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Titolo:
IMMOBILIZATION OF HORSERADISH-PEROXIDASE ONTO A COMPOSITE MEMBRANE OFREGENERATED SILK FIBROIN AND POLYVINYL-ALCOHOL AND ITS APPLICATION TOA NEW METHYLENE BLUE-MEDIATING SENSOR FOR HYDROGEN-PEROXIDE
Autore:
LIU YC; QIAN JH; FU XL; LIU HY; DENG JQ; YU TY;
Indirizzi:
FUDAN UNIV,DEPT MACROMOL SCI SHANGHAI 200433 PEOPLES R CHINA FUDAN UNIV,DEPT MACROMOL SCI SHANGHAI 200433 PEOPLES R CHINA FUDAN UNIV,DEPT CHEM,LAB MOL ENGN POLYMERS SHANGHAI 200433 PEOPLES R CHINA
Titolo Testata:
Enzyme and microbial technology
fascicolo: 3, volume: 21, anno: 1997,
pagine: 154 - 159
SICI:
0141-0229(1997)21:3<154:IOHOAC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-TRANSFER; REDUCTION; SHUTTLE; OXIDASE; CARBON; ASSAY;
Keywords:
SENSOR; POLYVINYL ALCOHOL; REGENERATED SILK FIBROIN; METHYLENE BLUE (NEW); HORSERADISH PEROXIDASE; HYDROGEN PEROXIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
Y.C. Liu et al., "IMMOBILIZATION OF HORSERADISH-PEROXIDASE ONTO A COMPOSITE MEMBRANE OFREGENERATED SILK FIBROIN AND POLYVINYL-ALCOHOL AND ITS APPLICATION TOA NEW METHYLENE BLUE-MEDIATING SENSOR FOR HYDROGEN-PEROXIDE", Enzyme and microbial technology, 21(3), 1997, pp. 154-159

Abstract

Horseradish peroxidase (HRP) was effectively in a novel composite membrane of polyvinyl alcohol (PVA) and regenerated silk fibroin (RSF). IR and scanning electron microscopy were employed to provide a useful insight into the structure and morphology of the immobilized HRP composite membrane. A new methylene blue-mediating sensor for hydrogen peroxide was constructed which was based on the composite membrane as an immobilization matrix for HRP. Cyclic voltammetry and amperometric measurements were used to demonstrate the efficiency of the new methylene blue mediating an electron transfer between the immobilized HRP and a glassy carbon electrode in bioelectrocatalytic reduction of hydrogen peroxide. Performance and characteristics of the sensor were evaluated with regard to response time, detection limit, selectivity, and dependences on temperature and pH as well as operating and storage stabilities. The sensor has a variety of characteristics including good sensitivity, rapid response time, and low detection limit of 5.0 mu M. (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:46:03