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Titolo:
COMPARISON OF 5 TESTS FOR THE DETECTION OF ANTIBODIES AGAINST CHLAMYDIAL (ENZOOTIC) ABORTION OF EWES
Autore:
JONES GE; LOW JC; MACHELL J; ARMSTRONG K;
Indirizzi:
MOREDUN RES INST,408 GILMERTON RD EDINBURGH EH17 7JH MIDLOTHIAN SCOTLAND EDINBURGH VET INVEST CTR,SAC VET SERV PENICUIK EH26 0QE MIDLOTHIAN SCOTLAND
Titolo Testata:
Veterinary record
fascicolo: 7, volume: 141, anno: 1997,
pagine: 164 - 168
SICI:
0042-4900(1997)141:7<164:CO5TFT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; SHEEP FLOCK; PSITTACI; ORIGIN; OVINE; DIAGNOSIS; PECORUM; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
G.E. Jones et al., "COMPARISON OF 5 TESTS FOR THE DETECTION OF ANTIBODIES AGAINST CHLAMYDIAL (ENZOOTIC) ABORTION OF EWES", Veterinary record, 141(7), 1997, pp. 164-168

Abstract

Five tests for antibodies against chlamydial (enzootic) abortion of ewes were compared using 255 sera from experimentally (group 1) or naturally (group 2) infected animals, flocks free of the disease (group 3)and individual animals testing positively by the complement fixation test but from flocks with no evidence of chlamydial abortion (group 4)Sera from five specific pathogen-free lambs vaccinated with two different subtypes of Chlamydia pecorum were also included (group 5), AU tests used some form of processed culture of C psittaci as antigen, Specificities, established with groups 3 and 4 sera, ranged between 96 percent (ELISA using lipopolysaccharide antigen) and 59 per cent (Immunocomb). Reactions with group 5 sera suggested that the cause of false positive results in the field might be cross-reactive antibodies against tile arthritogenic subtype of C pecorum. Sensitivities, Established with groups 1 and 2 sera, ranged between 81 per cent (Immunocomb) and 51 per cent (ELISA using solubilised protein antigen), The minimum sample sizes required to be 95 per cent certain of detecting at least five seropositives in two infected flocks (combined data) were 15 to 48, dependent on the test applied, The Western blot test, applied to a proportion of samples, yielded no false positives with group 3 sera bat 31.7 per cent with group 4 sera, Thus, none of the tests in this comparison emerged as sufficiently satisfactory in all respects, suggesting that further improvements in chlamydial serology must come through theuse of non-native antigens or in the form of a competitive ELISA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 04:14:01