Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CLONING AND EXPRESSION OF CDNA-ENCODING A MITOGEN-ACTIVATED PROTEIN-KINASE FROM A PHYTOPATHOGENIC FILAMENTOUS FUNGUS
Autore:
LI DX; ROGERS L; KOLATTUKUDY PE;
Indirizzi:
OHIO STATE UNIV,1060 CARMACK RD COLUMBUS OH 43210 OHIO STATE UNIV COLUMBUS OH 43210
Titolo Testata:
Gene
fascicolo: 2, volume: 195, anno: 1997,
pagine: 161 - 166
SICI:
0378-1119(1997)195:2<161:CAEOCA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; F-SP-PISI; APPRESSORIUM FORMATION; NECTRIA-HAEMATOCOCCA; SIGNAL-TRANSDUCTION; INDUCTION; TYROSINE; CUTINASE; PROMOTER; FAMILY;
Keywords:
FUSARIUM SOLANI; MITOGEN-ACTIVATED PROTEIN KINASE; PHOSPHORYLATION; PCR; IMMUNOBLOT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
D.X. Li et al., "CLONING AND EXPRESSION OF CDNA-ENCODING A MITOGEN-ACTIVATED PROTEIN-KINASE FROM A PHYTOPATHOGENIC FILAMENTOUS FUNGUS", Gene, 195(2), 1997, pp. 161-166

Abstract

We have cloned a mitogen-activated protein kinase (MAPK) designated Fusarium solani sp. pisi mitogen-activated protein kinase (FsMAPK) fromthe phytopathogenic filamentous fungus F. solani f. sp. pisi T8 strain. A single open reading frame (ORF) of 1068 bp encoding a polypeptideof 355 amino acids (aa) with a predicted molecular mass of 41 194 Da was found in the cloned 1583-bp cDNA insert. FsMAPK is highly homologous to SPK1 of fission yeast, FUS3 of budding yeast, MsERK1 of alfalfa,Sur-1 of nematode, and hERK1 of human. That this gene is expressed inF. solani f. sp. pisi was shown by the finding that immunoblot of thefungal extracts with anti-FsMAPK antibodies (Ab) raised in a rabbit against the FsMAPK, expressed in Escherichia coli (E. coli), detected the corresponding protein. DNA blot analysis indicated that Fsmapk is present as a single copy in the fungal genome. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 08:51:05