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Titolo:
AUTOMATED-DETERMINATION OF CLOZAPINE AND MAJOR METABOLITES IN SERUM AND URINE
Autore:
WEIGMANN H; BIERBRAUER J; HARTTER S; HIEMKE C;
Indirizzi:
UNIV MAINZ,DEPT PSYCHIAT,UNTERE ZAHLBACHER STR 8 D-55131 MAINZ GERMANY UNIV MAINZ,DEPT PSYCHIAT D-55131 MAINZ GERMANY
Titolo Testata:
Therapeutic drug monitoring
fascicolo: 4, volume: 19, anno: 1997,
pagine: 480 - 488
SICI:
0163-4356(1997)19:4<480:AOCAMM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; REFRACTORY SCHIZOPHRENIC-PATIENTS; HUMAN-PLASMA; ULTRAVIOLET DETECTION; N-DESMETHYLCLOZAPINE; ANTIPSYCHOTIC AGENT; CLINICAL-RESPONSE; DRUGS; NORCLOZAPINE;
Keywords:
CLOZAPINE; CLOZAPINE METABOLISM; COLUMN SWITCHING; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); SERUM; URINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
H. Weigmann et al., "AUTOMATED-DETERMINATION OF CLOZAPINE AND MAJOR METABOLITES IN SERUM AND URINE", Therapeutic drug monitoring, 19(4), 1997, pp. 480-488

Abstract

Clozapine is an atypical neuroleptic that is increasingly used for the treatment of schizophrenia. An automated method was developed for the routine quantification of clozapine and its major metabolites, N-desmethylclozapine and clozapine N-oxide, in human serum and urine by column switching and online high-performance liquid chromatography with ultraviolet detection. The method included adsorption of clozapine and its metabolites on a cyanopropyl-coated clean-up column (10 mu m; 10 mm x 4.0 mm ID), washing interfering serum constituents to waste by deionized water, and, after column switching, separation on C18 ODS Hypersil reversed-phase material (5 mu m; 250 mm x 4.6 mm ID). The compounds of interest were separated and eluted in fewer than 20 minutes, using a mobile phase consisting of 37.5 acetonitril:62.5 water, containing0.4% (vol/vol) tetramethylethylenediamine and adjusted to pH 6.5 withconcentrated acetic acid. Ultraviolet-detection was performed at 354 nm. The determinations exhibited linearity between detector signal anddrug concentrations in a range from 5 ng/ml to 50 mu g/ml. As little as 10 ng/ml of clozapine and 20 or 30 ng/ml of the metabolites was quantifiable. Interferences with other psychotropic drugs, serum, or urine constituents were negligible. The automated procedure enables the analysis of clozapine and metabolites in serum or urine in less than 1 hour.

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Documento generato il 25/01/20 alle ore 19:42:40