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Titolo:
IMMUNOREACTIVITY OF CYCLIN D1 CDK4 IN NEURONS AND OLIGODENDROCYTES AFTER FOCAL CEREBRAL-ISCHEMIA IN RAT/
Autore:
LI Y; CHOPP M; POWERS C; JIANG N;
Indirizzi:
HENRY FORD HOSP,DEPT NEUROL,2799 W GRAND BLVD DETROIT MI 48202 HENRY FORD HLTH SCI CTR,DEPT NEUROL DETROIT MI 00000 OAKLAND UNIV,DEPT PHYS ROCHESTER MI 48063
Titolo Testata:
Journal of cerebral blood flow and metabolism
fascicolo: 8, volume: 17, anno: 1997,
pagine: 846 - 856
SICI:
0271-678X(1997)17:8<846:IOCDCI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSIENT FOREBRAIN ISCHEMIA; INTERNUCLEOSOMAL DNA FRAGMENTATION; PROGRAMMED CELL-DEATH; IN-SITU; ARTERY OCCLUSION; NERVOUS-SYSTEM; ADULT-RAT; APOPTOSIS; D1; HIPPOCAMPUS;
Keywords:
CYCLIN D1/CDK4; APOPTOSIS; NEURONS; OLIGODENDROGLIA; MIDDLE CEREBRAL ARTERY OCCLUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
Y. Li et al., "IMMUNOREACTIVITY OF CYCLIN D1 CDK4 IN NEURONS AND OLIGODENDROCYTES AFTER FOCAL CEREBRAL-ISCHEMIA IN RAT/", Journal of cerebral blood flow and metabolism, 17(8), 1997, pp. 846-856

Abstract

We investigated the expression of cyclin D1 and its kinase, cdk4, after induction of focal cerebral ischemia in the rat. Brain from rats (n= 6) subjected to 2 hours of transient middle cerebral artery occlusion and 46 hours of reperfusion, and control sham-operated (n = 3) and normal (n = 2) rats were processed for dual label immunohistochemical study for cellular identification of the expression of these cell cycle proteins. Antibodies raised against microtubule-associated protein 2and neuronal specific enolase for neurons, glial fibrillary acidic protein for astrocytes, myelin basic protein for oligodendrocytes and lectin histochemical study with the B4-isolectin for microglia were usedfor cell type identification. Double staining for DNA fragmentation detection (TUNEL) and expression of cyclin D1 and cdk4 also was performed. Cyclin D1 and cdk4 were selectively expressed in morphologically intact or altered neurons and oligodendrocytes localized to the ischemic tissue. Apoptotic cells were not immunoreactive to cyclin D1 and cdk3 at 46 hours after 2 hours of middle cerebral artery occlusion. The selective expression of cell cycle proteins observed in nonapoptotic ischemic postmitotic neurons and oligodendrocytes suggests a role for these proteins in cell survival after transient focal cerebral ischemia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:43:01