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Titolo:
CORTICOTROPIN-RELEASING FACTOR AND GLUCOCORTICOID RECEPTOR (GR) GENE-EXPRESSION IN THE PARAVENTRICULAR NUCLEUS OF IMMUNE-CHALLENGED TRANSGENIC MICE EXPRESSING TYPE-II GR ANTISENSE RIBONUCLEIC-ACID
Autore:
LAFLAMME N; BARDEN N; RIVEST S;
Indirizzi:
CHUL,RES CTR,MOL ENDOCRINOL LAB,2705 BOUL LAURIER LAVAL PQ G1V 4G2 CANADA CHUL,RES CTR,MOL ENDOCRINOL LAB LAVAL PQ G1V 4G2 CANADA CHUL,RES CTR,NEUROSCI LAB LAVAL PQ G1V 4G2 CANADA UNIV LAVAL,DEPT PHYSIOL LAVAL PQ G1V 4G2 CANADA
Titolo Testata:
Journal of molecular neuroscience
fascicolo: 3, volume: 8, anno: 1997,
pagine: 165 - 179
SICI:
0895-8696(1997)8:3<165:CFAGR(>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMEDIATE-EARLY GENES; PITUITARY-ADRENAL AXIS; ACUTE-PHASE RESPONSE; FACTOR MESSENGER-RNA; RAT-BRAIN; C-FOS; INSITU HYBRIDIZATION; NGFI-B; ANTERIOR-PITUITARY; PORTAL CIRCULATION;
Keywords:
ACUTE-PHASE RESPONSE; ANTISENSE KNOCKDOWN TECHNOLOGY; C-FOS; ENDOTOXIN; HPA AXIS; NEUROENDOCRINOLOGY; GLUCOCORTICOIDS; IN SITU HYBRIDIZATION; IMMUNOCYTOCHEMISTRY; STRESS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
N. Laflamme et al., "CORTICOTROPIN-RELEASING FACTOR AND GLUCOCORTICOID RECEPTOR (GR) GENE-EXPRESSION IN THE PARAVENTRICULAR NUCLEUS OF IMMUNE-CHALLENGED TRANSGENIC MICE EXPRESSING TYPE-II GR ANTISENSE RIBONUCLEIC-ACID", Journal of molecular neuroscience, 8(3), 1997, pp. 165-179

Abstract

The purpose of this study was to investigate the effect of the immuneactivator lipopolysaccharide (LPS) on the expression of corticotropin-releasing factor (CRF) and glucocorticoid receptor (GR) mRNA in the paraventricular nucleus (PVN) of transgenic mice with impaired GR function caused by endogenous expression of GR antisense RNA. At 3 and 8 wkof age, control and transgenic mice were sacrificed 4.5 h after a single ip administration of LPS (100 mu g/100 g of body wt) or vehicle. Frozen brains were mounted on a microtome and cut in 20-mu m sections. mRNAs encoding CRF and GR were assayed by in situ hybridization histochemistry using S-35-labeled riboprobes, and localization of Fos-immunoreactive (Fos-ir) nuclei was determined by immunocytochemistry. Basal expression of CRF mRNA in the PVN, central nucleus of the amygdala (CeA), and geniculate complex (GN) was similar in the control and transgenic mice. LPS induced a comparable neuronal activation in the PVN of control and transgenic mice as revealed by the number of Fos-ir neurons. Moreover, the endotoxin caused a significant increase in the CRF mRNA levels within the PVN and CeA, an effect observed in both animal models. The endotoxin did not notably modulate CRF expression in other regions, such as GN. Although GR mRNA was expressed in the PVN of control mice under basal conditions, this transcript was not detected in this hypothalamic structure in LPS-treated and transgenic animals. This indicated that endogenous Type ii GR mRNA is decreased in the PVN of mice expressing Type IT GR antisense RNA and that gene is downregulated by LPS. Hybridization signal for CRF and GR transcripts was not notably altered by the age of mice. These results provide evidence that the basal expression of CRF and the increase of neuroendocrine CRF transcription in response to immunogenic challenges are not significantly affected by impairment of the Type II GR function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:30:06