCA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
DL-ALPHA-DIFLUOROMETHYLORNITHINE; BLOOD-STREAM TRYPOMASTIGOTES; GAMBIENSE SLEEPING SICKNESS; RESISTANCE; FORMS; SUSCEPTIBILITY; METABOLISM; EXPRESSION; INHIBITION; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
M. Iten et al., "ALTERATIONS IN ORNITHINE DECARBOXYLASE CHARACTERISTICS ACCOUNT FOR TOLERANCE OF TRYPANOSOMA-BRUCEI-RHODESIENSE TO D,L-ALPHA-DIFLUOROMETHYLORNITHINE", Antimicrobial agents and chemotherapy, 41(9), 1997, pp. 1922-1925

Abstract

Ornithine decarboxylase (ODC), the target enzyme of D,L-alpha-difluoromethylornithine (DFMO), was investigated in four DFMO-tolerant Trypanosoma brucei rhodesiense isolates from Fast Africa and two DFMO-susceptible T. b. gambiense isolates from West Africa, Neither drug uptake nor inhibition of ODC activity by DEMO in cellular extracts differed inthe two trypanosome subspecies. However, the specific ODC activity ofthe cellular extracts was three times as high in T. b. rhodesiense isolates as in T. b, gambiense isolates. Furthermore, a significant difference in the turnover rate of ODC was observed. The time required to induce a 50% reduction of T. b. rhodesiense ODC activity under cycloheximide pressure (tentative half-life) was about 4.3 h, whereas that required for T. b. gambiense ODC was longer than 18 h. We concluded thatthe higher specific ODC activity and faster enzyme turnover contributed to a substantial degree to the DFMO tolerance observed in the East African T. b. rhodesiense isolates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:28:28