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Titolo:
DIVERSE UNCULTIVATED BACTERIAL GROUPS FROM SOILS OF THE ARID SOUTHWESTERN UNITED-STATES THAT ARE PRESENT IN MANY GEOGRAPHIC REGIONS
Autore:
KUSKE CR; BARNS SM; BUSCH JD;
Indirizzi:
LOS ALAMOS NATL LAB,DIV LIFE SCI,ENVIRONM MOL BIOL GRP,M888 LOS ALAMOS NM 87545
Titolo Testata:
Applied and environmental microbiology
fascicolo: 9, volume: 63, anno: 1997,
pagine: 3614 - 3621
SICI:
0099-2240(1997)63:9<3614:DUBGFS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENES; POLYMERASE CHAIN-REACTION; 16S RDNA ANALYSIS; PHYLOGENETIC ANALYSIS; PCR AMPLIFICATION; DATABASE PROJECT; DNA EXTRACTION; MICROBIAL DNA; MICROORGANISMS; SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
C.R. Kuske et al., "DIVERSE UNCULTIVATED BACTERIAL GROUPS FROM SOILS OF THE ARID SOUTHWESTERN UNITED-STATES THAT ARE PRESENT IN MANY GEOGRAPHIC REGIONS", Applied and environmental microbiology, 63(9), 1997, pp. 3614-3621

Abstract

We have performed a phylogenetic survey of microbial species present in two soils from northern Arizona. Microbial DNA was purified directly from soil samples and subjected to PCR amplification with primers specific for bacterial 16S rRNA gene sequences (rDNAs). Clone libraries from the two soils were constructed. and 60 clone inserts were partially sequenced, Phylogenetic analysis of these sequences revealed extensive diversity. Most of the analyzed sequences (64%) fell into five novel clusters having no known cultured members. Extensive analysis of 10nearly full-length rDNAs from clones representative of the novel groups indicated that four of the five groups probably cluster into a large ''supergroup'' which is as distinct from currently recognized bacterial divisions as the latter are from each other. From this we postulate the existence of a major bacterial lineage, previously known only from a single cultured representative, whose diversity and ecology we are only beginning to explore. Analysis of our data and that from other rDNA sequence-based studies of soils from different geographic regionsshows considerable overlap of sequence types. Taken together. these groups encompass most of the novel rDNA sequences recovered in each comparable analysis reported to date, despite large differences in soil types and geographic sources. Our results indicate that members of these new groups comprise a phylogenetically diverse, geographically widespread, and perhaps numerically important component of the soil microbiota.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:51:59