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Titolo:
INSULIN ACTIVATION OF PHOSPHATIDYLINOSITOL 3-KINASE IN HUMAN SKELETAL-MUSCLE IN-VIVO
Autore:
HICKEY MS; TANNER CJ; ONEILL DS; MORGAN LJ; DOHM GL; HOUMARD JA;
Indirizzi:
COLORADO STATE UNIV,DEPT EXERCISE & SPORT SCI,MOBY B FT COLLINS CO 80523 E CAROLINA UNIV,SCH MED,HUMAN PERFORMANCE LAB GREENVILLE NC 27858 E CAROLINA UNIV,SCH MED,DEPT BIOCHEM GREENVILLE NC 27858
Titolo Testata:
Journal of applied physiology
fascicolo: 3, volume: 83, anno: 1997,
pagine: 718 - 722
SICI:
8750-7587(1997)83:3<718:IAOP3I>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR SUBSTRATE-1; GLUCOSE-TRANSPORT; INTACT RAT; LIVER; PHOSPHORYLATION; ASSOCIATION; RESISTANCE; WORTMANNIN; ADIPOCYTES; CELLS;
Keywords:
INSULIN SIGNALING; GLUCOSE; MUSCLE BIOPSY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
M.S. Hickey et al., "INSULIN ACTIVATION OF PHOSPHATIDYLINOSITOL 3-KINASE IN HUMAN SKELETAL-MUSCLE IN-VIVO", Journal of applied physiology, 83(3), 1997, pp. 718-722

Abstract

The purpose of this investigation was to determine whether insulin-stimulated phosphatidylinosital 3-kinase (PI3-kinase) activity is detectable in needle biopsies of human skeletal muscle. Sixteen healthy nonobese males matched for age, percent fat, fasting insulin, and fasting glucose participated in one of two experimental protocols. During an intravenous glucose tolerance test (IVGTT) protocol, insulin-stimulatedPI3-kinase activity was determined from percutaneous needle biopsies at 2, 5, and 15 min post-insulin administration (0,025 U/kg). In the second group, a 2-h, 100 mu.m(-2).min(-1) euglycemic hyperinsulinemic clamp was performed, and biopsies were obtained at 15, 60: and 120 min after insulin infusion was begun. Insulin stimulated PIS-kinase activity by 1.6 +/- 0.2-, 2.2 +/- 0.3-, and 2.2 +/- 0.4-fold at 2, 5, and 15min, respectively, during the IVGTT: During the clamp protocol, PI3-kinase Nas elevated by 5.3 +/- 1.3-, 8.0 +/- 2.6-, and 2.1 +/- 1.4-foldabove basal at 15, 60, and 120 min, respectively. Insulin-stimulated PI3-kinase activity at 15 min post-insulin administration was significantly greater during the clamp protocol vs. the IVGTT (P < 0.05). These observations suggest that insulin-stimulated PI3-kinase activity is detectable in needle biopsies of human skeletal muscle, and furthermore, that the euglycemic, hyperinsulinemic clamp protocol may be a useful tool to assess insulin signaling in vivo.

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Documento generato il 03/12/20 alle ore 05:58:37