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Titolo:
PROTEIN-KINASE-C IS REQUIRED FOR THE DISAPPEARANCE OF MPF UPON ARTIFICIAL ACTIVATION IN MOUSE EGGS
Autore:
COLONNA R; TATONE C; FRANCIONE A; ROSATI F; CALLAINI G; CORDA D; DIFRANCESCO L;
Indirizzi:
UNIV AQUILA,DEPT BIOMED SCI & TECHNOL,VIA VETOIO,LOC COPPITO I-67100 LAQUILA ITALY UNIV SIENA,DEPT EVOLUT BIOL I-53100 SIENA ITALY IST RIC FARMACOL MARIO NEGRI,CONSORZIO MARIO NEGRI SUD,DEPT CELL BIOL& ONCOL I-66030 SANTA MARIA IMBAR ITALY
Titolo Testata:
Molecular reproduction and development
fascicolo: 2, volume: 48, anno: 1997,
pagine: 292 - 299
SICI:
1040-452X(1997)48:2<292:PIRFTD>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; INTRACELLULAR FREE CALCIUM; XENOPUS-LAEVIS; CALPHOSTIN-C; M-PHASE; 6-DIMETHYLAMINOPURINE 6-DMAP; OOCYTE MATURATION; PHORBOL ESTER; INHIBITOR; METAPHASE;
Keywords:
CALCIUM; PROTEIN KINASE C; EGG ACTIVATION; M-PHASE PROMOTING FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
R. Colonna et al., "PROTEIN-KINASE-C IS REQUIRED FOR THE DISAPPEARANCE OF MPF UPON ARTIFICIAL ACTIVATION IN MOUSE EGGS", Molecular reproduction and development, 48(2), 1997, pp. 292-299

Abstract

The aim of the present study was to investigate the implication of protein kinase C (PKC) in the mouse egg activation process. We used OAG (1-oleoyl-2-acetyl-sn-glycerol) as a PKC activator, calphostin C as a specific PKC inhibitor, and the calcium ionophore A23187 as a standardparthenogenetic agent. The exposure of zona-free eggs to 150 mu M or 50 mu M OAG for 10 min resulted in meiosis II completion in similar to80% of instances. By contrast, at a lower concentration (25 mu M), the PKC stimulator was ineffective as parthenogenetic agent. Shortly after the application of 150 mu M OAG, the cylosolic Ca2+ concentration ([Ca2+](i)) increased transiently in all the eggs examined, whereas after the addition of 50 mu M OAG, [Ca2+](i) remained unchanged for at least 20 min. During this period, the activity of M-phase promoting factor (MPF) dramatically decreased and most of the eggs entered anaphase except when the PKC was inhibited by calphostin C. Similarly, MPF inactivation and meiosis resumption were prevented in calphostin C-loaded eggs following treatment with A23187, even though the ionophore induced Ca2+ signalling was not affected. Taken together, our results indicate that stimulation of PKC is a sufficient and necessary event to induce meiosis resumption in mouse eggs and strongly suggest that, in thisspecies, the mechanism by which a transient calcium burst triggers MPF inactivation involves a PKC-dependent pathway. (C) 1997 Wiley-Liss Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:36:14