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Titolo:
TRANSIENT ELECTRIC BIREFRINGENCE STUDY OF INTERMEDIATE FILAMENT FORMATION FROM VIMENTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN
Autore:
KOOIJMAN M; BLOEMENDAL M; TRAUB P; VANGRONDELLE R; VANAMERONGEN H;
Indirizzi:
FREE UNIV AMSTERDAM,DEPT PHYS & ASTRON,DE BOELELAAN 1081 NL-1081 HV AMSTERDAM NETHERLANDS FREE UNIV AMSTERDAM,DEPT PHYS & ASTRON NL-1081 HV AMSTERDAM NETHERLANDS FREE UNIV AMSTERDAM,INST MOL BIOL SCI NL-1081 HV AMSTERDAM NETHERLANDS MAX PLANCK INST CELL BIOL D-68526 LADENBURG GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 36, volume: 272, anno: 1997,
pagine: 22548 - 22555
SICI:
0021-9258(1997)272:36<22548:TEBSOI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMICAL CROSS-LINKING; COILED-COIL MOLECULES; INTEGRAL-EQUATIONS; KERATIN FILAMENTS; EPIDERMAL KERATIN; ROD DOMAIN; DESMIN; MOUSE; MECHANISM; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
M. Kooijman et al., "TRANSIENT ELECTRIC BIREFRINGENCE STUDY OF INTERMEDIATE FILAMENT FORMATION FROM VIMENTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN", The Journal of biological chemistry, 272(36), 1997, pp. 22548-22555

Abstract

Mg2+-induced polymerization of type III intermediate filament proteins vimentin and glial fibrillary acidic protein was studied by transient electric birefringence. In the absence of MgCl2 we found a net permanent dipole moment, similar to 45-nm-long dimers for vimentin, similarto 65-nm-long tetramers, hexamers, and possibly octamers for both proteins, and 100-nm aggregates for glial fibrillary acidic protein. Controlled oligomerization occurred after the addition of MgCl2. Although the solutions contained (small) aggregates of different sizes, more orless discrete steps in polymer formation were observed, and it was possible to discriminate between an increase in width and length. At thefirst stage of polymerization (in 0.3 mM MgCl2 for vimentin and 0.2 mM MgCl2 for glial fibrillary acidic protein), the permanent dipole moment disappeared without a change in length of the particles. At higherMgCl2 concentrations, structures of approximately 100 nm were formed,which strongly tended to laterally assemble into full-width intermediate filament structures consisting of about 32 monomers. This contrasts with previous models where first full-width (similar to 10-nm) aggregates are formed, which then increase in length. Subsequently, two discrete elongation steps of 35 nm are observed that increase the length to 135 and 170 nm, respectively. Possible structural models are suggested for the polymerization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 03:00:25