Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MOLECULAR CHARACTERIZATION OF THE GENES INVOLVED IN O-ANTIGEN MODIFICATION, ATTACHMENT, INTEGRATION AND EXCISION IN SHIGELLA-FLEXNERI BACTERIOPHAGE-SFV
Autore:
HUAN PT; BASTIN DA; WHITTLE BL; LINDBERG AA; VERMA NK;
Indirizzi:
AUSTRALIAN NATL UNIV,SCH LIFE SCI,FAC SCI,DIV BIOCHEM & MOL BIOL CANBERRA ACT 0200 AUSTRALIA AUSTRALIAN NATL UNIV,SCH LIFE SCI,FAC SCI,DIV BIOCHEM & MOL BIOL CANBERRA ACT 0200 AUSTRALIA KAROLINSKA INST,DEPT IMMUNOL MICROBIOL PATHOL & INFECT DIS,DIV CLIN BACTERIOL STOCKHOLM SWEDEN
Titolo Testata:
Gene
fascicolo: 2, volume: 195, anno: 1997,
pagine: 217 - 227
SICI:
0378-1119(1997)195:2<217:MCOTGI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; ESCHERICHIA-COLI; HOST FACTOR; POLYACRYLAMIDE GELS; LAMBDA; INT; LIPOPOLYSACCHARIDE; DNA; BIOSYNTHESIS; PHAGE;
Keywords:
SHIGELLA FLEXNERI; BACTERIOPHAGE SFV; GLUCOSYL TRANSFERASE GENE; ATTACHMENT SITE; INTEGRATION; EXCISION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
P.T. Huan et al., "MOLECULAR CHARACTERIZATION OF THE GENES INVOLVED IN O-ANTIGEN MODIFICATION, ATTACHMENT, INTEGRATION AND EXCISION IN SHIGELLA-FLEXNERI BACTERIOPHAGE-SFV", Gene, 195(2), 1997, pp. 217-227

Abstract

Bacteriophage SN is a temperate phage of Shigella flexneri responsible for converting serotype Y (3,4) to serotype 5a (V; 3,4) through its glucosyl transferase gene. The glucosyl transferase (gtr) gene of SfV has been cloned and shown to partially convert S. flexneri serotype Y to serotype 5a. In this study, we found that the serotype-converting region of SfV was approximately 2.5 kb in length containing three continuous ORFs. The recombinant strain carrying the three complete ORFs expressed the type V and group antigen 3,4, both indistinguishable from that of S. flexneri 5a wild-type strain. The interruption of orf5 or orf6 gave partial conversion in the S. flexneri recombinant strain indicated by the incomplete replacement of group antigen 3,4. The region adjacent to the serotype-conversion genes was found to be identical to the attP-int-xis region of phage P22. Altogether, an approximately 2.2-kb sequence covering a portion of the serotype-conversion (approximately 500 nt)-attP-int-xis regions of SfV was remarkably similar to thatof P22. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 09:13:47