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Titolo:
SHIGELLA-FLEXNERI TYPE-SPECIFIC ANTIGEN .5. CLONING, SEQUENCING AND CHARACTERIZATION OF THE GLUCOSYL TRANSFERASE GENE OF TEMPERATE BACTERIOPHAGE-SFV
Autore:
HUAN PT; WHITTLE BL; BASTIN DA; LINDBERG AA; VERMA NK;
Indirizzi:
AUSTRALIAN NATL UNIV,SCH LIFE SCI,FAC SCI,DIV BIOCHEM & MOL BIOL CANBERRA ACT 0200 AUSTRALIA AUSTRALIAN NATL UNIV,SCH LIFE SCI,FAC SCI,DIV BIOCHEM & MOL BIOL CANBERRA ACT 0200 AUSTRALIA KAROLINSKA INST,DEPT IMMUNOL MICROBIOL PATHOL & INFECT DIS,DIV CLIN BACTERIOL STOCKHOLM SWEDEN
Titolo Testata:
Gene
fascicolo: 2, volume: 195, anno: 1997,
pagine: 207 - 216
SICI:
0378-1119(1997)195:2<207:STA.CS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCES; CLONES BINDING; LIVE VACCINE; TAIL; P2; STRAINS; IMMUNOGENICITY; CONSTRUCTION; DIARRHEA; PROTEIN;
Keywords:
BACTERIOPHAGE SFV; GLUCOSYL TRANSFERASE GENE; PHAGE CONVERSION; SHIGELLA FLEXNERI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
P.T. Huan et al., "SHIGELLA-FLEXNERI TYPE-SPECIFIC ANTIGEN .5. CLONING, SEQUENCING AND CHARACTERIZATION OF THE GLUCOSYL TRANSFERASE GENE OF TEMPERATE BACTERIOPHAGE-SFV", Gene, 195(2), 1997, pp. 207-216

Abstract

With lysogeny by bacteriophage SN, Shigella flexneri serotype Y is converted to serotype 5a. The glucosyl transferase gene (gtr) from bacteriophage SN of S. flexneri, involved in serotype-specific conversion, was cloned and characterized. The DNA sequence of a 3.7 kb EcoRI-BamHIfragment of bacteriophage SfV which includes the gtr gene was determined. This gene, encoding a polypeptide of 417 aa with 47.67 kDa molecular mass, caused partial serotype conversion of S. flexneri from serotype Y to type V antigen as demonstrated by Western blotting and the sensitivity of the hybrid strain to phage Sf6. The deduced protein of the partially sequenced open reading frame upstream of the gtr showed similarity to various glycosyl transferases of other bacteria. Orf3, separated from the gtr by a non-coding region and transcribed convergently, codes for a 167 aa (18.8 kDa) protein found to have homology with tail fibre genes of phage lambda and P2. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 09:56:56