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Titolo:
PROTEOME ANALYSIS OF SACCHAROMYCES-CEREVISIAE - A METHODOLOGICAL OUTLINE
Autore:
FEY SJ; NAWROCKI A; LARSEN MR; GORG A; ROEPSTORFF P; SKEWS GN; WILLIAMS R; LARSEN PM;
Indirizzi:
ODENSE UNIV,CTR PROTEOME ANAL LIFE SCI,INT SCI PK,FORSKEPK 10 DK-5230ODENSE M DENMARK TECH UNIV MUNICH,LEHRSTUHL ALLGEMEINE LEBENSMITTELCHEM D-8050 FREISING GERMANY ODENSE UNIV,DEPT MOL BIOL DK-5230 ODENSE DENMARK LIFE SCI RESOURCES LTD,INNOVAT CTR CAMBRIDGE ENGLAND
Titolo Testata:
Electrophoresis
fascicolo: 8, volume: 18, anno: 1997,
pagine: 1361 - 1372
SICI:
0173-0835(1997)18:8<1361:PAOS-A>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
2-DIMENSIONAL GEL-ELECTROPHORESIS; IMMOBILIZED PH GRADIENTS; FLIGHT MASS-SPECTROMETRY; SEQUENCE DATABASES; POLYACRYLAMIDE GELS; PROTEINS; IDENTIFICATION; EXPRESSION; STATE; MAPS;
Keywords:
PROTEOME ANALYSIS; SACCHAROMYCES CEREVISIAE; 2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; MASS SPECTROMETRY; FLUORESCENT LABELING OF PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
S.J. Fey et al., "PROTEOME ANALYSIS OF SACCHAROMYCES-CEREVISIAE - A METHODOLOGICAL OUTLINE", Electrophoresis, 18(8), 1997, pp. 1361-1372

Abstract

Proteome analysis offers a unique means of identifying important proteins, characterizing their modifications and beginning to describe their function. This is achieved through the combination of two technologies: protein separation and selection by two-dimensional gel electrophoresis, and protein identification and characterization by mass spectrometry. This methodological outline sketches the strengths and weaknesses of the two central technologies used, and provides both practical tips and the theoretical background for their utilization. One application of these technologies is illustrated by the characterization of genes, revealed by sequencing, but which have no -or only weak homology-to any other known genes. Other applications, for example the identification of protein markers for particular human diseases, are only referred to. The aim of the article is thus to provide the basis for a sound understanding of the full potential and limitations of proteome analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:22:01